Api id32

Manufactured by bioMérieux

Sourced in Italy, France, Germany

The API®/ID32 is a microbial identification system used in clinical laboratories. It is designed to identify a wide range of microorganisms, including bacteria and yeasts, through biochemical testing. The system provides a standardized and automated approach to microbial identification, allowing for accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Microflex lt mass spectrometer, by Bruker (1 mentions) Vitek ms, by bioMérieux (1 mentions) Tryptic soy broth tsb, by BD (1 mentions) Baa 47, by American Type Culture Collection (1 mentions) Malt extract agar, by Liofilchem (1 mentions) Api 50ch, by bioMérieux (1 mentions) Api 20e, by bioMérieux (1 mentions) Api zym, by bioMérieux (1 mentions)

Spelling variants of this product

API/ID32 GN (3 citations) API/ID32 phenotyping kits (3 citations) API-20E and ID32 GN Systems (2 citations) API ID32 STAPH test (2 citations)

6 protocols using api id32

Fungal Identification: Phenotypic and Molecular

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before initiating the procedure, the collection area was properly cleaned followed by antisepsis with 70% ethanol. Samples were collected during the dry and rainy seasons at intervals of 7 to 10 days. Posteriorly, the samples were transported to the Research Laboratory of the University Center UNINOVAFAPI – Piauí and to the Mycology Nucleus of the Adolfo Lutz Institute - São Paulo, for phenotypic identification and biochemical tests, respectively. The phenotypic identification was carried out according to to the identification keys described by (4 –6 ). For the biochemical test was used the commercial system API^®/ID32 (bioMérieux, Fr), following the manufacturer’s instructions.
The analysis per MALDI-TOF MS (VITEK MS, bio Mérieux, France) was carried out at the Mycology Nucleus of the Adolfo Lutz Institute - São Paulo, using a Microflex LT Mass spectrometer (Bruker Daltonik GmbH) (7 (link)). When there was a discrepancy between the results obtained by MALDI-TOF MS and by conventional identification, PCR was used with specific primers with starting sequences indicated in literature (8 ).

MOBIN M., SZESZS M.W., TAKAHASHI J.P., MARTINS M., de HIPPÓLITO D.D., PORTO J.C., TELES J.B., de LIMA S.G, & MELHEM M.D. (2018). Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil. Iranian Journal of Public Health, 47(12), 1816-1821.

+ Open protocol

+ Expand

Biofilm Formation of Clinically Relevant Bacteria

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

In these experiments, P. aeruginosa PAO1 (ATCC^® BAA-47™) and S. aureus (ATCC^® 25923) were used as reference strains. In addition, saliva from 3 volunteers without active caries or periodontal disease was used to isolate salivary bacteria. In particular, Streptococcus spp., Bacteroides fragilis, and Staphylococcus epidermidis were identified by biochemical assays (API^®/ID32, bioMérieux, Grassina, Italy) and grown at 37°C in non-selective nutrient broth (NB; Oxoid, Basingstoke, Hants, UK). The biofilm was developed, as previously described, on sterile (gamma-irradiated) specimens in a 96-well polystyrene plate with some modifications [32 (link)]. Briefly, an overnight culture of each bacterial strain or inoculum was diluted with tryptic soy broth (TSB; BD Bioscience, Milan, Italy) to an OD_{600 nm} of 0.2, and then incubated statically at 37°C in a humid atmosphere for 48–72 hours until a mature biofilm was obtained.

Di Salle A., Spagnuolo G., Conte R., Procino A., Peluso G, & Rengo C. (2018). Effects of various prophylactic procedures on titanium surfaces and biofilm formation. Journal of Periodontal & Implant Science, 48(6), 373-382.

+ Open protocol

+ Expand

Identification of Filamentous Fungi and Yeasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

After sub-isolation onto specific media such as malt extract agar (Liofilchem, Roseto degli Abruzzi, Italy) and Czapek yeast extract agar (Liofilchem, Roseto degli Abruzzi, Italy) genus and species of the filamentous fungi isolates were identified based on their macroscopic and microscopic morphological features. In accordance with the methods described by de Hoog [53 ], the macroscopic examination was based on visual observation of morphological characteristics and color of aerial mycelium, while the microscopic analysis was performed by preparation of lactophenol cotton blue-stained slides. The slides were prepared with tape that adhered to aerial mycelium and placed on the lactophenol cotton blue-stained slides. Yeasts were identified by a semi-automated sugar assimilation system API ID32 (Biomérieux Italia S.p.A., Bagno a Ripoli, Italy).

