Fourier transform infrared (FT-IR) spectroscopy of native curcumin and nanotized curcumin was performed on a FT-IR spectrophotometer (Spectrum 100 optica; PerkinElmer Inc., Waltham, MA, USA). The pellets of sample (10 mg) and potassium bromide (200 mg) were prepared by compressing the powders at 5 bars for 5 minutes on a KBr press and the spectra were scanned on the wavenumber range of 4,000–450 cm−1.
Spectrum 100 optica
Manufactured by PerkinElmer
Sourced in United States
The Spectrum 100 Optica is a versatile laboratory instrument designed for a wide range of spectroscopic applications. It features a high-performance optical system that enables accurate and reliable measurements across a broad range of wavelengths.
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Lab products found in correlation
6 protocols using spectrum 100 optica
1
FT-IR Spectroscopy of Curcumin Nanoformulations
2
Bone Tissue Analysis by FT-IR Spectroscopy
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Tibiae of male mice were fixed in 70% EtOH and embedded in Osteoresin, and sections (4 μm in thickness) were prepared on BaF2 slides (Pier-Optics, Tatebayashi, Japan). FT-IR spectra were obtained using the Spectrum 100 Optica (PerkinElmer, Waltham, MA, USA) and Spotlight 400 (PerkinElmer, Waltham, MA, USA). The microscope was equipped with a computer-controlled x/y stage that permitted spectral sampling of the tissue in defined steps within a rectangular area. IR spectra were collected with an aperture diameter of 50 μm, and transmission from 4000 to 700 cm–1 with a spectral resolution of 4 cm–1 using an MCT detector. One hundred and twenty-eight scans per point were collected and averaged.
3
Comprehensive Analytical Characterization
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Infrared spectra were measured on a Perkin Elmer Spectrum 100 Optica (Perkin Elmer, Wellesley, MA, USA). 1H- and 13C-NMR spectra were recorded on a Bruker Avance III 200 spectrometer at 200 MHz and 50 MHz respectively, Bruker Avance I 300 at 300 MHz and 75 MHz respectively, on a Bruker Advance II 400 spectrometer at 400 MHz and 100 MHz respectively, on a Bruker Advance III 600 spectrometer at 600 MHz and 150 MHz respectively (Bruker, Billerica, MA, USA). Shifts (δ) are given in parts per million with respect to solvent residual peak and coupling constants (J) are given in Hertz. HRMS (FD) spectra were performed by CESAMO (Bordeaux, France). The measurements were carried out on a TOF mass spectrometer AccuTOFGCv using an FD emitter with an emitter voltage of 10 kV. One to two microliters solution of the compound is deposited on a 13 μm emitter wire. Elemental analyses were carried out by the “Institut de Chimie des Substances Naturelles” (Gif-sur-Yvette, France). Melting points were measured on Stuart SMP 10.DSC measures were carried out on Mettler Toledo DSC 1 STAReSystem (Mettler, Greifensee, Switzerland).
4
Evaluating Resin Conversion via FTIR
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The degree of resin conversion was analyzed with Fourier transform infrared spectroscopy (FTIR) (Spectrum 100 Optica, PerkinElmer; Waltham, MA, USA) with an attenuated total reflectance element (ATR) coupled to a horizontal zinc selenide crystal (Pike Technologies; Madison, WI, USA) with a standard compression force of 100 N and 32 absorbance scans for each sample. Initially, an unpolymerized resin sample (no.=6)26 (link),27 (link) from each group was assessed by positioning it on the crystal and then performing 4 absorbance scans.
The obtained infrared spectra were assessed using an intensity of 1637 cm−1 and a peak at 2952 cm−1, which correspond to the double carbon bonds between the methacrylate and the simple connections made via polymerization, respectively15 (link) as shown inFigure 1 . Using the differences in the peak heights of the polymerized and unpolymerized samples and based on the fact that polymerization occurs from linked pairs breakdown and single bonds formation. The degree of conversion was calculated according to the following formula, and the values are reported as percentages (%):
The mean values for all groups were obtained and then subjected to a two-way ANOVA and subsequent Tukey tests with the predetermined α of 0.05.
The obtained infrared spectra were assessed using an intensity of 1637 cm−1 and a peak at 2952 cm−1, which correspond to the double carbon bonds between the methacrylate and the simple connections made via polymerization, respectively15 (link) as shown in
The mean values for all groups were obtained and then subjected to a two-way ANOVA and subsequent Tukey tests with the predetermined α of 0.05.
5
FTIR-Based Measurement of Degree of Cure
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After 24 hours of storage, DC was measured for five specimens by using a Fourier transform infrared spectroscope (FTIR, Spectrum 100 Optica, PerkinElmer, Cambridge, MA, USA), equipped with an attenuated total reflectance (ATR) device with a horizontal ZnSe crystal (Pike Technologies, Madison, WI, USA). The specimens were kept in contact with the horizontal face of the ATR cell.
A preliminary reading for the uncured material was recorded under the following conditions: frequency range of 1,665 – 1,580 cm-1, 32 scans, resolution of 4 cm-1, and Happ-Genzel apodization in the absorbance mode. DC was calculated using a baseline technique based on the band ratios of 1,638 cm-1 (aliphatic carbon-to-carbon double bond) and 1,608 cm-1 (aromatic component group) as an internal standard between the polymerized and the uncured samples.22 (link)
A preliminary reading for the uncured material was recorded under the following conditions: frequency range of 1,665 – 1,580 cm-1, 32 scans, resolution of 4 cm-1, and Happ-Genzel apodization in the absorbance mode. DC was calculated using a baseline technique based on the band ratios of 1,638 cm-1 (aliphatic carbon-to-carbon double bond) and 1,608 cm-1 (aromatic component group) as an internal standard between the polymerized and the uncured samples.22 (link)
6
Evaluating Adhesive Curing Efficiency
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The experimental adhesives were dispensed directly into a bar-shaped metal matrix measuring 7 mm x 2 mm x 1 mm (Odeme Dental Research, Luzerna, Brazil). The solvents were not evaporated to enable assessing their effect on curing. A glass plate was placed on the mold to prevent solution waste, by reducing contact with atmospheric oxygen, and keeping the solvents from evaporating. The photoactivation was performed immediately using a Valo LED unit (1,200 mW/cm², Ultradent, South Jordan, USA) for 40 s. The specimens were positioned on a zinc selenide crystal (SeZn) of an attenuated total reflectance device of the FTIR spectrometer (Spectrum 100 Optica; Perkin Elmer, São Paulo, Brazil), Next, the C = C aliphatic (1,635 cm -1 ) and the aromatic (1,608 cm -1 ) peaks were registered after the baseline correction, and the degree (%) of conversion was calculated for each material. Experiments were performed in triplicate (n = 3) for each experimental adhesive. The values were subjected to one-way ANOVA statistical analysis and Tukey's test (p < 0.05).
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