ZHBTc4 cells were treated for 0 and 24 hr. Next, cells were crosslinked with 4% paraformaldehyde (Sigma-Aldrich, D6148-500G) for 30 min. Formaldehyde was quenched with 50 mM glycine (Sigma-Aldrich, G8898-1KG) for 15 min. Cells were stained for Oct4 (Santa Cruz sc-5279) or Sox2 (Santa Cruz, sc17320) overnight at 4°C. The following day samples were incubated with anti-goat-alexa-488 (Thermoscientific, A11078) or anti-goat-alexa-568 (Thermoscientific, A11061) and Hoechst (Sigma-Aldrich, H6024).
G8898 1kg
Manufactured by Merck Group
The G8898-1KG is a laboratory equipment product from Merck Group. It is a chemical reagent used for various laboratory applications. The core function of this product is to provide a standardized and high-quality chemical compound for use in research, testing, and other laboratory procedures. No further details on the intended use or specific applications of this product are provided.
Automatically generated - may contain errors
2 protocols using g8898 1kg
1
Immunofluorescence Staining of Stem Cell Markers
2
Cross-linking and TNF Stimulation for NicE-C and RNA-seq
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cross-linking, cultured cells were collected by trypsin and then fixed at room temperature for 10 min with 1% formaldehyde (Sigma-Aldrich F8775-500ML). After cross-linking, 2.5 M Glycine (Sigma-Aldrich G8898-1KG) was added to the final 125 mM and incubated at room temperature for 10 min to quench the reaction. Fixed cells were pelleted and washed twice with ice-cold 1× PBS. After removing the supernatant, the fixed cells were stored at –80°C until used. TNF (Sino biological 10602-HNAE) was dissolved in water to a final concentration of 20 ng/µL. Cultured HeLa-S3 cells were washed once with 1× PBS, and fresh DMEM media supplemented with final 10 ng/mL TNF or water were added. Cells were then incubated for 60 min and subjected to NicE-C or RNA-seq.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!