Plasma antibody responses were measured in two samples, the 2017 one and another 14 to 19 years before for comparison with two long term viremic controllers as controls (#12 and #60) with a follow-up of >10 years with > 500 cells/µl and VL >2,000 cp/µl. End-point plasma titers of HIV-1 antibodies were measured in an enzyme immunoassay (Genscreen™ HIV-1/2 Version 2, Bio-Rad). Antibodies against the HIV-1 proteins were determined by Western Blot (Biokit, Werfen, Spain) and Line Immuno Assay (InnoLia, Fujirebio Europe N.V, Belgium) and quantified with the LiRAS software. Neutralizing HIV-1 antibody titers in purified IgGs were tested against a mini panel of isolates23 (link).
Line immuno assay
Manufactured by Fujirebio
Sourced in Sweden
The Line Immuno Assay is a laboratory equipment used for the qualitative and quantitative determination of analytes in biological samples. It employs immunological principles to detect and measure the presence of specific substances in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Genscreen hiv 1 2 version 2, by Bio-Rad (1 mentions)
Western blot, by Fujirebio (1 mentions)
Sd biolane duo, by Abbott (1 mentions)
Bdsurepath liquid based papanicolaou tests, by BD (1 mentions)
Microlab nimbus automat, by Seegene (1 mentions)
Allplex sti essential assay, by Seegene (1 mentions)
Inno lia hiv 1 2 score 20t, by Fujirebio (1 mentions)
Starmag 96 4 universal cartridge kit, by Seegene (1 mentions)
Elisa, by Inova Diagnostics (1 mentions)
3 protocols using line immuno assay
1
Longitudinal Plasma Antibody Responses
2
STI Screening among Key Populations
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A standardized questionnaire adapted from a Family Health International (FHI) 360 validated guide for bio-behavioral surveys was administered during a face-to face interview to collect information regarding socio-demographic characteristics, risky sexual behaviors.
Cervical and anal swabs were collected respectively from FSW and MSM with Cervex-Brush® Combi and Anex-Brush®, respectively (Rovers Medical Devices, Oss, the Netherlands) and conserved in preservative solution (BDSurePath™ liquid-based Papanicolaou tests, Becton-Dickinson, Franklin Lakes, NJ, USA). These samples were stored at 4 °C and sent at room temperature to France for analyses. Nucleic acids were extracted using a STARMag 96 × 4 Universal Cartridge kit (Seegene, Seoul, Korea) on the MICROLAB NIMBUS automat (Seegene). T. vaginalis and six other STI (N. gonorrhoeae, C. trachomatis, M. genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum) were identi ed using the Allplex STI Essential Assay (Seegene).
Venous blood samples were collected to test for HIV and Syphilis using SD Biolane Duo (Abbott). Each HIV positive case was con rmed with a second rapid diagnosis test, First Response HIV 1-2-O Card Test (Premier Medical Corporation Pvt. Ltd). In case of discordant results, samples were performed with the INNO-LIA HIV I/II Score (20T) (Fujirebio) line immunoassay (Fujirebio, Göteborg, Sweden).
Cervical and anal swabs were collected respectively from FSW and MSM with Cervex-Brush® Combi and Anex-Brush®, respectively (Rovers Medical Devices, Oss, the Netherlands) and conserved in preservative solution (BDSurePath™ liquid-based Papanicolaou tests, Becton-Dickinson, Franklin Lakes, NJ, USA). These samples were stored at 4 °C and sent at room temperature to France for analyses. Nucleic acids were extracted using a STARMag 96 × 4 Universal Cartridge kit (Seegene, Seoul, Korea) on the MICROLAB NIMBUS automat (Seegene). T. vaginalis and six other STI (N. gonorrhoeae, C. trachomatis, M. genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum) were identi ed using the Allplex STI Essential Assay (Seegene).
Venous blood samples were collected to test for HIV and Syphilis using SD Biolane Duo (Abbott). Each HIV positive case was con rmed with a second rapid diagnosis test, First Response HIV 1-2-O Card Test (Premier Medical Corporation Pvt. Ltd). In case of discordant results, samples were performed with the INNO-LIA HIV I/II Score (20T) (Fujirebio) line immunoassay (Fujirebio, Göteborg, Sweden).
3
Autoantibody and Biomarker Profiling
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Antihistone and antinucleosome IgG antibodies were measured at week 5 or 21 by ELISA (Inova Diagnostics, San Diego, CA, USA), using horseradish peroxidase‐conjugated anti‐mouse antibodies (Southern Biotech, Birmingham, AL, USA 1:2000) as secondary antibodies 14 . Additionally, sera were analysed by line immunoassay (Fujirebio Diagnostics, Göteborg, Sweden) for the presence of antibodies against the antigens SmB, SmD, U1‐70K, U1‐A, U1‐C, Ro60. Ro52 (TRIM21), La, topoisomerase I, Jo1, Centromere Protein B, and Ribosomal P protein as previously described 10 . Serum levels of sIL‐6R were determined by ELISA (DY1830, R&D Systems, Minneapolis, MN, USA). Urine albumin levels were investigated using a murine α‐Albumin ELISA quantitation Kit (Cat No.: E90‐134, Bethyl Lab.Inc., Montgomery, TX, USA) at beginning and end of treatment.
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