Elexsys 2 e500 cw epr spectrometer

Manufactured by Bruker

Sourced in Germany

The Elexsys-II E500 CW-EPR spectrometer is a continuous wave electron paramagnetic resonance (CW-EPR) instrument designed for research and analysis applications. The core function of the Elexsys-II E500 is to detect and measure the magnetic properties of unpaired electrons in a sample, providing information about the chemical and structural composition of the material.

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Lab products found in correlation

Itc503, by Oxford Instruments (3 mentions) Esr900 liquid helium cryostat, by Oxford Instruments (3 mentions) Xepr software, by Bruker (2 mentions) Epr quartz tubes, by Wilmad (2 mentions)

Close spelling variants of this product

Elexsys E500 CW-EPR spectrometer ELEXSYS II E500 EPR spectrometer ELEXSYS E500 EPR spectrometer E500 CW-EPR spectrometer ELEXSYS-II E500 spectrometer ELEXSYS II EPR spectrometer Elexsys E500 spectrometer E500 EPR spectrometer ELEXSYS-II E500 Elexsys E500

6 protocols using elexsys 2 e500 cw epr spectrometer

EPR Spectral Analysis of Samples

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The Electron Paramagnetic Resonance (EPR) spectra were recorded as described earlier (11) at the University of Vienna on an X-Band Bruker Elexsys-II E500 CW-EPR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) at 90 ± 1 and 293 ± 1 K using a high sensitivity cavity (SHQE1119). Solid state EPR measurements were performed setting microwave frequency to 9 GHz, modulation frequency to 100 kHz, center field to 6000 G, sweep width to 12000 G, sweep time to 335.5 s, modulation amplitude to 20.37 G, microwave power to 15 mW, conversion time to 81.92 ms, and resolution to 4096 points. The samples were put in EPR quartz tubes (Wilmad-LabGlass, Vineland, NJ, United States) and scanned three times, of which the average was used for analysis. The spectrum of an empty control tube was subtracted from all sample spectra. All spectra were analyzed with the Bruker Xepr software.

Milojevic T., Kölbl D., Ferrière L., Albu M., Kish A., Flemming R.L., Koeberl C., Blazevic A., Zebec Z., Rittmann S.K., Schleper C., Pignitter M., Somoza V., Schimak M.P, & Rupert A.N. (2019). Exploring the microbial biotransformation of extraterrestrial material on nanometer scale. Scientific Reports, 9, 18028.

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X-band EPR Spectroscopy of Samples

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Samples for X-band (~9.4 GHz) EPR spectroscopy were measured at the CalEPR center at the University of California, Davis. Continuous wave (CW) spectra were collected using a Bruker Instruments EleXsys-II E500 CW EPR spectrometer (Bruker Corporation, Billerica, MA) equipped with an Oxford Instruments ESR900 liquid helium cryostat and an Oxford Instruments ITC503 temperature and gas-flow controller. Samples were measured under non-saturating slow-passage conditions using a Super-High Q resonator (ER 4122SHQE). Typical acquisition conditions were: T = 6.5 K; 9.38 GHz microwave frequency; 10G modulation amplitude; 0.6325 mW microwave power.

Hu S., Offenbacher A.R., Thompson E.M., Gee C.L., Wilcoxen J., Carr C.A., Prigozhin D.M., Yang V., Alber T., Britt R.D., Fraser J.S, & Klinman J. (2019). Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The “Undoing” of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network. Journal of the American Chemical Society, 141(4), 1555-1567.

