Passage 3 WJ-MSCs were trypsinized, dissociated into a single cell suspension, and allowed to reach 60% confluency. Cells were then rinsed with phosphate buffered saline (PBS) and incubated with anti-human CD73-PE (BioLegend, 344,004), anti-human CD105-PE (BioLegend, 323,206), anti-human CD90-PE (BioLegend, 328,110), anti-human CD34-PE (BioLegend, 343,506), and anti-human CD45-FITC (BioLegend, 304,006) for 15 min at room temperature. After incubation, the cells were rinsed with PBS, read on a FACSCalibur (BD, USA) flow cytometer, and analyzed using the WinMDI 2.8 software. Mouse IgG1-PE (BioLegend, 400,114) and mouse IgG1-FITC (BioLegend, 400,107) were used as the isotype controls.
Anti human cd34 pe
Manufactured by BioLegend
Sourced in United States
Anti-human CD34-PE is a fluorochrome-conjugated monoclonal antibody that binds to the CD34 antigen, a glycoprotein expressed on the surface of hematopoietic stem and progenitor cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti human cd11b pe, by BD (2 mentions)
Anti human cd19 pe cy7, by BioLegend (2 mentions)
Anti mouse cd16 cd32, by BioLegend (2 mentions)
Rbc lysis buffer, by Santa Cruz Biotechnology (2 mentions)
Anti mouse cd45 percp cy5, by BioLegend (2 mentions)
Anti human cd45 apc, by BD (2 mentions)
Anti human cd3 pe dazzle 594, by BioLegend (2 mentions)
Anti mouse cd45 percp, by BD (2 mentions)
Facscalibur, by BD (1 mentions)
Fitc mouse igg1, by BioLegend (1 mentions)
Pe mouse igg1, by BioLegend (1 mentions)
Pe anti human cd105, by BioLegend (1 mentions)
Anti human cd90 pe, by BioLegend (1 mentions)
Fitc anti human cd45, by BioLegend (1 mentions)
Facscanto, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Anti human cd235a apc, by Miltenyi Biotec (1 mentions)
4 protocols using anti human cd34 pe
1
Flow Cytometric Immunophenotyping of MSCs
2
Multiparameter Flow Cytometry of Hematopoietic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
50 μL peripheral blood or ~5×105 bone marrow cells in PBS with 10% FBS were blocked with anti-mouse CD16/CD32 (cat # 101302, Biolegend, San Diego, CA, USA) and then stained with fluorophore conjugated antibodies. Following staining, red blood cells were lysed with RBC lysis buffer (cat # sc-296258, Santa Cruz Biotechnology, Dallas, TX, USA) for 10 min and washed twice with 10% FBS in PBS. Peripheral blood samples underwent a second 5 min RBC lysis prior to washing. Samples were acquired on a BD Accuri C6 or Beckman coulter Gallios flow cytometer and analyzed using BD Accuri Csampler software or FlowJo. Cells were stained with anti-human CD34 PE (cat # 343606, Biolegend), anti-human CD11b PE (cat # 555388, BD Biosciences, San Jose, CA, USA), anti-human CD3 PE/Dazzle™ 594 (cat # 300450, Biolegend), anti-mouse CD45 PerCP-Cy5.5 (cat # 103132 Biolegend), anti-mouse CD45 PerCP (cat # 557235, BD), anti-human CD19 PE-Cy7 (cat # 302216, Biolegend), anti-human CD45 APC (cat # 555485, BD Bioscience or cat # 304012, Biolegend). Analysis based 10 000 bone marrow and 3 000 blood cells from the live cell gate.
3
Flow Cytometry Analysis of Hematopoietic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested, washed with PBS twice, and incubated with the desired antibodies for 15 min at RT in the dark. Cells were washed twice, centrifuged at 2000 rpm, and resuspended in 300 μl of 2% FBS/PBS before being subjected to FACSCanto flow cytometry (BD Bioscience) and analyzed using FACS DIVA software (BD Bioscience). The antibodies used for flow cytometry were anti-human CD34-PE (#343506; BioLegend, San Diego, CA, USA), anti-human CD235a-APC (#130-100-270; Miltenyi Biotec), and anti-human CD41-FITC (#303704; BioLegend).
4
Multiparameter Flow Cytometry of Hematopoietic Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!