Bacterial alkaline phosphatase type 3 escherichia coli

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Bacterial alkaline phosphatase Type III (Escherichia coli) is an enzyme derived from the bacterium Escherichia coli. It catalyzes the hydrolysis of phosphate groups from a variety of molecules, including nucleotides, proteins, and other substrates.

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Lab products found in correlation

Nuclease p1, by Fujifilm (2 mentions) Hplc grade acetonitrile, by Fujifilm (2 mentions) Phosphodiesterase 1, by Worthington (2 mentions) Bovine spleen phosphodiesterase 2, by Merck Group (2 mentions) Dnase 1, by Merck Group (2 mentions) Apocynin, by Tokyo Chemical Industry (1 mentions) 1 4 dioxane, by Fujifilm (1 mentions)

2 protocols using bacterial alkaline phosphatase type 3 escherichia coli

Reagents for DNA and RNA Analysis

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o-Toluidine hydrochloride (T536215; purity, 97%) was obtained from Toronto Research Chemicals, Toronto, ON, Canada, p-toluidine (PT) hydrochloride (T0303; purity, >98%) was obtained from Tokyo Chemical Industry, Tokyo, Japan, and aniline (ANL) hydrochloride (017-04092; purity, 97%) was obtained from Wako Pure Chemical Industries, Osaka, Japan. AAOT (A1020; purity, >98%) and apocynin (H0261; purity, >98%) were obtained from the Tokyo Chemical Industry. Nuclease P1 and HPLC-grade acetonitrile were purchased from Wako Pure Chemical Industries, Ltd. Phosphodiesterase I was purchased from Worthington Biochem (Lakewood, NJ, USA). Bovine spleen Phosphodiesterase II, DNase I, and bacterial alkaline phosphatase Type III (Escherichia coli) were purchased from SigmaAldrich (St. Louis, MO, USA). All other chemicals used were of analytical grade and purchased from Wako Pure Chemical Industries, Ltd.

Suzuki S., Gi M., Komiya M., Obikane A., Vachiraarunwong A., Fujioka M., Kakehashi A., Totsuka Y, & Wanibuchi H. (2023). Evaluation of the Mechanisms Involved in the Development of Bladder Toxicity following Exposure to Occupational Bladder Cancer Causative Chemicals Using DNA Adductome Analysis. Biomolecules, 14(1), 36.

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Nuclease P1 and Phosphodiesterase Analysis

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1,4-Dioxane was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan; purity >99.9%). Nuclease P1 and HPLC-grade acetonitrile were also purchased from Wako Pure Chemical Industries, Ltd. Phosphodiesterase I was purchased from Worthington Biochem (Lakewood, NJ, U.S.A.). Bovine spleen Phosphodiesterase II, DNase I, and bacterial alkaline phosphatase Type III (Escherichia coli) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). All other chemicals used were of analytical grade and purchased from Wako.

TOTSUKA Y., MAESAKO Y., ONO H., NAGAI M., KATO M., GI M., WANIBUCHI H., FUKUSHIMA S., SHIIZAKI K, & NAKAGAMA H. (2020). Comprehensive analysis of DNA adducts (DNA adductome analysis) in the liver of rats treated with 1,4-dioxane. Proceedings of the Japan Academy. Series B, Physical and Biological Sciences, 96(5), 180-187.

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