Extracellular content of trans-resveratrol and trans-piceid was determined as described by Pietrowska-Borek et al. [3 (link),6 (link)]. For this, 20 µL of diluted and filtered (Anopore 0.2 µm) samples were analyzed by HPLC in the UV–VIS range using a LiChrospher 100 RP-18 column (250 × 4 mm, 5 µm; Merck, Darmstadt, Germany). Gradient elutions were performed with 0.05% TFA (solvent A) and 0.05% TFA in methanol:acetonitrile (60:40 v/v; solvent B): 0 min, 10% B; 5 min, 15% B; 40 min, 35% B; 45 min, 65% B; 50 min, 65% B; and 55 min, 10% B, setting the flow rate at 1 mL min−1. To determine the intracellular content of trans-resveratrol and trans-piceid, 200 mg of freeze-dried cells were extracted overnight with 4 mL of methanol at 4 °C with continuous shaking, and then 20 µL of each sample was analyzed on a LiChrospher 100 RP-18 column as described above. trans-Resveratrol and trans-piceid were identified (at 308 nm) and quantified by comparison with standards of commercial trans-resveratrol (Sigma-Aldrich, St. Louis, MO, USA) and trans-piceid (ChromaDex, Los Angeles, CA, USA) using respective calibration curves.
Trans resveratrol
Manufactured by Merck Group
Sourced in United States, Germany, France, Spain, Italy
Trans-resveratrol is a naturally occurring polyphenolic compound found in various plants, including grapes, berries, and peanuts. It is a key ingredient in many dietary supplements and research applications. Trans-resveratrol has been studied for its potential antioxidant and anti-inflammatory properties.
Automatically generated - may contain errors
Lab products found in correlation
Catechin, by Merck Group (21 mentions)
Gallic acid, by Merck Group (21 mentions)
Epicatechin, by Merck Group (19 mentions)
Quercetin, by Merck Group (14 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (13 mentions)
P coumaric acid, by Merck Group (11 mentions)
Myricetin, by Merck Group (10 mentions)
Caffeic acid, by Merck Group (10 mentions)
Trans piceid, by Merck Group (10 mentions)
Ferulic acid, by Merck Group (10 mentions)
Syringic acid, by Merck Group (8 mentions)
Acetonitrile, by Merck Group (8 mentions)
Vanillic acid, by Merck Group (7 mentions)
Kaempferol, by Merck Group (7 mentions)
Rutin, by Merck Group (7 mentions)
Protocatechuic acid, by Merck Group (7 mentions)
Milli q system, by Merck Group (7 mentions)
Hydrochloric acid (hcl), by Merck Group (7 mentions)
Folin ciocalteu reagent, by Merck Group (7 mentions)
Sodium carbonate, by Merck Group (7 mentions)
107 protocols using trans resveratrol
1
Quantification of trans-Resveratrol and trans-Piceid
2
Quantitative HPLC Analysis of Resveratrol in Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples for HPLC analysis were prepared from frozen tumor specimens and pooled ovarian tissues by a method described elsewhere.13 (link) Briefly, a frozen tissue sample was sectioned into 7 mm slices using a freezing microtome, collected in a centrifuge tube, and extracted with 416 μl methanol and 84 μl internal standard [IS, 1, 8-dihydroxyanthraquinone, 200 μg/ml; (Sigma-Aldrich)]. The tissue homogenates were vortexed for 5 min and centrifuged at 12,000 rpm for 10 min at 4 °C. The supernatant was then transferred to a clean tube. The residue was extracted twice with 1 ml methanol by 5 min of vigorous agitation, followed by centrifugation. The combined organic solvent of the supernatants was evaporated to a final volume of 400 μl and subsequently placed in a sealed amber vial for HPLC analysis.23 (link) To improve the sensitivity and precision of quantification, 1, 8-dihydroxyanthraquinone was used as the internal standard and trans-resveratrol (Sigma-Aldrich) as the standard to construct a standard curve using previously described methods.13 (link)
3
NMR Analysis of Epinephrine and Resveratrol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials. Epinephrine (EP) and trans-resveratrol (3,5,4'-trihydroxy-trans-stilbene, RSV) were purchased from Sigma Aldrich Chemie GmbH (Buchs, Switzerland) and used without further [17] . The FID (Free Induction Decay) was processed using an exponential window function with line broadening of 1 Hz. The selective and non-selective spin-lattice relaxation rates refer to the H-6 of epinephrine. Since in general the recovery of proton longitudinal magnetization after a 180° pulse is not a single exponential, due to the sum of different relaxation terms, the selective spin-lattice relaxation rates were calculated using the initial slope approximation and subsequent three parameter exponential regression analysis of the longitudinal recovery curves. The maximum experimental error in the relaxation rate measurements was 5%. The affinity index was calculated by linear regression analysis of the experimental data.
All the spectra were processed using the Bruker Software XWINNMR, version 2.5 on Silicon
Graphics O 2 equipped with RISC R5000 processor, working under the IRIX 6.3 operating system.
All the spectra were processed using the Bruker Software XWINNMR, version 2.5 on Silicon
Graphics O 2 equipped with RISC R5000 processor, working under the IRIX 6.3 operating system.
