Log In Sign up
The largest database of trusted experimental protocols

Cd1d pe

Manufactured by BioLegend
Visit Supplier

CD1d-PE is a fluorophore-conjugated antibody that binds to the CD1d protein. CD1d is a major histocompatibility complex (MHC) class I-like molecule that presents lipid antigens to natural killer T (NKT) cells. The PE (phycoerythrin) fluorophore allows for the detection and analysis of CD1d-expressing cells using flow cytometry or other fluorescence-based techniques.

Automatically generated - may contain errors

Lab products found in correlation

Ly6g peridinin chlorophyll protein percp cy5, by BD (1 mentions) Cd45 percp cy5, by BioLegend (1 mentions) Cd4 allophycocyanin apc, by BD (1 mentions) Cd25 fitc, by BD (1 mentions) Cd45 fitc 30 f11, by BioLegend (1 mentions) Cd11b apc fire750, by BioLegend (1 mentions) Cd8a pe, by BioLegend (1 mentions) Cd5 bv421, by BioLegend (1 mentions) Facsaria instrument, by BD (1 mentions) Facsverse instrument, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Ifn γ apc, by BioLegend (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Igg1 pe, by BioLegend (1 mentions) Anti fcγrii fcγriii 2.4g2, by BD (1 mentions) Il 2 apc, by BioLegend (1 mentions) Igm fitc, by BioLegend (1 mentions) Plus brefeldin a, by Thermo Fisher Scientific (1 mentions) Cd138 apc, by BioLegend (1 mentions) Cd5 pe cy7, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD1d-PE(clone PE anti-mouse CD1d PE-conjugated anti mouse α-GalCer:CD1d complex (L363,

2 protocols using cd1d pe

1

Immune Cell Characterization by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure used in this study has been published previously (14 (link),26 (link)). Briefly, the following anti-mouse monoclonal antibodies were purchased from BD Biosciences: Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (1A8; 560602), Ly6C-fluorescein isothiocyanate (FITC) (AL-21; 553104), CD4-allophycocyanin (APC) (RM4-5; 553051), CD25-FITC (7D4; 553071), and CD19-APC (1D3; 550992). The following antibodies were purchased from BioLegend: CD45-FITC (30F-11;103108), CD45-PerCP-Cy5.5 (30F-11;103132), CD45-brilliant violet (BV510) (30F-11;103138), CD11b-APC/Fire750 (M1/70;101262), CD244-Alexa Fluor 647 (2B4;133509), F4/80-phycoerythrin (PE) (BM8;123110), PD-L1-BV421 (10F.9G2;124315), CD8a-APC/Fire 750 (53-6.7;100766), CD8a-PE (53-6.7; 100708), CD127-BV510 (A7R34;135033), CD1d-PE (1B1;123509), and CD5-BV421 (53-7.3;100618). The isolated immune cells were stained in a 96-round-bottom-well plate for 20 min at 4°C and washed with PBS containing 1% bovine serum albumin. Sorting of Ly6G+CD244+ and Ly6G+CD244− cells was conducted on a FACSAria instrument (BD Biosciences). Flow cytometric data were obtained by a FACS Verse instrument (BD Biosciences) and analyzed by FlowJo software (TreeStar, Inc.). CD45-gated cells were analyzed in this study.
Sugita Y., Yamashita K., Fujita M., Saito M., Yamada K., Agawa K., Watanabe A., Fukuoka E., Hasegawa H., Kanaji S., Oshikiri T., Matsuda T., Nakamura T., Suzuki S, & Kakeji Y. (2021). CD244+ polymorphonuclear myeloid-derived suppressor cells reflect the status of peritoneal dissemination in a colon cancer mouse model. Oncology Reports, 45(6), 106.
+ Open protocol
+ Expand
2

B Cell Immunophenotyping and Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
B cells were washed once in cold PBS containing 0.1% BSA (FACS buffer) before blocking with anti-FcγRII/FcγRIII (2.4G2, BD Pharmingen, San Diego, CA). Stainings were performed on ice using conjugated mAbs (eBioscience, San Diego, CA, if not stated otherwise), diluted 1:300 in FACS buffer for surface marker and 1:200 for intracellular cytokine staining followed by incubation for 20 min. After washing with FACS buffer, cells were analysed on a FACS Canto II (BD) using FlowJo software (Tree star, Ashland, OR). The following Abs were used: B220-FITC (#11-0452-86), CD5-PE-Cy7 (#25-0051-81), CD1d-PE (#12-0011-81), CD138-APC (#142506, Biolegend), IgM-FITC (#11-5890-85), IgG1-PE (#12-4015-82), CD4-FITC (#11-0041-82), IL-2-APC (#17-7021-81), IFNγ-APC (#17-7311-82) and IL-10-PerCP (#45-7101-80). Abs against TNF−α−PE (#130-092-245) and IL-17-PE (#130-094-296) were from Miltenyi Biotec. For intracellular staining, the fixation and permeabilization kit (Plus Brefeldin A; eBioscience, Cat. no. 88-8823-88) was used according to manufacturer's recommendation.
Alrefai H., Muhammad K., Rudolf R., Pham D.A., Klein-Hessling S., Patra A.K., Avots A., Bukur V., Sahin U., Tenzer S., Goebeler M., Kerstan A, & Serfling E. (2016). NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. Nature Communications, 7, 11724.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!