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Anti mouse rabbit hrp linked secondary antibody

Manufactured by Cell Signaling Technology
Sourced in United States

The Anti-mouse/rabbit HRP-linked secondary antibody is a laboratory reagent used in immunoassays and Western blotting to detect the presence of mouse or rabbit primary antibodies. The antibody is conjugated with horseradish peroxidase (HRP), an enzyme that catalyzes a colorimetric or chemiluminescent reaction, allowing for the visualization and quantification of the target protein.

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2 protocols using anti mouse rabbit hrp linked secondary antibody

1

Protein Expression and Oxidative Stress

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Primary antibodies used in this study included mouse anti-TH (Millipore, Temecula, CA, MAB318), rabbit anti-GAPDH (Cell Signaling, Danvers, MA, 2118), rabbit anti-4-HNE (Alpha diagnostics, San Antonio, TX, HNE11-S), rabbit anti-HO-1 (Millipore, Temecula, CA, AB1284), rabbit anti-pJNK (Cell Signaling, Danvers, MA, 9251), rabbit anti-JNK (Cell Signaling, Danvers, MA, 9252). Secondary antibodies used in this study included anti-mouse/rabbit HRP-linked secondary antibody (Cell Signaling, Danvers, MA, 7076 or 7074), Alexa Fluor 488 donkey anti-mouse secondary antibody (Life technologies, Eugene, OR, A21202), Alexa Fluor 568 donkey anti-rabbit secondary antibody (Life technologies, Eugene, OR, A10042), goat anti-mouse or goat anti-rabbit (Millipore, Temecula, CA, AP124 or AP132), and mouse or rabbit PAP antibody (Jackson Immunoresearch, West Grove, PA). PQ dichloride hydrate (Sigma, St. Louis, MO, 856177) was purchased and dissolved in saline for mice treatment.
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2

Immunohistochemical Analysis of MPTP-Induced Parkinson's

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The primary antibodies used in this study included mouse anti-TH (Millipore, Temecula, CA, USA, MAB318), rabbit anti-GAPDH (Cell Signaling, Danvers, MA, USA, 2118), rabbit anti-4-HNE (Alpha Diagnostics, San Antonio, TX, USA, HNE11-S), GFAP (Invitrogen, Rockford, IL, USA, PA5-16291), and iba1 (Wako, Osaka, Japan, 019-19741). Secondary antibodies used in this study included anti-mouse/rabbit HRP-linked secondary antibody (Cell Signaling, Danvers, MA, USA, 7076 and 7074) and goat anti-mouse or goat anti-rabbit (Millipore, Temecula, CA, USA, AP124 or AP132) peroxidase-conjugated antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride (Santa Cruz, CA, USA, 23007-85-4) was purchased and dissolved in saline for mice treatment.
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