V plex proinflammatory panel 1 human kit

Manufactured by Mesoscale

Sourced in United States

The V-PLEX Proinflammatory Panel 1 Human Kit is a multiplex assay designed for the quantitative measurement of multiple proinflammatory analytes in human biological samples. The kit utilizes Mesoscale's proprietary electrochemiluminescence technology to enable the detection and quantification of these analytes in a single sample.

Automatically generated - may contain errors

Lab products found in correlation

Meso quickplex sq 120, by Mesoscale (3 mentions) V plex human mcp 1 kit, by Mesoscale (3 mentions) Quickplex sq 120, by Mesoscale (3 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) V plex chemokine panel 1 human kit, by Mesoscale (2 mentions) Tissuelyser, by Qiagen (1 mentions) Pierce lal chromogenic endotoxin quantitation kit, by Thermo Fisher Scientific (1 mentions) Synergy 2 multi mode microplate reader, by Agilent Technologies (1 mentions) Quantikine elisa assay kit, by R&D Systems (1 mentions) Msd quickplex sq120, by Mesoscale (1 mentions) Discovery workbench analysis software, by MSD (1 mentions) V plex proinflammatory panel 1 kit, by Mesoscale (1 mentions) Discovery workbench 4, by MSD (1 mentions) Meso quickplex sq 120, by MSD (1 mentions) Sector imager 6000, by Mesoscale (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Brefeldin a, by Merck Group (1 mentions) Lidocaine, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Mesoscale (1 mentions)

Spelling variants of this product

V-PLEX Proinflammatory Panel 1 (28 citations)

57 protocols using v plex proinflammatory panel 1 human kit

Inflammatory Marker Analysis in Cohorts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inflammatory markers (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and TNF-α) were analyzed in blood samples, stored in − 80 °C until analysis, from both patient cohorts and controls by using a V-PLEX Proinflammatory Panel 1 (human) Kit (MesoScaleDiscovery, Rockville, MD, USA), following the manufacturer’s protocol. Samples were run in duplicates and mean values were used. Samples from both patient cohorts and controls were run proportionally in each batch. For samples with a mean value below the lower detection limit, the value was set to 50% of the lower detection limit. For 4 of 10 inflammatory markers (IL-6, IL-8, IL-10, and TNF-α), <=5% of the samples were below the lower detection limit, whereas for the rest of the markers, > = 25% of samples were below lower detection limit (Fig. S1). Thus, we focused further analysis on IL-6, IL-8, IL-10, and TNF-α. Inflammatory markers were divided into two groups; ‘High’ – above the median of the controls, and ‘Low’ – below the median of the controls.

Ekedahl H., Isaksson S., Ståhl O., Bogefors K., Romerius P., Eberhard J, & Giwercman A. (2022). Low-grade inflammation in survivors of childhood cancer and testicular cancer and its association with hypogonadism and metabolic risk factors. BMC Cancer, 22, 157.

+ Open protocol

+ Expand

Measuring Quality of Life, Sleep, and Inflammation in ICs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quality of life in ICs was measured with the World Health Organization (WHO)-5 questionnaire (40 ) (α = .90). Higher scores indicate better quality of life. Sleep quality was measured with the Pittsburgh Sleep Quality Index (41 (link)). Higher scores indicate poorer sleep quality. Emotional closeness was assessed with the discrepancy between two items, rated on a scale from 0 to 10, namely, actual and ideal emotional closeness (42 ). Negative scores indicate less emotional closeness than wanted.
Pro-inflammatory markers included high sensitivity-CRP, tumor necrosis factor-alpha (TNFα), interleukin (IL)-1, and IL-6. To control for diurnal variation, samples were obtained within ±3 hours of each other. Serum was analyzed with the high-sensitivity CRP enzyme-linked immunosorbent assay kit and the V-PLEX Proinflammatory Panel 1 (Human) kit, Meso Scale Discovery, Rockville, MD. Higher scores indicate higher levels of inflammation.

O’Toole M.S., Mennin D.S., Applebaum A., Weber B., Rose H., Fresco D.M, & Zachariae R. (2019). A Randomized Controlled Trial of Emotion Regulation Therapy for Psychologically Distressed Caregivers of Cancer Patients. JNCI Cancer Spectrum, 4(1), pkz074.

