Mgcl2 and dntps

Manufactured by Takara Bio

Sourced in Japan

MgCl2 and dNTPs are fundamental reagents used in various molecular biology applications. MgCl2 is an essential cofactor for numerous enzymatic reactions, including DNA replication and amplification. dNTPs, or deoxynucleotide triphosphates, are the building blocks for DNA synthesis. These products provide the necessary components for essential molecular biology procedures.

Automatically generated - may contain errors

Lab products found in correlation

Bst dna polymerase, by New England Biolabs (3 mentions) Hydroxynaphthol blue hnb, by Merck Group (2 mentions) Betaine, by Merck Group (1 mentions) Escherichia coli er2738, by New England Biolabs (1 mentions) Isopropyl β d thiogalactoside, by Merck Group (1 mentions) Mouse anti m13 monoclonal antibody horse radish peroxidase conjugate, by GE Healthcare (1 mentions)

3 protocols using mgcl2 and dntps

Fungicide Resistance Profiling in Fusarium

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Fusarium graminearum isolate R9 (Table S2), which is resistant to MBC because of a point mutation at codon 167 (Phe to Tyr, F167Y) in the β₂-tubulin gene (Genbank accession no. FJ214663), and F. graminearum isolate 2021 (Table S2), which is sensitive to MBC, were used in this study. Other F. graminearum isolates were collected from diseased wheat spikelets from different geographical regions in China (Table 2, 3).
Carbendazim was provided by the Shenyang Academy of Chemistry and Industry, China. The fungicide was dissolved in 0.1 M hydrochloric acid (HCl) and adjusted to 1 mg mL⁻¹. Bst DNA polymerase was purchased from NEB. Betaine and hydroxynaphthol blue (HNB) were purchased from Sigma, and MgCl₂ and dNTPs were purchased from Takara. Double-distilled water (ddH₂O) was used in all experiments. All other reagents were analytical grade.

Duan Y., Zhang X., Ge C., Wang Y., Cao J., Jia X., Wang J, & Zhou M. (2014). Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum. Scientific Reports, 4, 7094.

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Phage Display for Anti-OP Pesticide mAb

Cited 2 times

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All reagents were of analytical grade unless
specified otherwise. Parathion-methyl, chlorpyrifos-methyl, azinphos-methyl,
dimethoate, fenitrooxon, EPN, paraoxon-ethyl, paraoxon-methyl, dicapthon,
cyanophos, and famphur were all purchased from Dr. Ehrenstorfer (Germany).
Other pesticide standards were provided by the Jiangsu Pesticide Research
Institute (China). Anti-OP pesticide mAb C8/D3 was produced in our
laboratory.^{35 (link)} Mouse anti-M13 monoclonal
antibody–horse radish peroxidase (HRP) conjugate was purchased
from GE Health Care (Piscataway, NJ, U.S.A.). Bst DNA polymerase and Escherichia coli ER2738 were
purchased from New England Biolabs (Ipswich, MA, U.S.A.). The cyclic
8-amino-acid random peptide library was developed in the laboratory
(UC Davis, CA, U.S.A.) previously.^{34 (link)} Isopropyl-β-d-thiogalactoside (IPTG), 5-bromo-4-chloro-3-indolyl-β-d-galactoside (Xgal), betaine, and hydroxynaphthol blue (HNB)
were purchased from Sigma (U.S.A.). MgCl₂ and dNTPs were
purchased from Takara (Japan). Double-distilled water was used in
all experiments.

Hua X., Yin W., Shi H., Li M., Wang Y., Wang H., Ye Y., Kim H.J., Gee S.J., Wang M., Liu F, & Hammock B.D. (2014). Development of Phage Immuno-Loop-Mediated Isothermal Amplification Assays for Organophosphorus Pesticides in Agro-products. Analytical Chemistry, 86(16), 8441-8447.

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Isolation and Maintenance of Botrytis cinerea

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B. cinerea isolates used in this work were collected from infected host leaves and fruits listed in Table 1. All tested B. cinerea isolates were obtained by the single-spore method. Other plant pathogens used in this study were maintained in the Laboratory of Plant Disease Control and Phytopharmacy, Nanjing Agricultural University, China, and are listed in Table 2.
Bst DNA polymerase was purchased from NEB. Betaine and hydroxynaphthol blue (HNB) were purchased from Sigma, and MgCl₂ and dNTPs were purchased from Takara. Double-distilled water (ddH₂O) was used in all experiments. All other reagents were analytical grade.

Duan Y.B., Ge C.Y., Zhang X.K., Wang J.X, & Zhou M.G. (2014). Development and Evaluation of a Novel and Rapid Detection Assay for Botrytis cinerea Based on Loop-Mediated Isothermal Amplification. PLoS ONE, 9(10), e111094.

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