Caggiano G., Diella G., Triggiano F., Bartolomeo N., Apollonio F., Campanale C., Lopuzzo M, & Montagna M.T. (2020). Occurrence of Fungi in the Potable Water of Hospitals: A Public Health Threat. Pathogens, 9(10), 783.

+ Open protocol

+ Expand

Yeast Identification Using Multimodal Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols

Yeasts included in this study were identified by using three unbiased methods: biochemical (API^®/ID32, Biomerieux, Marcy l’Etoile, France), MALDI TOF/MS (Jagiellonian Center of Innovation, Kraków, Poland), and rDNA sequence-based identification.

Ziuzia P., Janiec Z., Wróbel-Kwiatkowska M., Lazar Z, & Rakicka-Pustułka M. (2023). Honey’s Yeast—New Source of Valuable Species for Industrial Applications. International Journal of Molecular Sciences, 24(9), 7889.

+ Open protocol

+ Expand

Physiological and Biochemical Characterization of Pathogen MCI001

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard methods were followed for the physiological and biochemical tests (32 , 33 ). Classification and identification of the strain was resolved based on Bergey’s Manual of Systematics of Archaea and Bacteria (34 (link)). Vitamin biosynthesis was analyzed using microbiological assay methods (35 (link)). For phenotypic characterization of MCI001, commercially available API kits were used [API 50 CH, API 20E, API ID 32 and API ZYM; bioMe´rieux, Germany]. In all experiments, type strain P. histicola DSM 19854 and P. melaninogenica ATCC 25845 were used as controls. Colony morphology was observed by culturing microbes on tryptic soy agar [TSA] and tryptic soy blood agar plates. For broth culture, tryptic soy broth [TSB] was used. The culture conditions were kept at 37°C for 24 – 96 hours, with pH of 7.2 ± 0.2. Susceptibility to antimicrobial agents was tested using commercially prepared antimicrobial discs [BD Life Sciences, USA]. For electron microscopy, bacteria cultured for 36 hrs. in TSB were collected through centrifugation followed by washing with PBS and suspended in Trump’s fixative for slide preparation. Transmission Electron Microscopy [TEM] was done using a Philips CEM 300 electron microscope at Electron Microscopy Facility at Mayo Clinic, Rochester, USA.

Balakrishnan B., Luckey D., Bodhke R., Chen J., Marietta E., Jeraldo P., Murray J, & Taneja V. (2021). Prevotella histicola Protects From Arthritis by Expansion of Allobaculum and Augmenting Butyrate Production in Humanized Mice. Frontiers in Immunology, 12, 609644.

+ Open protocol

+ Expand

Salmonella Serotyping and Resistance Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serotyping according to White-Kauffmann-Le Minor scheme [8] was performed to verify Salmonella serovar. Biochemical properties of isolates were evaluated by API ID32 ® , performed and coded according to manufacturer's instruction (bioMérieux Polska, Warsaw, Poland).
MIC testing and identification of selected resistance mechanisms. Microbiological resistance was assessed by the microbroth dilution method. Minimal Inhibitory Concentration (MIC) of 14 antimicrobials (Fig. 1) were interpreted according to the epidemiological cut-off values (http://www.eucast.org). Etests® (ESBL and AmpC; AB bioMerieux, Solna, Sweden) were performed for phenotypic confirmation of cephalosporin resistance and differentiation between extended spectrum betalactamase and AmpC cephalosporinase production. Resistance mechanism was identified with bla CMY-2 PCR amplification [9] . Isolate showing wild-type nalidixic acid MIC and microbiological resistance to ciprofloxacin was tested with PCR for qnrS amplicon [10] , and further sequenced for resistance mechanism identification (Oligo, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland). Both PCR assays were performed with Maxima ® Hot Start PCR Master Mix according to manufacturer's recommendation (Fermentas Life Sciences, Thermo Scientific, Lithuania).

Hoszowski A., Zając M., Lalak A., Przemyk P, & Wasyl D. (2016). Fifteen years of successful spread of Salmonella enterica serovar Mbandaka clone ST413 in Poland and its public health consequences. Annals of agricultural and environmental medicine : AAEM, 23(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!