Log In Sign up
The largest database of trusted experimental protocols

1433 op 050 cf

Manufactured by R&D Systems
Visit Supplier

The 1433-OP-050/CF is a laboratory equipment product. It is a core component used for specific research and development functions. A detailed and unbiased description of the product's core function cannot be provided while maintaining the requested objectivity.

Automatically generated - may contain errors

Lab products found in correlation

Human osteopontin quantikine elisa kit, by R&D Systems (1 mentions) U251 cells, by Merck Group (1 mentions) Human csf 1, by BioLegend (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) U937 cells, by American Type Culture Collection (1 mentions) Vybrant cell adhesion assay kit, by Thermo Fisher Scientific (1 mentions) Thrombin, by Merck Group (1 mentions) Fbs rpmi medium, by Thermo Fisher Scientific (1 mentions) Luna cell counter, by Logos Biosystems (1 mentions)

5 protocols using 1433 op 050 cf

1

Measuring Secreted Osteopontin by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Secreted OPN in the supernatant of cultured cells was measured using the Human Osteopontin Quantikine ELISA kit (DOST00; R and D systems) according to manufacturers protocol. For experiments where exogenous ligand was added, recombinant human OPN (1433-OP-050/CF; R and D Systems) was added at 1 μg/mL and cells assayed 2–3 days later.
Adams C.R., Htwe H.H., Marsh T., Wang A.L., Montoya M.L., Subbaraj L., Tward A.D., Bardeesy N, & Perera R.M. (2019). Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer. eLife, 8, e45313.
+ Open protocol
+ Expand
2

Monocyte-Macrophage Differentiation and Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cell–derived monocytes were obtained from healthy human volunteer donors ages 16 to 64 of all genders, races, and ethnicities at Human Immunology Core at University of Pennsylvania. Informed consent was obtained from all donors under an Institutional Review Board–approved protocol at University of Pennsylvania. Our work with human participants complies with all relevant ethical regulations. Human primary monocytes were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and treated with human CSF-1 (10 ng/ml; BioLegend, 574806) for 5 days to differentiate into macrophages. Cells were treated with recombinant human OPN (R&D Systems, 1433-OP-050/CF), or cocultured (20:1) with ECs isolated from human GBM tumors, human brain microvascular ECs (ScienCell, 1000), or human glioma cells (U251 cells, Sigma-Aldrich, 09063001; U87 cells, Sigma-Aldrich, 89081402) for 2 days in the presence or absence of or OPN-neutralizing antibody (1 μg/ml; R&D Systems, AF1433-SP).
Yang F., Akhtar M.N., Zhang D., El-Mayta R., Shin J., Dorsey J.F., Zhang L., Xu X., Guo W., Bagley S.J., Fuchs S.Y., Koumenis C., Lathia J.D., Mitchell M.J., Gong Y, & Fan Y. (2024). An immunosuppressive vascular niche drives macrophage polarization and immunotherapy resistance in glioblastoma. Science Advances, 10(9), eadj4678.
+ Open protocol
+ Expand
3

Macrophage Polarization and Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
U937 cells (ATCC) were cultured in the RPMI 1640 media 24 h before priming. U937 monocytes were primed with 100 nM Phorbol 12-myristate 13-acetate (PMA, Sigma) for 48 h to become monocyte-derived macrophages. Transwell assays assessing cell migration potential were performed on 24-well plates with inserts (BD Biosciences) according to the manufacturer's instruction. Briefly, 5×10 5 primed U937 cells were cultured in the upper chamber and allowed to migrate for 24-48 h before fixation for crystal purple staining. Recombinant human SPP1 protein was purchased from R&DSystems (1433-OP-050/CF). Conditional media were obtained by culturing U251 and A1207 cells in DMEM media for 48 h and then used for the cell migration Transwell and M2 polarization assay. For the M2 polarization experiment with U937 cells, we cultured the U937 cells in conditioned medium for 48 h. Then, the cells were collected for the detection of M2 markers in subsequent experiments.
Yang Y., Jin X., Xie Y., Ning C., Ai Y., Wei H., Xu X., Ge X., Yi T., Huang Q., Yang X., Jiang T., Wang X., Piao Y, & Jin X. (2024). The CEBPB+ glioblastoma subcluster specifically drives the formation of M2 tumor-associated macrophages to promote malignancy growth. Theranostics, 14(10).
+ Open protocol
+ Expand
4

NALM-6 Cell Adhesion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
NALM-6 cells were pre-treated with propofol and sevoflurane with respective concentrations. Recombinant human OPN (R&D systems, 1433-OP-050/CF) was thrombin-cleaved by incubating 24 μg recombinant full length human OPN in 20 mM Tris-HCL (pH7.6), 80 mM NaCl, 2 mM CaCl
2, and 0.1 unit thrombin (Merck, 10602400001) for ten minutes at 37 degrees. Then, full length OPN and thrombin-cleaved OPN were coated at 20 μg/ml the night before experiment. Then, 5 × 10
5 cells of NALM-6 cells were labelled with calcein-AM stock solution from the Vybrant cell adhesion assay kit (ThermoFisher, UK). Pre-labelled NALM-6 cells were allowed to adhere for two hours under 37°C. Finally, fluorescence readings were read at 494 nm. The percentage of adhesion was determined by dividing the corrected (background subtracted) fluorescence of adherent cells by the total corrected fluorescence of cells added to each microplate well and multiplying by 100%.
Jiang C., Gonzalez-Anton S., Li X., Mi E., Wu L., Zhao H., Zhang G., Lu A., Lo Celso C, & Ma D. (2024). General anaesthetics reduce acute lymphoblastic leukaemia malignancies in vitro and in vivo via CXCR4 and osteopontin mediated mechanisms. F1000Research, 11, 1491.
+ Open protocol
+ Expand
5

Prostate Cell Line Response to Osteopontin

Check if the same lab product or an alternative is used in the 5 most similar protocols
The P69 human prostate cell line was obtained through Dr. Frédéric Bost’s lab (C3M, Inserm U1065, France). P69 (passage 16 to 18) cells were cultured in 10% FBS RPMI medium (Invitrogen). Cells were treated with recombinant human OPN protein (R&D systems, 1433-OP-050/CF, Minneapolis, Minnesota) at concentrations of 0.1, 1, or 10 mg/mL or PBS as negative control for 24 hours. After trypsinization, cell suspension count was performed on an automated LUNA cell counter (Logos Biosystems, Villeneuve d’Ascq, France).
Bousset L., Septier A., Bunay J., Voisin A., Guiton R., Damon-Soubeyrant C., Renaud Y., De Haze A., Sapin V., Fogli A., Rambur A., De Joussineau C., Kocer A., Trousson A., Henry-Berger J., Höring M., Liebisch G., Matysik S., Lobaccaro J.M., Morel L, & Baron S. (2020). Absence of nuclear receptors LXRs impairs immune response to androgen deprivation and leads to prostate neoplasia. PLoS Biology, 18(12), e3000948.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!