HPLC analyses were conducted using ChemStation on an Agilent 1260 quaternary pump and Agilent 1260 Autosampler with DAD UV-vis detector, with a path length of 1.0 cm. Samples were separated using a Phenomenex Kinetex 2.6 mm xB-C18 100 Å, LC column 150 × 2.1 mm. Column temp: 25 °C. 10 µL Injection. Solvents: Solvent gradient was: (A) 0.1% formic acid in LC-MS grade water, (B) LC-MS grade acetonitrile. Flow Rate: 0.3 mL/min. Gradient: 5 min 100% A, 0% B; 20 min ramp to 45% A, 55% B; 10 min 0% A, 100% B; 1 min ramp 100% A, 0% B; 14 min 100% A, 0% B. Wavelengths monitored: 210 and 220 nm, with entire spectrum 180–400 nm collected in 2 nm steps. Processing of HPLC data were conducted using either Excel or a suite of macros within Igor Pro 8.0 (Wavemetrics).
1260 autosampler
Manufactured by Agilent Technologies
Sourced in United States
The 1260 autosampler is a lab equipment product designed for automated sample handling. It is capable of reproducibly injecting liquid samples into an analytical instrument for analysis. The core function of the 1260 autosampler is to provide consistent and reliable sample introduction to support analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
Micromist nebulizer, by Agilent Technologies (2 mentions)
1260 infinity series stack, by Agilent Technologies (2 mentions)
Igor pro 8, by Wavemetrics (1 mentions)
Kinetex 2.6 mm xb c18100, by Phenomenex (1 mentions)
1200 infinity lc system, by Agilent Technologies (1 mentions)
1260 binary pump and degasser, by Agilent Technologies (1 mentions)
1260 variable wavelength detector, by Agilent Technologies (1 mentions)
Lunar c18 column, by Phenomenex (1 mentions)
Hplc system, by Agilent Technologies (1 mentions)
Krudkatcher ultra hplc in line filter, by Phenomenex (1 mentions)
Kinetex c18 reverse phase column, by Phenomenex (1 mentions)
Agilent 1200 hplc system, by Agilent Technologies (1 mentions)
Trace 1300 isq, by Thermo Fisher Scientific (1 mentions)
Laccase from trametes versicolor, by Merck Group (1 mentions)
Tuning solution, by Agilent Technologies (1 mentions)
Model 1260, by Agilent Technologies (1 mentions)
Agilent 7700x icp ms, by Agilent Technologies (1 mentions)
6 protocols using 1260 autosampler
1
HPLC Analysis of Chemical Compounds
2
Arsenic Speciation Analysis in Rice
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The chromatographic separation of arsenite (As(III)), arsenate (As(V)), MMA, DMA and arsenobetaine (AsB) was performed using a 10 mM ammonium phosphate dibasic buffer with pH adjusted to 8.25 on an Agilent 1200 Infinity LC system consisting of a 1260 Isocratic pump and 1260 Auto sampler. The LC system was linked to the Agilent 7700x ICP-MS via Peek tubing and equipped with a low flow Micro Mist Nebulizer and quartz, low-volume Scott-type double-pass spray chamber. The mobile phase was pumped at 1 ml/min and injection volume was set at 100 μL.
Instrument run conditions are listed inTable 5 .
Statistical analyses of all data were done using Excel and VassarStats.39 The measured As species in the CRM for ICP-MS analysis were always within the standard deviation of the CRM (Table 6 ). The coefficient of variation of replicate As species analysis for individual rice samples was usually less than 10% (Table 7 ). The coefficient of variation was highest for As (III).
Instrument run conditions are listed in
Statistical analyses of all data were done using Excel and VassarStats.39 The measured As species in the CRM for ICP-MS analysis were always within the standard deviation of the CRM (
3
HPLC Analysis of Organic Compounds
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HPLC was performed using an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump and degasser. The flow rate was set to 1.0 mL min−1 and samples were injected using Agilent 1260 autosampler with a 20 μL injection volume. The temperature of the column was set at 37 °C. The HPLC was fitted with a phenomenex Lunar C18 column (150 × 4.6 mm) with 5 m packing (100Ǻ). Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was ‘far UV’) and consisted of mobile phase A: 100% ACN, 0.04% TFA; mobile phase B: 100% water, 0.04% TFA with a gradient of 5 to 95% ACN over 30 min. All data was elaborated using Prism9.
4
HPLC-MS/MS Metabolite Analysis
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The HPLC system (Agilent, Santa Clara, CA, USA) consisted of an Agilent 1200 binary pump, DAD, and Agilent 1260 autosampler. A Kinetex C18 reverse-phase column (5 μm, 4.6 × 150 mm) (Phenomenex, Torrance, CA, USA) protected by a KrudKatcher Ultra HPLC in-line filter (Phenomenex) was used for separation. HPLC conditions to determine the isozyme-specific metabolites and major constituents in OFIE are described in the Supplementary Materials . LC-electrospray (ESI)-tandem mass spectrometry (MS2) analysis was employed to detect glucuronide products. An Linear Trap Quadropole (LTQ) linear ion trap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with an ESI source was coupled with an Agilent 1200 HPLC system. MS conditions used to detect glucuronides are summarized in Table S2 .
