Compound microscope dm 2500

Agar plugs (6 mm diam.) were taken from the edge of actively growing cultures on PDA and transferred on to the centre of 9 cm diam Petri dishes containing 2% tap water agar supplemented with sterile pine needles (PNA; Smith et al. 1996 (link)) and potato dextrose agar (PDA) and incubated at 20–21 °C under a 12 h near-ultraviolet light/12 h dark cycle to induce sporulation as described in recent studies (Gomes et al. 2013 (link), Lombard et al. 2014 (link)). Colony characters and pigment production on PNA and PDA were noted after 10 d. Colony colours were rated according to Rayner (1970) . Cultures were examined periodically for the development of ascomata and conidiomata. The morphological characteristics were examined by mounting fungal structures in clear lactic acid and 30 measurements at 1000× magnification were determined for each isolate using a Leica compound microscope (DM 2500) with interference contrast (DIC) optics. Descriptions, nomenclature and illustrations of taxonomic novelties are deposited in MycoBank (www.MycoBank.org; Crous et al. 2004b ).

Colony characteristics of cultures on potato dextrose agar (PDA) medium were recorded after 7 d incubation at 25 °C. Fungal morphology was recorded from colonies grown in the dark for 14 d at 25 °C on PDA. The morphological characteristics were examined by mounting fungal structures in clear lactic acid and 30 measurements at ×1000 magnification were determined for each isolate using a Leica compound microscope (DM 2500) with interference contrast (DIC) optics. Descriptions, nomenclature, and illustrations of taxonomic novelties are deposited in MycoBank [22 ].

Agar plugs (6 mm diam.) were taken from the edge of actively-growing cultures on PDA and transferred on to the centre of 9 cm diam. Petri dishes containing 2% tap water agar, supplemented with sterile pine needles (PNA; Smith et al. 1996 (link)) and potato dextrose agar (PDA) and incubated at 25 °C under a 12 h near-ultraviolet light/12 h dark cycle to induce sporulation, as described in recent studies (Gomes et al. 2013 (link); Lombard et al. 2014 (link)). Colony characters and pigment production on PNA and PDA were noted in the 10-day culture. Colony features were rated according to the colour charts of Rayner (1970) . Cultures were examined periodically for the development of conidiomata. The microscopic examination was based on the morphological features of conidiomata obtained from the fungal growth, mounted in clear lactic acid. At least 30 conidia were measured to calculate the mean size/length. Micro-morphological observations were done at 1000× magnification using a Leica compound microscope (DM 2500) with interference contrast (DIC) optics. Descriptions, nomenclature and illustrations of taxonomic novelties were deposited at MycoBank (www.MycoBank.org).

Agar plugs (6 mm diam.) were taken from the edge of actively growing cultures on PDA and transferred on to the centre of 9 cm diam. Petri dishes containing 2% tap water agar supplemented with sterile pine needles (PNA; Smith et al. (1996) (link)) and potato dextrose agar (PDA) and incubated at 25 °C under a 12 h near-ultraviolet light/12 h dark cycle to induce sporulation as described in recent studies (Gomes et al. 2013 (link); Lombard et al. 2014 (link)). Colony characters and pigment production on PNA and PDA were noted after 10 d. Colony colours were rated according to Rayner (1970) . Cultures were examined periodically for the development of ascomata and conidiomata. The morphological characteristics were examined by mounting fungal structures in clear lactic acid and 30 measurements at 1000× magnification were determined for each isolate using a Leica compound microscope (DM 2500) with interference contrast (DIC) optics. Descriptions, nomenclature and illustrations of taxonomic novelties are deposited in MycoBank (Crous et al. 2004a ).

Agar plugs (6 mm diam) were taken from the edge of actively growing cultures on PDA and transferred onto the centre of 9 cm diam Petri dishes containing 2% tap water agar supplemented with sterile pine needles (PNA) (Smith et al. 1996 (link)) and potato dextrose agar (PDA) and incubated at 25 °C under a 12 h near-ultraviolet light/12 h dark cycle to induce sporulation as described in recent studies (Gomes et al. 2013 (link); Lombard et al. 2014 (link)). Colony characters and pigment production on PNA and PDA were noted in the 10-day culture. Colony features were rated according to the color charts of Rayner (1970) . Cultures were examined periodically for the development of conidiomata. The microscopic examination was based on the morphological features of conidiomata obtained from the fungal growth mounted in clear lactic acid. At least 30 conidiomata and conidia were measured to calculate the mean size/length. Micro-morphological observations were done at ×1000 magnification using a Leica compound microscope (DM 2500) with interference contrast (DIC) optics. Descriptions, nomenclature, and illustrations of taxonomic novelties were deposited at MycoBank (www.MycoBank.org) (Crous et al. 2004 ).