RKO-AS45-1 and HCT-116 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA). RKO-AS45-1 cells were grown in Dulbecco’s modified Eagle’s minimal essential medium (DMEM; Gibco BRL, Gaithersburg, MD, USA). HCT-116 cells were grown in RPMI 1640 (GIbco BRL) with L-Glutamine and 25 mM HEPES. Both cells were supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco BRL), 100 U/ml penicillin and 100 mg/ml streptomycin (Gibco BRL) and kept at 37°C in a 5% CO2/95% air atmosphere. 5-FU and Camptothecin were purchased from Sigma and Cisplatin, Doxorubicin and R428 from Selleckchem. All drugs were solubilized in DMSO. Cells were treated with the drugs for 24 hr and cell viability was determined as described below.
Rko as45 1
Manufactured by American Type Culture Collection
The RKO-AS45-1 is a compact and versatile laboratory centrifuge designed for a variety of applications. It features a fixed-angle rotor with a maximum speed of 4,500 RPM and a maximum relative centrifugal force (RCF) of 2,880 × g. The centrifuge can accommodate up to 45 mL sample containers, making it suitable for small-scale sample preparation and separation tasks. The unit is equipped with a digital display, programmable settings, and safety features to ensure reliable and consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Cisplatin, by Selleck Chemicals (1 mentions)
Camptothecin, by Merck Group (1 mentions)
Doxorubicin, by Selleck Chemicals (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Bt 549, by Merck Group (1 mentions)
Tov 21g, by Merck Group (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Tov 21g, by American Type Culture Collection (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Htb 122, by American Type Culture Collection (1 mentions)
Wi 26 va4, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Ccl 95.1, by American Type Culture Collection (1 mentions)
2 protocols using rko as45 1
1
Cell Lines and Cytotoxicity Assay
2
Cell Culture Conditions for Cancer Lines
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Cell lines BT-549 (breast ductal carcinoma; HTB-122™), TOV-21G (ovarian adenocarcinoma; CRL-11730™), RKO-AS45-1 (colorectal carcinoma; CRL-2579™) and WI-26 VA4 (lung fibroblast used as a control; CCL-95.1™) were purchased from the American Type Culture Collection. The BT-549 cell line was cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) inactivated at 56°C for 30 min, 1% 0.2 M L-glutamine and 10 mg/ml bovine insulin (Sigma-Aldrich; Merck KGaA). The TOV-21G cell line was grown in high-glucose Dulbecco's modified Eagle's medium (Sigma-Aldrich; Merck KGaA) supplemented with 15% FBS and 1% 0.2 M L-glutamine, and the RKO-AS45-1 and WI-26 VA4 cell lines were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% 0.2 M L-glutamine. Cells were incubated at 37°C in a humidified atmosphere enriched with 5% CO2. The experiments were carried out obeying a certain passage number (between passages 4 and 6).
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