+ Open protocol

+ Expand

Inflammatory Biomarkers in Stool and Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inflammatory biomarkers were measured in stool and plasma samples. Plasma supernatants were aliquoted and batch analyzed. The rectal swabs that were stored at room temperature were placed in 1mL phosphate-buffered saline (PBS [pH 7.4]), and the stool was extracted using Qiagen TissueLyser at 4°C. After centrifugation at 260 ×g for 10min to clear debris, supernatants were aliquoted and batch analyzed. Plasma and stool samples were transported to the Emory Multiplexed Immunoassay Core for processing. Lipopolysaccharide (LPS) was measured in plasma using an enzyme-linked immunosorbent assay (ELISA) kit (Pierce LAL Chromogenic Endotoxin Quantitation Kit, Thermo-Fisher Scientific, Inc., Waltham, MA). Cytokines (interleukin [IL]-1β, IL-2, IL-4 IL-6, IL-8, IL-10, IL-12p70, IL-13, tumor necrosis factor [TNF], and interferon [IFN]-γ) were measured in stool and plasma samples using a V-PLEX Pro-inflammatory Panel 1 Human Kit in a 96-well plate on a QuickPlex instrument (Meso Scale Diagnostics, Rockville, MD). LPS-binding protein (LBP) and high-sensitivity C-reactive protein (hsCRP) were measured in stool and plasma samples using the Meso Scale Human LBP and CRP plates, respectively. All samples were processed in duplicate.

HERTZBERG V.S., SINGH H., FOURNIER C.N., MOUSTAFA A., POLAK M., KUELBS C.A., TORRALBA M.G., TANSEY M.G., NELSON K.E, & GLASS J.D. (2021). Gut microbiome differences between amyotrophic lateral sclerosis patients and spouse controls. Amyotrophic lateral sclerosis & frontotemporal degeneration, 23(1-2), 91-99.

+ Open protocol

+ Expand

Cytokine Profiling of Activated T-Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

T-Peripheral blood mononuclear cells and T-pure cells were unstimulated or TCR-stimulated for 72 h as described above. Subsequently, cell culture supernatant was harvested and analyzed for the presence of IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, TNFα, and IFNγ, using the V-plex pro-inflammatory panel 1 human kit (Meso scale discovery) according to manufactures protocol. Cytokines were measured in the unit pg/ml. IL-2 was excluded from the analysis, as rhIL-2 was supplemented to the culture media.

Pedersen J.G., Egedal J.H., Packard T.A., Thavachelvam K., Xie G., van der Sluis R.M., Greene W.C., Roan N.R, & Jakobsen M.R. (2021). Cell-Extrinsic Priming Increases Permissiveness of CD4+ T Cells to Human Immunodeficiency Virus Infection by Increasing C–C Chemokine Receptor Type 5 Co-receptor Expression and Cellular Activation Status. Frontiers in Microbiology, 12, 763030.

+ Open protocol

+ Expand

Cytokine secretion in alveolar model

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ability of the model to increase the secretion of pro-inflammatory cytokines after exposure to lipopolysaccharide (LPS) was evaluated. The alveolar model was exposed directly at the ALI to 0.625 and 6.25 μg/cm² LPS and the level of IL-8, a known pro-inflammatory cytokine, was measured from the cellular undernatants at 24 h post-exposure. The measurement was done using a Quantikine ELISA assay kit (R&D systems, Minneapolis MN, USA), according to the manufacturer’s instructions. The absorbance was measured using a Synergy 2 Multi-Mode Microplate Reader (BioTek).
At 6 h and 24 h post-exposure to AgNWs, aliquots of media were taken from the basolateral compartment and stored at − 80 °C until analysis. The concentrations of IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IFN-γ and TNF-α were measured using a V-PLEX pro-inflammatory Panel 1 Human kit according to the manufacturer’s instructions (Meso Scale Diagnostics, Rockville, US). Quantification of the inflammatory cytokines was performed on a MSD QuickPlex SQ 120 (Meso Scale Diagnostics) following the manufacturer’s protocols.

Fizeșan I., Cambier S., Moschini E., Chary A., Nelissen I., Ziebel J., Audinot J.N., Wirtz T., Kruszewski M., Pop A., Kiss B., Serchi T., Loghin F, & Gutleb A.C. (2019). In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires. Particle and Fibre Toxicology, 16, 14.

+ Open protocol

+ Expand

Cytokine Profiling in Stroke Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

In separate experiments, concentration of 10 different cytokines including interferon (IFN)-γ, IL-10, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8 and tumor necrosis factor (TNF)-α was determined with a V-PLEX pro-inflammatory panel 1 (human) kit (Meso Scale Discovery, Gaithersburg, MD) according to the manufacturer’s instructions. Briefly, total leukocytes were isolated and plated on 96 well dishes as described above. Cells were cultured with 10% control and stroke patients’ serum and incubated in a saturated humidity atmosphere at 37°C containing 95% ambient air and 5% CO₂ for 1, 3 and 6 hours. Control and stroke patients’ serum without total leukocytes were used as negative control groups. Following the incubation, the medium was collected and centrifuged at 300 g for 5 minutes at 4°C, after which the supernatant was transferred to a new tube. Subsequently, the tube was centrifuged at 20,000 g for 5 minutes at 4°C. The cell-free conditioning media was stored at −80°C until the analysis.

