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Atropine sulphate

Manufactured by Mitsubishi
Sourced in Japan

Atropine sulphate is a chemical compound used as a laboratory reagent. It is a salt formed by the combination of the organic compound atropine and sulfuric acid. Atropine sulphate is commonly used in various scientific and research applications.

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2 protocols using atropine sulphate

1

Canine Lung Mass Resection Protocol

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General anaesthesia was pre‐administered by subcutaneous injection of 0.04 mg/kg atropine sulphate (Mitsubishi Tanabe Pharma Co., Osaka, Japan), followed by intravenous injection of 0.1 mg/kg midazolam (Dormicum; Astellas Pharma Inc., Tokyo, Japan) and 0.1 ml/kg fentanyl citrate‒droperidol (Thalamonal; Daiichi‐Sankyo Propharma Co., Ltd., Tokyo, Japan). General anaesthesia was induced using propofol (Mylan; Mylan Seiyaku Ltd., Tokyo, Japan). Endotracheal intubation was subsequently performed. Each dog was mechanically ventilated with 2.0%–2.5% isoflurane (IsoFlo; Zoetis Japan, Tokyo, Japan) and oxygen. For analgesia, intra‐ and post‐operative continuous drip infusions of remifentanil (5–40 μg/kg/h) (Ultiva; Janssen Pharmaceutical K.K., Tokyo, Japan) and pre‐ and post‐operative intramuscular injections of morphine hydrochloride (0.3 mg/kg each dose) (Takeda Pharmaceutical Co. Ltd. Osaka, Japan) were used.
Prior to surgery, CT was performed, and the volume (cm3) of the lung mass was measured in terms of length, width, and height. All patients were approached through an intercostal or median sternotomy depending on the region and size of the individual tumours, and the tumours were resected by lung lobectomy. All resected masses were weighed.
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2

Healthy Dog Lung Tissue Procurement

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Normal lung tissue for real‐time PCR was collected from five dogs (Beagle, individual numbers: 5FW455, 5FW471, 5FW494, 5FW500, and 5FW504) approved by the ethics committee at our institution. These dogs underwent blood tests, and radiography revealed that they were healthy. General anaesthesia was pre‐administered by subcutaneous injection of 0.04 mg/kg atropine sulphate (Mitsubishi Tanabe Pharma Co.), followed by intravenous injection of 0.1 mg/kg midazolam (Dormicum; Astellas Pharma Inc.) and 0.2 mg/kg butorphanol (Butorphanol; Meiji Seika Pharma Inc., Tokyo, Japan). General anaesthesia was induced using propofol (Mylan; Mylan Seiyaku Ltd.). After induction of anaesthesia, a right fourth intercostal incision was made, and the middle lobe of the right lung was resected. A portion of the obtained lung tissue was frozen with liquid nitrogen and stored at −80°C. The animals were then maintained under deep anaesthesia with isoflurane (5%) and euthanized with intravenous potassium chloride (40 mEq/head).
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