Akt sirna 6211

Manufactured by Cell Signaling Technology

AKT siRNA (#6211) is a small interfering RNA (siRNA) that targets the AKT gene, also known as protein kinase B. The AKT gene plays a crucial role in cellular processes such as cell growth, proliferation, and survival. This product is designed to reduce the expression of the AKT gene, allowing researchers to study its biological functions and role in various cellular pathways.

Automatically generated - may contain errors

Lab products found in correlation

Anti gapdh ab181602, by Abcam (1 mentions) Control sirna 6568, by Cell Signaling Technology (1 mentions) Control sirna, by Cell Signaling Technology (1 mentions) Phosphorylated gsk 3βser9, by Cell Signaling Technology (1 mentions) Gapdh ab181602, by Abcam (1 mentions)

2 protocols using akt sirna 6211

Quantifying Protein Expression in Gastric Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

The methods were described as we previously described [13 (link)]. Anti-RPL6 (ab126100) were purchased from Cell Signaling Technology. Anti-GAPDH (ab181602) antibodies were purchased from Abcam. AKT siRNA (#6211) and control siRNA (#6568) were purchased from Cell Signaling Technology. We corrected the loading error based on loading controls and compared the expression levels of target proteins in gastric tumor and adjacent nontumor tissues. The protein expression in tumors was defined as high level when it was higher than that in normal tissue but was defined as low level when it was lower than that in normal tissue.

Lu J., Zhang P.Y., Xie J.W., Wang J.B., Lin J.X., Chen Q.Y., Cao L.L., Li P., Zheng C.H, & Huang C.M. (2019). Circular RNA hsa_circ_0006848 Related to Ribosomal Protein L6 Acts as a Novel Biomarker for Early Gastric Cancer. Disease Markers, 2019, 3863458.

+ Open protocol

+ Expand

Comparative Protein Expression Analysis in Gastric Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

The methods were described as previously described [3 (link)]. Anti-GSK-3β (#9832), AKT (pan) (#4685), phosphorylated AKT (Ser473) (#4060), GSK-3β (Ser9) (#12456) and phosphorylated GSK-3β (Ser9) (#5558) were purchased from Cell Signaling Technology. Anti-CDK5RAP3 (ab157203), E-cadherin (ab1416), N-cadherin (ab76057), Vimentin (ab8978), and GAPDH (ab181602) antibodies were purchased from Abcam. AKT siRNA (#6211) and control siRNA (#6568) were purchased from Cell Signaling Technology. We corrected the loading error based on loading controls and compared the expression levels of target proteins in gastric tumor and adjacent non-tumor tissues. The protein expression in tumors was defined as high level when it was higher than that in normal tissue but was defined as low level when it was lower than that in normal tissue.

Zheng C.H., Wang J.B., Lin M.Q., Zhang P.Y., Liu L.C., Lin J.X., Lu J., Chen Q.Y., Cao L.L., Lin M., Tu R.H., Xie J.W., Li P, & Huang C.M. (2018). CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 37, 59.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!