Hgc 27

This study was approved by the Ethics Committee of Heilongjiang Provincial Hospital and was conducted in accordance with the ethical principles of the Declaration of Helsinki. Upon receiving signed informed consent, GC tissues and nontumor gastric mucosa were collected from 49 patients at our hospital. No patients had received any type of anticancer therapy. After obtaining the tissues during surgery, they were stored in liquid nitrogen until further use.
The human gastric epithelial cell line GES-1 (Beyotime; Shanghai, China) and GC cell lines SNU-1 and HGC27 (National Collection of Authenticated Cell Cultures; Shanghai, China) were grown in Roswell Park Memorial Institute Medium-1640 (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS). F12K medium (Gibco; Thermo Fisher Scientific) was used for the GC cell line AGS (National Collection of Authenticated Cell Cultures). All basal media were supplemented with 10% FBS and 1% penicillin–streptomycin (Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in a humidified incubator at 37°C under 5% CO₂.

Gastric cancer cells NUGC3 and HGC27 were obtained from the National Collection of Authenticated Cell Cultures and cultured in RPMI-1640 medium (Biological Industries, Israel), which was supplemented with 10% fetal bovine serum (FBS, MEILUNCELL, China), 100 mg/mL penicillin, and 100 mg/mL streptomycin (Biological Industries, Israel). Cells were cultured in an incubator with 5% CO₂ at 37 °C. Depending on the status of the cells' development, the medium was periodically adjusted. Cells in the logarithmic growth phase were used for subsequent experiments.

Normal human GES-1 cell line and five gastric cancer cell lines (MGC-803, AGS, HGC-27, SGC-7901, and BGC-823) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (China). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, USA) with 5% fetal bovine serum (Thermo Scientific HyClone, China), in a humidified incubator (Thermo, USA) with 5% CO², 95% air.

Human gastric cancer cell lines HGC-27 and AGS were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Human normal gastric mucosal epithelium cell lines GES-1 was supplied by The First Hospital of China Medical University (Shenyang, China). All cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C.

Seven gastric cancer cell lines (AGS, MGC80-3, BGC-823, HGC-27, MKN-28, MKN-45, SGC-7901) and a gastric mucosal cell line (GES-1) were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). HGC27, MKN-28, and MKN-45 cells were cultured in Dulbecco’s Minimum Essential Medium and the other cell lines were cultured in RPMI-1640 Medium, supplemented with 10% fetal bovine serum at 37 °C in a humidified atmosphere containing 5% CO₂. All of the culture media were purchased from Sigma (St. Louis, MO, USA), and fetal bovine serum was purchased from Gibco (catalogue no. 16000-044; Grand Island, NY, USA).

GC cell lines MKN-45 and MKN-28, and immortalized human gastric epithelial cell line GES-1, were obtained from 3D Biopharm Biotech Co. Ltd. (Shanghai, China). NCI-N87, HGC-27, AGS, and SGC-7901 cell lines were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). SNU-216 and GTL-16 cell lines were gifts from the Medical College of Xiamen University (Xiamen, China) and AstraZeneca China R&D Center (Shanghai, China). EBC-1 cell line was obtained from COBIOER BIOSCIENCES Co. Ltd (Nanjing, China). Cell lines were tested and authenticated by short tandem repeat DNA profiling analysis before execution of the experiments. Cells were cultured in Minimum Essential Medium (HGC-27), F12K medium (AGS), Dulbecco's Modified Eagle's Medium (GTL-16), Eagle's Minimum Essential Medium (EBC-1) or Roswell Park Memorial Institute 1640 medium containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA) and 1% penicillin-streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C in a humidified atmosphere with 5% carbon dioxide.