Asano S., O’Connell G.C., Lemaster K.C., DeVallance E.R., Branyan K.W., Simpkins J.W., Frisbee J.C., Barr T.L, & Chantler P.D. (2017). Circulating Leukocytes Perpetuate Stroke-Induced Aortic Dysfunction. Experimental physiology, 102(10), 1321-1331.

+ Open protocol

+ Expand

Multiplex Cytokine Profiling of MTB Antigens

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatants of QGIT samples from nil (control) and MTB antigen-stimulated tubes were stored at −80° C. Cytokines in the supernatants were measured using a multiplex cytokine panel (V plex pro-inflammatory panel 1 human kit) from Meso Scale Discovery (MSD) (Rockville, MD, USA). Plates were read on the MSD detector (MESO Quickplex SQ120). Calibration curve and analyte concentration calculations were performed using MSD DISCOVERY WORKBENCH® analysis software.

Epstein R.L., Bhagavathula M., Saag L.A., Verma S., Kan C.K., Mesick J., Kamineni P., White L.F., Barnett E., Salgame P, & Hochberg N.S. (2019). QuantiFERON-TB Gold In-Tube Reliability for Immigrants with Parasitic Infections, Boston. The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 23(4), 482-490.

+ Open protocol

+ Expand

Astroglia Cytokine Response to WIN and IL-1β

Check if the same lab product or an alternative is used in the 5 most similar protocols

Astroglia cultures were treated with vehicle, WIN, IL-1β, or WIN+IL-1β in 6 well plates for 24 h. Supernatant was collected and analyzed for inflammatory cytokines, TNF-α, IL-6, IL-12p70, interferon (IFN) γ, IL-4, and IL-10 using V-PLEX Proinflammatory Panel 1 Human Kit (Mesoscale, cat. no. K15049D-4) as per manufacturer’s instructions. Samples were tested in duplicate.

Swinton M.K., Carson A., Telese F., Sanchez A.B., Soontornniyomkij B., Rad L., Batki I., Quintanilla B., Perez-Santiago J., Achim C.L., Letendre S., Ellis R.J., Grant I., Murphy A.N, & Fields J.A. (2019). Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: implications for therapeutic targeting of astroglia. Neurobiology of disease, 130, 104502.

+ Open protocol

+ Expand

Cytokine Profiling in Human Serum and Mouse Lungs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inflammatory and immune response cytokines (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and TNF-α) were measured in human serum samples in singlicate using the V-PLEX Proinflammatory Panel 1 Human Kit (Meso Scale Discovery, Gaithersburg, MD) according to manufacturer protocol. From mouse lung supernatants, the concentrations of cytokines (CXCL1, IFN-γ, IL-6, IL-10, and TNF-α) were measured in singlicate using the V-PLEX Proinflammatory Panel 1 Kit (Meso Scale Discovery) according to manufacturer protocol. Overall, there was low detection of cytokines prior to infection, therefore we analyzed induction.

Potluri T., Fink A.L., Sylvia K.E., Dhakal S., Vermillion M.S., vom Steeg L., Deshpande S., Narasimhan H, & Klein S.L. (2019). Age-associated changes in the impact of sex steroids on influenza vaccine responses in males and females. NPJ Vaccines, 4, 29.

+ Open protocol

+ Expand

Comprehensive Cytokine Profiling Using Electrochemiluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of IL-1β, IL-2, IL-4, IL-6, IL-8, IL10, IL-12p70, IL-13, TNF-α and IFNγ were analysed via an electrochemiluminescent immunoassay (V-PLEX Proinflammatory Panel 1 Human Kit) according to the manufacturer’s instructions (Meso Scale Discovery (MSD), Gaithersburg, MD, USA) using the Meso Quick Plex SQ 120 (Figs 1, 2 and 4–6). Raw data were analysed using the Discovery Workbench 4.0 software (MSD). For one experiment IL-8 levels were determined by a commercially available enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Inc., Minneapolis, MN, USA) (Fig. 3).

Bleidorn J., Alamzad-Krabbe H., Gerhards B., Kraus T., Brand P., Krabbe J, & Martin C. (2019). The pro-inflammatory stimulus of zinc- and copper-containing welding fumes in whole blood assay via protein tyrosine phosphatase 1B inhibition. Scientific Reports, 9, 1315.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!