Monoclonal antibodies against e cadherin

Monoclonal antibodies against Snail-1, ERK1/2, phospho-ERK1/2, phospho-SMAD1-5-8, and Cav1 were from Cell Signaling Technology; monoclonal antibodies against E-cadherin, β-catenin, and γ-catenin were from BD (Becton-Dickinson Laboratories, Mountain View, CA); monoclonal antibodies against occludin was from Invitrogen (Carlsbad, CA); monoclonal antibodies against tubulin, α-SMA, α-catenin, vimentin, pan-cytokeratin, and fibronectin were from Sigma (Saint Louis, MO); monoclonal anti-N-cadherin and polyclonal anti-ZO-1 were from Zymed (Invitrogen, Carlsbad, CA); polyclonal anti-actin, -SMAD2-3, and -phospho-MEK were from Santa Cruz Biotechnology (CA); and polyclonal anti-phospho SMAD2-3 was from Biosource (Camarillo, CA). Monoclonal anti-CD54 was from Biolegend (San Diego, CA); polyclonal anti-Fsp1 was from Dako (Glostrup, Denmark); monoclonal anti-CD31 from Serotec (Oxford, UK); and polyclonal anti-Ki67 from Abcam (Cambridge, UK). Monoclonal anti-JAM-A clone BV-11 was a gift from Dr. E. Dejana (Milan Italy). Fluor 647?phalloidin and Hoechst 33342 were from Invitrogen (Carlsbad, CA). CI 1040 was from Selleck (Houston, TX).

Monoclonal antibodies against Snail and HDAC1 (for WB experiments) were from Cell Signaling technology; polyclonal antibodies against Snail (H-130, for ChIP experiments) were from Santa Cruz Biotech (Dallas, TX); monoclonal antibodies against E-cadherin were from BD (Becton-Dickinson Laboratories, Mountain View, CA); monoclonal antibodies against α-SMA were from Sigma (Saint Louis, MO); polyclonal anti-ZO-1 were from Zymed (Invitrogen, Carlsbad, CA); polyclonal antibodies against HDAC1 (for ChIP experiments), GAPDH, Actin, acetyl-H3 and rabbit IgG control were from Millipore (Merck, Kenilworth, NJ). Matrigel Matrix Growth Factor Reduced (GFR) was from BD bioscences (Milan, Italy). Magnetic beads (Dynabeads) were from Invitrogen (Carlsbad, CA).
Rhodamine-phalloidin and Hoechst 33342 were from Invitrogen. TSA was from Selleck (Houston, TX), MC1568, MS-275, MC2500 were from Mai lab. Stay safe balance 4.25% PD fluid was from Fresenius (Bad Homburg, Germany).

Dulbecco's modified Eagle's medium (DMEM) was from Life Technologies Inc. and fetal calf serum from Lonza. Gentamicin, mowiol, paraformaldehyde, mammalian protease and phosphatase inhibitor cocktails were from Sigma France. The soluble recombinant human NTSR3/sortilin protein (Ser78-Asn755; MW = 76 kDa from R&D systems) was resuspended in PBS containing 0.1% bovine serum albumin (BSA) as a carrier following the manufacturer's recommendations. All control conditions were made using PBS containing 0.1% BSA. Monoclonal antibodies against E-cadherin and NTSR3/sortilin were from BD Bioscience. Alexa-594-phalloidin was from Invitrogen. Alexa-488 conjugated donkey anti-rabbit and Alexa-594 conjugated donkey anti-mouse antibodies were obtained from Jackson Immunoresearch Laboratories. HRP conjugated goat anti-rabbit and anti-mouse were from Cell Signalling.