Pe conjugated rat anti mouse gr 1

Manufactured by BD

Sourced in Australia

PE-conjugated rat anti-mouse Gr-1 is a laboratory reagent used for the detection and analysis of myeloid-derived suppressor cells (MDSCs) in mouse samples. It is a monoclonal antibody that specifically binds to the Gr-1 (Ly-6G/Ly-6C) antigen expressed on the surface of mouse granulocytes and monocytes. The antibody is conjugated with the fluorescent dye phycoerythrin (PE), allowing for the visualization and quantification of Gr-1-positive cells through flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Fluorescence microscopy, by Nikon (1 mentions) Fitc conjugated rat anti mouse cd11b, by BD (1 mentions) Fitc conjugated rat anti mouse cd4, by BD (1 mentions) Pe conjugated rat anti mouse foxp3, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)

Spelling variants of this product

Anti-Gr-1 (49 citations) Anti-Gr-1-PE (13 citations) Rat anti-mouse Gr-1 (4 citations) Anti-mouse Gr-1 (4 citations) PE-conjugated anti-mouse Gr-1 (3 citations) Mouse anti (2 citations)

2 protocols using pe conjugated rat anti mouse gr 1

Flow Cytometry Analysis of Immune Cells

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Single-cell suspensions from spleens or tumours were stained with PE-conjugated rat anti-mouse Gr-1, fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse CD11b, PE-conjugated rat anti-mouse F4/80, or FITC-conjugated rat anti-mouse CD11c antibodies (all purchased from BD Biosciences). Isotype-matched antibodies were used as controls. Samples were analysed using the Gallios and the Kaluza software (Beckman Coulter).

Nishio H., Yaguchi T., Sugiyama J., Sumimoto H., Umezawa K., Iwata T., Susumu N., Fujii T., Kawamura N., Kobayashi A., Park J., Aoki D, & Kawakami Y. (2014). Immunosuppression through constitutively activated NF-κB signalling in human ovarian cancer and its reversal by an NF-κB inhibitor. British Journal of Cancer, 110(12), 2965-2974.

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Multicolor Immunofluorescence Staining of Tumor Microenvironment

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Tissue section slides were processed via deparaffinization, antigen retrieval, permeabilization, and blocking with 1% bovine serum albumin (BSA) at room temperature for 1 h. MDSCs were defined using FITC-conjugated rat-anti-mouse CD11b and PE-conjugated rat-anti-mouse Gr1 (BD, New South Wales, Australia). Tregs were defined using FITC-conjugated rat-anti-mouse CD4 and PE-conjugated rat-anti-mouse Foxp3 (BD, New South Wales, Australia). T cells were defined using FITC-conjugated rat-anti-mouse CD8a (BD, New South Wales, Australia). Vessels and tumor-associated fibroblasts (TAF) were characterized by rabbit-anti-mouse CD31 and rabbit-anti-mouse α-SMA, and then treated with Alexa Fluor647-conjugated goat-anti-rabbit antibody. Nuclei were counterstained with DAPI (Vector Laboratories Inc, Burlingame, CA). All commercial binding reagents were diluted according to the manufacturer’s recommendation. Images were taken using fluorescence microscopy (Nikon, Tokyo, Japan). Three randomly selected microscopic fields were quantitatively analyzed on ImageJ software.

Huo M., Zhao Y., Satterlee A.B., Wang Y., Xu Y, & Huang L. (2016). Tumor-targeted delivery of sunitinib base enhances vaccine therapy for advanced melanoma by remodeling the tumor microenvironment. Journal of controlled release : official journal of the Controlled Release Society, 245, 81-94.

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