4
Phenolic Compounds: Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Luteolin (≥98%; CAS number 491-70-3); apigenin (≥95%; CAS number 520-36-5); trans resveratrol (CAS number 501-36-0); oleuropein (≥98%; CAS number 36619-42-4); verbascoside (≥99%; CAS number 61276-17-3); isoverbascoside (≥95%; CAS number 61303-13-7); tyrosol (≥95%; CAS number 501-94-0); vanillic acid (≥97%; CAS number 121-34-6); cinnamic acid (≥99%; CAS number 140-10-3); ferulic acid (≥99%; CAS number 537-98-4); p-coumaric acid (≥98%; CAS number 501-98-4); 4-hydroxybenzoic acid (≥99%; CAS number 99-96-7); 3-hydroxybenzoic acid (≥99%; CAS number 99-06-9) were purchased from Sigma Aldrich (St. Louis, MO, USA); secologanoside (>95%) were bought from ChemFaces Biochemical Co., Ltd. (Hubei, China); hydroxytyrosol (>95%) were obtained by Indofine (Hillsborough, NJ, USA); oleuropein-aglycone monoaldehyde (>95%) were purchased from Extrasynthese (Genay, France). Ligstroside were purchased from Wuhan Golden Wing Industry & Trade Co., Ltd. (Wuhan, China), the ligstroside-aglycone monoaldehyde (>95%) was isolated by chromatographic method and identity by nuclear magnetic resonance (NMR). The other chemicals were the analytical (Sigma Aldrich, St. Louis, MO, USA).
5
Trans-resveratrol and PRMT5 Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trans-resveratrol was purchased from Sigma-Aldrich (Cat#R5010, St. Louis, MO, USA).
GSK591, a specific inhibitor of PRMT5, was purchased from Sigma-Aldrich (Cat# SML1751, St
Louis, MO, USA). The study was approved by the Ethical Committee of Ruijin Hospital
Affiliated to Shanghai Jiao Tong University School of Medicine (LWEC201603).
GSK591, a specific inhibitor of PRMT5, was purchased from Sigma-Aldrich (Cat# SML1751, St
Louis, MO, USA). The study was approved by the Ethical Committee of Ruijin Hospital
Affiliated to Shanghai Jiao Tong University School of Medicine (LWEC201603).
6
Biodegradable Resveratrol-Loaded Particles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particles were produced by an oil-in-water emulsion-solvent evaporation technique. The oil phase consisted of a 3:1 mixture of dichloromethane (Sigma, St. Louis, MO) and ethanol (Sigma, St. Louis, MO) containing 6% w/v PLG (purchased from Evonik, Birmingham, AL, 75:25 mol ratio lactide to glycolide, 0.76 dL/g) and 10 mg/mL of trans-resveratrol (R5010, Sigma, St. Louis, MO). The aqueous phase consisted of 1% w/v poly(vinyl alcohol) (Sigma, St. Louis, MO) dissolved in ultrapure water. The oil phase was homogenized with the aqueous phase using a 1:7 volume ratio with a benchtop homogenizer (Kinematica, Bohemia, NY). The emulsion was then immediately added to ultrapure water and stirred for 5 h to allow particles to harden and organic solvents to evaporate. Particles were collected via centrifugation, washed with ultrapure water, and lyophilized.
7
Graphene Oxide Synthesis and Resveratrol Electrochemical Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Graphene oxide was obtained from graphite nanopowders (Sinopharm Chemical Reagent Co., China) by a modified Hummers method [35 (link)]. trans-Resveratrol was purchased from Sigma-Aldrich. 0.04 M Britton-Robinson (BR) buffer solution was prepared from an acidic solution which contained 0.04 M H3PO4, HOAc, and H3BO3 by adjusting to different pH using 0.2 M NaOH. All other reagents were supplied by commercial suppliers and used without a filtration system. The water used was purified by a Millipore M-Q purification system (>18 MΩ).
8
Bioactive Compound Standards for Antioxidant Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Catechin hydrate (>98%), trans-resveratrol (>99%), quercetin 3-O-glucuronide (≥95%), cyanin chloride (≥90%), ferulic acid (99%), kaempferol, p-coumaric acid, kaempferol 3-O-rutinoside (98%), myricetin, isorhamnetin and rutin (quercetin 3-O-rutinoside) hydrate (>94%) were obtained from Sigma-Aldrich (Darmstadt, Germany). Sodium carbonate anhydrous (99%), Folin–Ciocalteu reagent, and glycerol anhydrous (99%) were purchased from Penta (Praha, Czechia). Formic acid (99%), L-ascorbic acid and citric acid (≥99.5%) were from Carlo Erba (Milan, Italy). All solvents used for chromatography were of appropriate (HPLC) grade. The radical probe 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin hydrate (97%) and caffeic acid (99%) were from Alfa Aesar (Karlsruhe, Germany). Iron chloride hexahydrate (FeCl3 • 6H2O) was purchased from Merck (Darmstadt, Germany). 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) was from Fluka (Steinheim, Germany).
9
Enzyme Inhibition and Antioxidant Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tyramine hydrochloride, benzylamine hydrochloride, amine oxidase inhibitors, quercetin, trans-resveratrol, and other reagents were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France), except otherwise specified.
10
Chagas Disease Treatment Approaches
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were infected intraperitoneally (i.p.) with 102 blood trypomastigote forms of the type I Colombian strain of T. cruzi. Treatments were performed daily for 30 days from the establishment of CCC (60 dpi) by i.p. injection of 15 mg.kg-1 trans-resveratrol (Sigma, 10% ethanol/PBS), vehicle (10% ethanol/PBS), 5 mg.Kg-1 EX527 (0.1% DMSO, Sigma), or peroral administration of 40 mg.Kg-1 resveratrol (10%ethanol-PBS), 500 mg.kg-1 metformin (Merck, dissolved in water), 100 mg.kg-1 tempol (Sigma, dissolved in water), benznidazole (Rochagan, 25 mg/Kg, dissolved in water) and vehicle (water or 10%ethanol-PBS).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!