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Anti acetyl histone h3 17 615

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Anti-Acetyl-Histone H3 (17–615) is a laboratory reagent used in research applications. It is an antibody that specifically recognizes acetylated histone H3 protein, which plays a role in chromatin regulation and gene expression. This product can be utilized in various experimental techniques, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation, to study histone acetylation patterns and dynamics.

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Lab products found in correlation

Ez chip chromatin immunoprecipitation kit, by Merck Group (2 mentions) Normal rabbit igg, by Merck Group (2 mentions)

Spelling variants of this product

Histone H3 (77 citations) Anti-acetyl-histone H3 (61 citations) Anti-histone H3 (60 citations) Acetyl-histone H3 (42 citations) Anti-H3 (36 citations) Anti-acetyl H3 (15 citations) Histones (7 citations)

2 protocols using anti acetyl histone h3 17 615

1

Chromatin Immunoprecipitation and qPCR Analysis

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ChIP was performed using EZ ChIP chromatin immuno-precipitation kit (Millipore) followed by qPCR analysis. 1 × 106 cells were stimulated, fixed, sonicated, and immunoprecipitated using 2 μg of ChIP grade antibodies: anti-BATF (sc-100974), anti-BATF3 (sc-162246), normal mouse IgG (sc-2025), anti-IRF4 (sc-6059), normal goat IgG (sc-2028) (all Santa Cruz Biotechnology), anti-Acetyl-Histone H3 (17–615), and normal rabbit IgG (Millipore). Immunoprecipitated DNA was reverse-cross-linked, purified using spin columns, and analyzed by qPCR (Primer Sequences: Supplementary Table 7). To quantify immunoprecipitated DNA, we generated a standard curve from serial dilutions of input DNA. Data are presented as percentage of input DNA based on normalization against the amount of input DNA.
Tsuda M., Hamade H., Thomas L.S., Salumbides B.C., Potdar A.A., Wong M.H., Nunnelee J.S., Stamps J.T., Neutzsky-Wulff A.V., Barrett R.J., Wang Y., Tang J., Funari V.A., Targan S.R, & Michelsen K.S. (2019). A role for BATF3 in TH9 differentiation and T cell driven mucosal pathologies. Mucosal immunology, 12(3), 644-655.
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2

Chromatin Immunoprecipitation and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP was performed using EZ ChIP chromatin immuno-precipitation kit (Millipore) followed by qPCR analysis. 1 × 106 cells were stimulated, fixed, sonicated, and immunoprecipitated using 2 μg of ChIP grade antibodies: anti-BATF (sc-100974), anti-BATF3 (sc-162246), normal mouse IgG (sc-2025), anti-IRF4 (sc-6059), normal goat IgG (sc-2028) (all Santa Cruz Biotechnology), anti-Acetyl-Histone H3 (17–615), and normal rabbit IgG (Millipore). Immunoprecipitated DNA was reverse-cross-linked, purified using spin columns, and analyzed by qPCR (Primer Sequences: Supplementary Table 7). To quantify immunoprecipitated DNA, we generated a standard curve from serial dilutions of input DNA. Data are presented as percentage of input DNA based on normalization against the amount of input DNA.
Tsuda M., Hamade H., Thomas L.S., Salumbides B.C., Potdar A.A., Wong M.H., Nunnelee J.S., Stamps J.T., Neutzsky-Wulff A.V., Barrett R.J., Wang Y., Tang J., Funari V.A., Targan S.R, & Michelsen K.S. (2019). A role for BATF3 in TH9 differentiation and T cell driven mucosal pathologies. Mucosal immunology, 12(3), 644-655.
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