PBMC were labeled for 40 minutes at room temperature with 4 cocktails of fluorescently conjugated antibodies (targets, clones, and suppliers are listed in Table E1 ) in PBS 1% BSA, washed in PBS 1% BSA and then PBS alone, and stained with live/dead fix aqua (Invitrogen). Stained cells were fixed in 4% paraformaldehyde (ThermoFisher) and analyzed using a LSRII (BD Bioscience) or iQue3 (Sartorius) flow cytometer. Data were gated using FlowJo version 10 (BD Bioscience) or ForeCyt version 7.02 (Sartorius). Gating strategies are shown in Fig. E7 .
Live dead fix aqua
Manufactured by Thermo Fisher Scientific
The Live/Dead Fix Aqua is a laboratory instrument designed for the determination of cell viability. It provides a rapid and accurate assessment of live and dead cells in a sample. The device utilizes fluorescent dyes to distinguish between living and non-viable cells, allowing for the quantification of cell populations.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using live dead fix aqua
1
Multicolor Flow Cytometry of PBMCs
2
Flow Cytometry Analysis of PBMCs
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Fresh PBMCs were labeled for 40 minutes with fluorescently conjugated antibodies (targets and clones listed in Table E1 ) in phosphate buffered saline (PBS), 1% bovine serum albumin (BSA) (Invitrogen), washed, stained for viability with live-dead fix aqua (Invitrogen) in PBS (Sigma) and fixed in 4% paraformaldehyde (ThermoFisher). Fixed cells were analyzed using an LSRII flow cytometer (BD Bioscience) running Diva software (BD Bioscience) and data gated using FlowJo software (BD Bioscience) (Fig. E1 ). Baseline data were collected 5 months before irradiation.
3
Multicolor Flow Cytometry of PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PBMC were labeled for 40 minutes at room temperature with 4 cocktails of fluorescently conjugated antibodies (targets, clones, and suppliers are listed in Table E1 ) in PBS 1% BSA, washed in PBS 1% BSA and then PBS alone, and stained with live/dead fix aqua (Invitrogen). Stained cells were fixed in 4% paraformaldehyde (ThermoFisher) and analyzed using a LSRII (BD Bioscience) or iQue3 (Sartorius) flow cytometer. Data were gated using FlowJo version 10 (BD Bioscience) or ForeCyt version 7.02 (Sartorius). Gating strategies are shown in Fig. E7 .
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