The VMO/ASO-injected adult zebrafish were laid on their sides on a dry paper towel, and the head and gills were covered with a wet Kimwipe. The skin surface was gently wiped with a dry Kimwipe to remove the water. Using a sharp straight Noyes Scissors 4.7 (World Precision Instruments, Sarasota, FL), a lateral incision was made at the midpoint between the dorsal and ventral fins into the region of the dorsal aorta and posterior cardinal vein. A micropipette tip was used to rapidly collect 2 μl of blood welling up at the site of incision and immediately added into a 1.5 mL Eppendorf tube containing 0.5 μl of 3.8% sodium citrate. The tube was gently mixed by finger tapping and was kept on ice and used in further experiments. This procedure was approved by the Institutional Animal Care and Use Committee of the University of North Texas, and the animal experiments were performed in compliance with institutional guidelines.
Noyes scissors 4
Manufactured by World Precision Instruments
The Noyes Scissors 4.7 are a pair of precision scissors designed for laboratory use. They feature stainless steel blades and a comfortable grip. The scissors have an overall length of 4.7 inches.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using noyes scissors 4
1
Zebrafish Blood Collection Protocol
2
Microinjection of VMO/ASO-hybrid into Zebrafish Larvae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A glass capillary of 3 inches length, OD 1.0 mm, with no filament (World Precision Instruments, Sarasota, Florida, USA) was pulled by a vertical pipette puller (David Kopf Instruments, Tujunga, California, USA). The tip was then clipped using a sharp straight Noyes Scissors 4.7 (World Precision Instruments, Sarasota, FL), and loaded with 5 μl of the VMO/ASO-hybrid prepared as described above. Three days post fertilization (dpf) larvae were anesthetized by transferring them using a plastic transfer pipette into a 0.64 mM Tricaine (pH 7.0) and incubating it for 5 seconds. The anesthetized larvae were laid on a 1.2% agarose plate and injected intravenously into the common cardinal vein with 15 nl of VMO/ASO-hybrid using Picospritzer III (Parker Precision Fluidics, Hollis, NH) and a micromanipulator under a dissection microscope. Following the injection, the larvae were transferred to E3 embryonic medium in a plastic cup at 28°C and incubated for 48 hours for use in laser-induced arterial thrombosis assay.
3
Zebrafish Blood Collection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The VMO/ASO-injected adult zebrafish were laid on their sides on a dry paper towel, and the head and gills were covered with a wet Kimwipe. The skin surface was gently wiped with a dry Kimwipe to remove the water. Using a sharp straight Noyes Scissors 4.7 (World Precision Instruments, Sarasota, FL), a lateral incision was made at the midpoint between the dorsal and ventral fins into the region of the dorsal aorta and posterior cardinal vein. A micropipette tip was used to rapidly collect 2 µl of blood welling up at the site of incision and immediately added into a 1.5 mL Eppendorf tube containing 0.5 µl of 3.8% sodium citrate. The tube was gently mixed by finger tapping and was kept on ice and used in further experiments. This procedure was approved by the Institutional Animal Care and Use Committee of the University of North Texas, and the animal experiments were performed in compliance with institutional guidelines.
4
Microinjection of VMO/ASO-Hybrid in Zebrafish
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A glass capillary of 3 inches length, OD 1.0 mm, with no filament (World Precision Instruments, Sarasota, Florida, USA) was pulled by a vertical pipette puller (David Kopf Instruments, Tujunga, California, USA). The tip was then clipped using a sharp straight Noyes Scissors 4.7 (World Precision Instruments, Sarasota, FL), and loaded with 5 µl of the VMO/ASO-hybrid prepared as described above. Three days post fertilization (dpf) larvae were anesthetized by transferring them using a plastic transfer pipette into a 0.64 mM Tricaine (pH 7.0) and incubating it for 5 seconds. The anesthetized larvae were laid on a 1.2% agarose plate and injected intravenously into the common cardinal vein with 15 nl of VMO/ASO-hybrid using Picospritzer III (Parker Precision Fluidics, Hollis, NH) and a micromanipulator under a dissection microscope. Following the injection, the larvae were transferred to E3 embryonic medium in a plastic cup at 28 °C and incubated for 48 hours for use in laser-induced arterial thrombosis assay.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!