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Ethylenediaminetetraacetic acid

Sourced in China

Ethylenediaminetetraacetic acid (EDTA) is a chelating agent used in various laboratory applications. It has the chemical formula C10H16N2O8. EDTA is capable of forming stable complexes with metal ions, which is its core function.

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7 protocols using ethylenediaminetetraacetic acid

1

Enzymatic Assay for Lipase Activity

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RA (90 %, HPLC) was bought from Yuanye Biotechnology Co., Ltd. (Shanghai, China), p-nitrophenyl palmitate (4-NPB) was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), p-nitrophenol, l-ascorbic acid, petroleum ether, isooctane, isopropanol, disodium hydrogen phosphate dodechydrate, sodium phosphate dihydrate, ethylenediaminetetraacetic acid (EDTA), trichloroacetic acid Linoleic acid were purchased from Macklin biochemical Co., Ltd. (Shanghai, China), and all chemicals were of analytical grade.
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2

Oligonucleotide-Based Electrochemical Biosensor

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All oligonucleotides used in this study were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The oligonucleotide sequences are described in Table 1. NH4F, MgCl2, and ethylene diamine tetraacetic acid (EDTA) were procured from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). HAuCl4·4H2O was obtained from Kema Biochemical (Tianjin, China). 6-Mercapto-1-hexanol (MCH), tris (2-carboxyethy) phosphine hydrochloride (TCEP), and tris (hydroxymethyl) methyl aminomethane (Tris-base) were obtained from Sigma–Aldrich (St. Louis, USA). Ni(NO3)2·6H2O, Fe(NO3)3·9H2O, CO(NH2)2, Na2HPO4, and NaH2PO4 and NaCl were procured from Guangzhou Chemical Reagent Factory (Guangzhou, China). SnO2 transparent conductive glass (FTO, 1 cm × 2 cm) was purchased from Yingkou New Energy Technology (Yingkou, China). Double distilled water was utilized during the experiment. All chemicals were of analytical reagent grade and used directly after purchase.
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3

SARS-CoV-2 Spike Protein Binding Assay

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and ethanolamine hydrochloride were acquired from Sigma (St. Louis, MO, USA). K2HPO4, KH2PO4, NaCl, NaOH, NiCl2, ethylenediaminetetraacetic acid (EDTA), Tween-20 and heparin were obtained from Macklin Biochemical Technology (Shanghai, China). His-tagged SARS-CoV-2 S1 protein and its polyclonal antibody (anti-SARS-CoV-2, 95% purity, type rabbit) were purchased from Sangon Biotech (Shanghai, China). CM-dextran sensor chips were obtained from Biosensing Instrument Inc. (Tempe, AZ, USA). Tris-NTA sensor was prepared as described previously [49 (link)]. Other reagents were of analytical purity and used as received. Deionized water was purified by a Millipore system (Simplicity 185, Millipore Corp, Billerica, MA).
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4

Synthesis of Polyaniline Nanocomposites

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CoSO4·7H2O, FeCl3·6H2O, ethylene glycol (EG), pyrrole (Py), rhodamine B (RhB), hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl) were obtained from Aladdin (China). Sodium dodecyl sulfate (SDS), sodium acetate anhydrous (NaAc, AR), polyethylene glycol (PEG), ethylenediaminetetraacetic acid (EDTA), methanol (MA) and benzoquinone (BQ) were purchased from Macklin (China). All chemicals were used without further purification.
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5

Sturgeon Transport and Chemical Reagents

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The sturgeon was transported from Quzhou Sturgeon Aquatic Food Technology Development Co. Ltd. (Zhejiang, China) within 2 h at 4 °C. 4-hydroxy Nonenal (HNE) was purchased from GlpbBio (Shanghai, China), sodium phosphate Dibasic dodecahydrate, 1,1,3,3-Tetramethoxypropane (TMP)sodium phosphate, monobasic dehydrate, sodium chloride, ethylenediaminetetraacetic acid (EDTA), brilliant blue G, sodium hydroxide, sodium 8-Anilino-1-naphthalenesulfonate (ANS-Na), 6-FITC were bought from Macklin biochemical Co. Ltd. (Shanghai, China). All chemicals were of analytical grade.
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6

Chemical Mechanical Polishing Solution Formulation

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The solutions in this work were prepared with deionized water, silica abrasives (40% purity; JinWei Group Co. Ltd., China) and analytically pure grade chemicals, in which silica abrasives were only used for CMP experiments and Zeta potential measurements. Chemicals including hydrogen peroxide (H2O2; 30% purity), ethylenediamine (C2H8N2; 99.5% purity), ethylenediamine tetraacetic acid (C10H16N2O8; 98% purity), glycine (C2H5NO2; 99% purity), potassium citrate (C6H5K3O7; 99.3% purity), 5-methyl-benzotriazole (C7H7N3, 99.9% purity), sodium dodecyl benzene sulfonate (C12H25SO3Na, 99.97% purity) and nitric acid (as pH adjuster) were obtained from Shanghai Macklin Biochemical Technology Co. Ltd while H2O2 purchased from Jiangtian Chemical Co. Ltd., Tianjin, China.
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7

Synthesis of Metal Oxide Nanostructures

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Polyethyleneimine solution (PEI solution, average Mn ~ 60,000 by GPC, 50 wt% in H2O), zinc nitrate hexahydrate (Zn (NO3)2·6H2O, 98%) and titanium (IV) bis (ammonium lacato) dihydroxide solution (TALH solution, 50 wt% in H2O), gallium nitrate octahydrate (Ga(NO3)3·8H2O, 99.9%) were purchased from Sigma-Aldrich. copper nitrate trihydrate (Cu(NO3)2·3H2O, 99%) was purchased from Adamas-beta. yttrium nitrate hexahydrate (Y(NO3)3·6H2O, 99.9%), indium nitrate (In(NO3)3·4H2O, 99.9%), barium nitrate (Ba(NO3)2, 99%), strontium nitrate (Sr(NO3)2, 99%), ethylene diamine tetraacetic acid (EDTA, 99.9%) and anhydrous D-glucose (99%) were purchased from Macklin Inc. Crystal bond™ 509 was purchased from Structure Probe, Inc.
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