Colistin

Manufactured by Solarbio

Sourced in China

Colistin is a bactericidal antibiotic used in laboratory settings to inhibit the growth of certain Gram-negative bacteria. It acts by disrupting the bacterial cell membrane, leading to cell death. Colistin is commonly used in microbiological research and antimicrobial susceptibility testing.

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Lab products found in correlation

Meropenem mem, by Solarbio (2 mentions) Meropenem, by Solarbio (1 mentions) Tigecycline, by Maclin Biochemical Technology (1 mentions) Imipenem, by Solarbio (1 mentions) Gentamicin, by Solarbio (1 mentions) Florfenicol, by Solarbio (1 mentions) Polymyxin b, by Solarbio (1 mentions)

4 protocols using colistin

CRKP Antimicrobial Susceptibility Testing

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The antimicrobial susceptibility testing of the CRKP from HWW, recipient and transconjugants was performed by Vitek-2 (Vitek-AST-GN16) (BioMerieux, France) according to the Clinical and Laboratory Standards Institute guidelines (CLSI, 2022). The antibiotics included ampicillin, amoxicillin/clavulanic acid, piperacillin/tazobactam, cefazolin, cefoxitin, ceftriaxone, cefepime, aztreonam, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, nitrofurantoin, and trimethoprim/sulfamethoxazole. Among them, MICs of ertapenem (Macklin, China), imipenem (Solarbio, China), meropenem (Solarbio, China), tigecycline (Macklin, China) and colistin (Solarbio, China) were determined using broth dilution method. E. coli ATCC 25,922 was used as quality control.

Xie Z., Huang J., zhang S., Xu B., Zhang Q, & Li B. (2023). Genomic and functional characterization of carbapenem-resistant Klebsiella pneumoniae from hospital wastewater. BMC Microbiology, 23, 115.

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Antimicrobial Susceptibility Testing of MNPECs

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Antimicrobial susceptibility testing (AST) was performed by broth microdilution method as our previously described (Liu et al., 2021b (link)). Briefly, 0.5 McFarland inoculum suspensions were diluted 1:100 to a final inoculum density of 10⁶ CFU/ml. Ampicillin (AMP), ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZM), tetracycline (TET), doxycycline (DOX), ciprofloxacin (CIP), enrofloxacin (ENR), gentamicin (GEN), sulfamethoxazole (SMZ), fosfomycin (FOS), florfenicol (FFC), colistin (COL), and meropenem (MEM) (Solarbio, Beijing, China) were selected to determine the minimum inhibitory concentrations (MICs) of MNPECs. The MICs were recorded as the lowest concentrations of antimicrobials in the wells, where no visible bacteria growth was observed. Escherichia coli ATCC 25922 was used as standard reference strains.

Xiang Y., Liu Z., Yu G., Song Y., Li Y., Geng X., Ma L., Guo J., Tan L, & Chen P. (2023). Genetic characteristic of coexisting of mcr-1 and blaNDM-5 in Escherichia coli isolates from lesion-bearing animal organs. Frontiers in Microbiology, 14, 1116413.

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Antimicrobial Peptides Evaluation

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Synthesized AMPs such as Iseganan (IB), Melittin (GQ) and Cathelicidin-DM (SA) were procured from Dangang Biotechnology Company. Polymyxin B (PB) and colistin (CST) were acquired from Solarbio. Specific information about these five AMPs are shown in Table 1.

Zhao C., Yan S., Luo Y., Song Y, & Xia X. (2024). Analyzing resistome in soil and Human gut: a study on the characterization and risk evaluation of antimicrobial peptide resistance. Frontiers in Microbiology, 15, 1352531.

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Antimicrobial Susceptibility Testing Protocol

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Antimicrobial susceptibility testing was performed by broth microdilution method, as internationally recognized clinical guidelines those provided by the Clinical and Laboratory Standards Institute (CLSI) (CLSI, 2013 , 2019 ). Briefly, 0.5 McFarland inoculum suspensions were diluted 1:100 to a final inoculum density of 10⁵ CFU/ml. Ampicillin (AMP), ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZM), tetracycline (TET), doxycycline (DOX), ciprofloxacin (CIP), enrofloxacin (ENR), gentamicin (GEN), sulfamethoxazole (SMZ), fosfomycin (FOS), florfenicol (FFC), colistin (COL), and meropenem (MEM) (Solarbio, Beijing, China) were selected to determine the minimum inhibitory concentrations (MICs) of MPECs. The antimicrobials were twofold serially diluted ranging from 0.25 to 512 μg/ml and incubated at 37°C for 18 h. The MICs were recorded as the lowest concentrations of antimicrobials in the wells, where no visible bacteria growth was observed.

Liu Z., Liu Y., Xi W., Liu S., Liu J., Mu H., Chen B., He H., Fan Y., Ma W., Zhang W., Fu M., Wang J, & Song X. (2021). Genetic Features of Plasmid- and Chromosome-Mediated mcr-1 in Escherichia coli Isolates From Animal Organs With Lesions. Frontiers in Microbiology, 12, 707332.

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