Proton NMR spectra of all imidazolium-bridged dinucleotides were acquired on a Varian Oxford AS-400 NMR spectrometer (for details see the Supplementary Information). Proton NMR peak assignments were performed with the help of coupling constants and 2D COSY NMR. For all the hetero-dinucleotide intermediates, the two different nucleotides in the molecule have well separated H1′ hydrogen peaks. The 3JH1′-H2′ coupling constant was measured from the NMR peak corresponding to H1′ only, because the H2′ peak overlaps with other peaks for most of the bridged dinucleotides. The ratio of 3′-endo to 2′-endo sugar pucker conformation was calculated based on the equation: C3′-endo/2′-endo = J/(10 – J) where J = 3JH1′-H2′ (11 (link)).
Oxford as 400 nmr spectrometer
Manufactured by Agilent Technologies
The Oxford AS-400 NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical and research applications. It operates at a magnetic field strength of 9.4 Tesla and is capable of conducting 1H, 13C, and other common NMR experiments. The spectrometer features a superconducting magnet and advanced electronics to provide reliable and consistent data acquisition.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using oxford as 400 nmr spectrometer
1
Proton NMR Analysis of Imidazolium-Bridged Dinucleotides
2
Conformational Analysis of Imidazolium-Bridged Dinucleotides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proton NMR spectra of all imidazolium-bridged dinucleotides were acquired on a Varian Oxford AS-400 NMR spectrometer (for details see the Supplementary Information). Proton NMR peak assignments were performed with the help of coupling constants and 2D COSY NMR. For all the hetero-dinucleotide intermediates, the two different nucleotides in the molecule have well separated H1ʹ hydrogen peaks.
The 3 JH1ʹ-H2ʹ coupling constant was measured from the NMR peak corresponding to H1' only, because the H2' peak overlaps with other peaks for most of the bridged dinucleotides. The ratio of the fraction of 3'endo to 2'-endo sugar pucker conformation was calculated based on the equation: C3'-endo/2'-endo = J/(10-J) where J = 3 JH1ʹ-H2ʹ.( 11)
The 3 JH1ʹ-H2ʹ coupling constant was measured from the NMR peak corresponding to H1' only, because the H2' peak overlaps with other peaks for most of the bridged dinucleotides. The ratio of the fraction of 3'endo to 2'-endo sugar pucker conformation was calculated based on the equation: C3'-endo/2'-endo = J/(10-J) where J = 3 JH1ʹ-H2ʹ.( 11)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!