Monocrotaline was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dimethylformamide (DMF) and PITC were obtained from Fisher Scientific (Pittsburg, PA, USA). Acetonitrile, potassium carbonate, chloroform, and diethyl ether were purchased from Bio-Rad (Hercules, CA, USA). DehydroMonocrotaline was synthesized by dehydrogenation of Monocrotaline in chloroform with o-chloranil as previously described [18 (link),19 (link)]. (isotopic purity 97%) was purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Alkaline phosphatase was acquired from Roche Diagnostics Corporation (Indianapolis, IN, USA).
Chloroform
Manufactured by Bio-Rad
Sourced in United States
Chloroform is a colorless, dense liquid commonly used in laboratory settings. It has a characteristic, sweet odor. Chloroform serves as a solvent for various organic compounds and is often employed in extraction and purification processes.
Automatically generated - may contain errors
Lab products found in correlation
Monocrotaline, by Merck Group (1 mentions)
Acetonitrile, by Bio-Rad (1 mentions)
Potassium carbonate, by Bio-Rad (1 mentions)
Dimethylformamide dmf, by Thermo Fisher Scientific (1 mentions)
Alkaline phosphatase, by Roche (1 mentions)
Agarose, by Bio-Rad (1 mentions)
Isopropanol, by Bio-Rad (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Welgene (1 mentions)
Penicillin streptomycin p s, by Welgene (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Dulbecco s modified eagle s medium dmem, by Welgene (1 mentions)
2 protocols using chloroform
1
Synthesis and Characterization of Dehydromonocrotaline
2
Extraction and Analysis of Natural Plant Compounds
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The natural plant materials used in this study, namely, Helianthus annus seed, Perilla frutescens, Prunus armeniaca, Paeonia lactiflora, Morus mongolica (Bureau) C. K. Schneid bark, Angelica gigas, Sophora flavescens, Gardenia jasminoides, Saururus chinensis, Houttuynia cordata, Acanthopanax cordata, Polygala tenuifolia, and Cimicifuga racemosa, were purchased from Seoul Yangnyeongsi Market (Seoul, Republic of Korea). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin (PS), and phosphate-buffered saline (PBS) were supplied by WelGENE (Daegu, Republic of Korea). Ham’s Nutrient Mixture F-12 (F-12) and trypsin-EDTA were obtained from Gibco-BRL (Rockville, MD, USA). Trypan blue was purchased from Sigma-Aldrich (St. Louis, MO, USA). TRIzol was obtained from Invitrogen (Carlsbad, CA, USA). Dimethyl sulfoxide (DMSO), MeOH, EtOH, chloroform, isopropanol, agarose, ethidium bromide (EtBr), and Tris were purchased from Bio-Rad (Hercules, CA, USA).
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