5
Laccase-Catalyzed Polymer Synthesis and Characterization
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Laccase from Trametes versicolor (≥0.5 U/mg) was supplied by Sigma-Aldrich and purified by dialysis against water for one day following lyophilization. NIPAM (97%, Aladdin) was recrystallized from hexane before use to remove the inhibitor. Glycidyl acrylate (GA), tris(2-
propanamide (DOPA-Br) were synthesized according to literature procedure and stored in the freezer under a nitrogen atmosphere. 44, [60] [61] [62] The functional dye, rhodamine B 4-(3-(Nhydroxysuccinimidyloxocarbonyl)-propyl)piperazine amide (Rhod B) was synthesized according to previous procedure for the labelling of laccase directly. 63 Copper(I) bromide (CuBr, 98%, Aladdin) was washed sequentially with acetic acid and ethanol and dried under vacuum. Titanium dioxide (anatase 99.8% metals basis, average particle size: 25 nm, Aladdin) was dried in vacuum at 120 °C for 12 hours before use. was acquired from FL3-TCSPC fluorescence spectrometer (Horiba Jobin Yvon). Gas chromatography -mass spectrometry (GC-MS) was performed using a Thermo fisher spectrometer (Trace 1300-ISQ) with heating rate of GC columns increased from 50 ℃ to 200 ℃ in eight minutes.
The HPLC system was an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump, mixer and degasser. Samples were injected using an Agilent 1260 autosampler and detection was achieved using UV and fluorescence detector.
propanamide (DOPA-Br) were synthesized according to literature procedure and stored in the freezer under a nitrogen atmosphere. 44, [60] [61] [62] The functional dye, rhodamine B 4-(3-(Nhydroxysuccinimidyloxocarbonyl)-propyl)piperazine amide (Rhod B) was synthesized according to previous procedure for the labelling of laccase directly. 63 Copper(I) bromide (CuBr, 98%, Aladdin) was washed sequentially with acetic acid and ethanol and dried under vacuum. Titanium dioxide (anatase 99.8% metals basis, average particle size: 25 nm, Aladdin) was dried in vacuum at 120 °C for 12 hours before use. was acquired from FL3-TCSPC fluorescence spectrometer (Horiba Jobin Yvon). Gas chromatography -mass spectrometry (GC-MS) was performed using a Thermo fisher spectrometer (Trace 1300-ISQ) with heating rate of GC columns increased from 50 ℃ to 200 ℃ in eight minutes.
The HPLC system was an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump, mixer and degasser. Samples were injected using an Agilent 1260 autosampler and detection was achieved using UV and fluorescence detector.
6
Ultrasensitive Thallium Speciation by IC-ICP-MS
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An Agilent 7700x ICP-MS (Agilent Technologies, USA) with a MicroMist nebulizer and a Peltier cooled (2°C) quartz Scott-type double pass spray chamber was used for the detection of 203 Tl and 205 Tl. The operating parameters for ICP-MS were optimized prior to the determinations with a tuning solution (Agilent Technologies, Tokyo, Japan) containing 1.0 ng/mL of Ce, Co, Li, Mg, Tl and Y in 2% HNO3. A solution of 10 µg/L of Ir in 2% HNO3 was used as internal standard.
Redox Tl speciation was carried out by IC-ICP-MS using a cation exchange guard-column Dionex CG-2 (4.0 mm i.d. 50 mm length). A high-pressure LC pump (1260 model, Agilent, USA) equipped with a 1260 autosampler was used. The mobile phase was 5 mmol/L HNO3 with 3 mmol/L NH4NO3 and 0.75 mmol/L DTPA. The injection volume was 20 µL and the pump flow rate was set at 1.5 mL/min. Limit of detection (LOD), defined as the minimum measured concentration distinguishable from blank, as low as 0.05 µg•L -1 was achieved for Tl(I) and Tl(III)-DTPA.
Redox Tl speciation was carried out by IC-ICP-MS using a cation exchange guard-column Dionex CG-2 (4.0 mm i.d. 50 mm length). A high-pressure LC pump (1260 model, Agilent, USA) equipped with a 1260 autosampler was used. The mobile phase was 5 mmol/L HNO3 with 3 mmol/L NH4NO3 and 0.75 mmol/L DTPA. The injection volume was 20 µL and the pump flow rate was set at 1.5 mL/min. Limit of detection (LOD), defined as the minimum measured concentration distinguishable from blank, as low as 0.05 µg•L -1 was achieved for Tl(I) and Tl(III)-DTPA.
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