Anti cd94 clone hp 3b1

Manufactured by Beckman Coulter

The Anti-CD94 (clone HP-3B1) is a laboratory reagent used for the detection of CD94 antigen expression. CD94 is a type II transmembrane glycoprotein expressed on natural killer (NK) cells and a subset of T cells. This monoclonal antibody can be used in flow cytometry applications to identify and quantify CD94-positive cells within a sample.

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Lab products found in correlation

Cd27 fitc, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Docosahexaenoic acid, by Merck Group (1 mentions) Nervonic acid, by Merck Group (1 mentions) Lignoceric acid, by Merck Group (1 mentions) Heptadecanoic acid, by Merck Group (1 mentions) Pe cy7, by BD (1 mentions) Anti cd161 hp 3g10, by BioLegend (1 mentions) Anti nkg2d clone 149810, by R&D Systems (1 mentions)

2 protocols using anti cd94 clone hp 3b1

Quantifying HLA-E-restricted T cells

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HLA-E tetramers bound to the leader peptide derived from HLA-C*03/UL40 (sequence VMAPRTLIL, HLA-E^VMAPRTLIL) or HLA-G (VMAPRTLFL, HLA-E^VMAPRTLFL) were generated as described previously [20 (link)]. Frozen PBMC samples were thawed rapidly and checked for cell viability with trypan blue exclusion cell counting. Each sample was stained with either HLA-E^VMAPRTLIL or HLA-E^VMAPRTLFL with or without blocking antibodies to CD94 (supernatant from the XA185 hybridoma in combination with anti-CD94 (clone HP-3B1 (Beckman Coulter)) in order to prevent the tetramer binding to CD94-NKG2 receptors expressed on T cells. Fluorochrome-conjugated antibodies were added: CD27-FITC (BD Biosciences), CD8β-PE (Beckman Coulter) (or CD3-PECy7 and CD8α-APC-Cy7, BD Biosciences) and CD45RA-Alexa 700 (BD Biosciences). Flow cytometric analysis was performed using a Becton Dickinson FACSCanto II. Samples were analysed using FlowJo software (Treestar, San Carlos, USA).

Sullivan L.C., Westall G.P., Widjaja J.M., Mifsud N.A., Nguyen T.H., Meehan A.C., Kotsimbos T.C, & Brooks A.G. (2015). The Presence of HLA-E-Restricted, CMV-Specific CD8+ T Cells in the Blood of Lung Transplant Recipients Correlates with Chronic Allograft Rejection. PLoS ONE, 10(8), e0135972.

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Fatty Acid and Antibody Analysis of Natural Killer Cells

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Bovine albumin fatty acid-free and the fatty acids (1) palmitic acid (C16:0), (2) stearic acid (C18:0), (3) oleic acid (C18:1), (4) arachidic acid (20:0), (5) arachidonic acid (20:4), (6) eicosapentaenoic acid (20:5), (7) dosapentanoic acid (C22:5). (8) docosahexaenoic acid (C22:6), (9) lignoceric acid (C24:0) nervonic acid (C24:1), and (10) heptadecanoic acid (C17:0), served as an internal standard, and were purchased from Sigma Aldrich.
The following antibodies were acquired from Beckman Coulter CD3-CD16/CD56+, anti- CD158a anti-KIR2DL1/S1 (clone EB6B and clone Z27.3.7), anti-CD94 (clone HP-3B1) and anti NKG2A (CD159a, clone Z199). From Becton Dickinson, were purchased following antibodies: anti-KIR3DL1 (clone DX9), anti NKp30 (clone P30-15), anti NKp46 (clone 9E2), anti CD160 (clone BY55). Anti-human anti-CD158b (anti-KIR2DL3, clone DX27), CD 158f (anti-KI2DL5, clone UP-R1), anti-NKP44 (clone P44-8), and anti CD161 (HP-3G10) were purchased from Biolegend. The anti NKG2D (Clone # 149810) was purchased from R&D Systems. The antibodies anti-CD158e1/e2 KIR3DL1 (clone REA168), anti-KIR2DS4/CD158f (clone JJC11.6) were purchased from Miltenyi Biotech. The anti-mouse Fab FITC, PE, PE-Cy7 and the isotype controls mouse IgG1, IgG2 labelled with FITC, PE or PC5 were purchased from Becton Dickinson.

De Sanctis J.B., Dumut D.C., Radzioch D, & Hajdúch M. (2020). Functionally Relevant Differences in Plasma Fatty Acid Composition and Expression of Cytotoxic and Inhibitory NK Cell Receptors between Healthy Young and Healthy Elder Adults. Nutrients, 12(12), 3641.

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