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EPR Analysis of Lipid Radical Formation

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The Electron paramagnetic resonance spectroscopy (EPR) measurements were performed on a Bruker Elexsys II E500-CW-EPR spectrometer (Rheinstetten, Germany) operating with an X-band at 9.85 GHz and a modulation frequency of 100 kHz at room temperature. The evaluation was performed with the Bruker-software Xepr 2.6b.52. For measurements,⁴⁹ (link) PBN solution was prepared in a 200 mM concentration with ethanol, and then added into the potential-treated peptide solution and the control peptide solution for 1 h of electrolysis with 0.3 V versus Ag/AgCl potential applied. The EPR settings for measurement of short-lived lipid radicals were as follows: microwave attenuation = 10 dB; modulation amplitude = 1.0 G; receiver gain = 80 dB; sweep width = 100 G; microwave power = 20 mW; time constant = 163.84 ms; conversion time = 100.05 ms and center field = 3511.45 G.

Yu Q., Mao H., Yang B., Zhu Y., Sun C., Zhao Z., Li Y, & Zhang Y. (2023). Electro-polarization of protein-like substances accelerates trans-cell-wall electron transfer in microbial extracellular respiration. iScience, 26(2), 106065.

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Electron Paramagnetic Resonance Spectroscopy

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The Electron Paramagnetic Resonance (EPR) spectra were recorded on an X-Band Bruker Elexsys-II E500 CW-EPR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) at 90 ± 1 and 293 ± 1 K using a high sensitivity cavity (SHQE1119). Solid state EPR measurements were performed setting microwave frequency to 9 GHz, modulation frequency to 100 kHz, center field to 6000 G, sweep width to 12000 G, sweep time to 335.5 s, modulation amplitude to 20.37 G, microwave power to 15 mW, conversion time to 81.92 ms and resolution to 4096 points. The samples were put in EPR quartz tubes (Wilmad-LabGlass, Vineland, NJ, United States) and scanned three times, of which the average was used for analysis. The spectrum of an empty control tube was subtracted from all sample spectra. All spectra were analyzed with the Bruker Xepr software.

Kölbl D., Pignitter M., Somoza V., Schimak M.P., Strbak O., Blazevic A, & Milojevic T. (2017). Exploring Fingerprints of the Extreme Thermoacidophile Metallosphaera sedula Grown on Synthetic Martian Regolith Materials as the Sole Energy Sources. Frontiers in Microbiology, 8, 1918.

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X-band EPR Spectroscopy Protocol

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Samples for X-band (~9.4 GHz) EPR spectroscopy were measured at the CalEPR center at the University of California, Davis. Continuous wave (CW) spectra were collected using a Brüker Instruments Elexsys-II E500 CW EPR spectrometer (Brüker Corporation, Billerica, MA) equipped with an Oxford Instruments ESR900 liquid helium cryostat and Oxford Instruments ITC503 temperature and gas-flow controller. Samples were measured under non-saturating slow-passage conditions using a Super-High Q resonator (ER 4122SHQE). Spectra simulations were performed using the EasySpin 4.0 toolbox in Matlab.^{44 (link)}

Bruender N.A., Wilcoxen J., Britt R.D, & Bandarian V. (2016). Biochemical and spectroscopic characterization of a radical SAM enzyme involved in the formation of a peptide thioether crosslink. Biochemistry, 55(14), 2122-2134.

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X-band EPR Spectroscopy Protocol

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Samples for X-band (~9.4 GHz) EPR spectroscopy were measured at the CalEPR center at the University of California, Davis. Continuous wave (CW) spectra were collected using a Bruker Instruments EleXsys-II E500 CW EPR spectrometer (Bruker Corporation, Billerica, MA) equipped with an Oxford Instruments ESR900 liquid helium cryostat and an Oxford Instruments ITC503 temperature and gas-flow controller. Samples were measured under non-saturating slow-passage conditions using a Super-High Q resonator (ER 4122SHQE). Typical acquisition conditions were: T= 100 K; 9.4 GHz microwave frequency; 3G modulation amplitude; 1 mW microwave power.

Wilcoxen J., Bruender N.A., Bandarian V, & Britt R.D. (2018). A Radical Intermediate in Bacillus subtilis QueE During Turnover With the Substrate Analog 6-Carboxypterin. Journal of the American Chemical Society, 140(5), 1753-1759.

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