Super sensitive polymer hrp ihc detection kit

Manufactured by BioGenex

Sourced in United States

The Super Sensitive Polymer-HRP IHC detection kit is a laboratory equipment product designed for immunohistochemistry (IHC) analysis. It utilizes a polymer-based horseradish peroxidase (HRP) detection system to amplify the signal during the IHC staining process. The kit is intended to provide a sensitive and reliable method for the detection of target antigens in tissue samples.

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Super Sensitive™ Polymer-HRP IHC Super sensitive detection kit Polymer-HRP kit HRP-detection kit IHC kit

2 protocols using super sensitive polymer hrp ihc detection kit

Immunohistochemical Analysis of Fibrinogen

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Paraffin sections were heated at 60 °C for 10 min, deparaffinised in xylene and rehydrated via decreasing ethanol concentrations (100%, 100% 90%, 70% and distilled H₂O). Sections were permeabilised and endogenous peroxidase activity quenched in 1% H2O2 in PBS Triton X-100 (PBS-Tx) 0.3% for 30 min, followed by antigen retrieval with 0.01 M citrate buffer (pH 6) for 20 min in a steam bath. Sections were incubated overnight with primary antibody for anti-rabbit fibrinogen (Dako, Denmark, A0080, 1:2000) diluted in PBS-Tx 0.3% at 4 °C. The Super Sensitive Polymer-HRP IHC detection kit (BioGenex, USA) was used according to manufacturer’s protocol; Sections were counterstained with hematoxylin, then dehydrated with increasing concentrations of ethanol (70%, 90%, 100% and 100%) and xylene, and coverslipped with DPX.

Farajzadeh Khosroshahi S., Yin X., K. Donat C., McGarry A., Yanez Lopez M., Baxan N., J. Sharp D., Sastre M, & Ghajari M. (2021). Multiscale modelling of cerebrovascular injury reveals the role of vascular anatomy and parenchymal shear stresses. Scientific Reports, 11, 12927.

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IHC Analysis of CD37 Expression in Lymphoma Samples

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IHC was used to evaluate CD37 expression in human lymphoma microarray samples (US Biomax) of formalin-fixed and paraffin-embedded (FFPE) tissue sections. Briefly, FFPE sections were deparaffinized, rehydrated, treated for antigen retrieval, and incubated with VCD37–9a73.1 (1 µg/mL), an in-house developed mouse anti-CD37 antibody or control IgG (1 µg/mL). Immunoreactivity was detected using a Super-Sensitive Polymer-HRP IHC Detection Kit (Biogenex Laboratories). Positivity was calculated by summing the percentage of tumor cells stained at a given staining intensity (0, negative; 1, weak; 2, moderate; 3, strong) multiplied by the weighted intensity to yield: high (201–300), moderate (101–200), low (1–100), and negative (0) H-scores.

Pereira D.S., Guevara C.I., Jin L., Mbong N., Verlinsky A., Hsu S.J., Aviña H., Karki S., Abad J.D., Yang P., Moon S.J., Malik F., Choi M.Y., An Z., Morrison K., Challita-Eid P.M., Doñate F., Joseph I.B., Kipps T.J., Dick J.E, & Stover D.R. (2015). AGS67E, an Anti-CD37 Monomethyl Auristatin E Antibody–Drug Conjugate as a Potential Therapeutic for B/T-Cell Malignancies and AML: A New Role for CD37 in AML. Molecular cancer therapeutics, 14(7), 1650-1660.

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