Log In Sign up
The largest database of trusted experimental protocols

C57bl 6j male and female mice

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in United States, Montenegro

C57BL/6J male and female mice are laboratory animals commonly used in biomedical research. They are inbred strains of the house mouse (Mus musculus) that have been widely characterized and utilized as model organisms.

Automatically generated - may contain errors

Lab products found in correlation

5p76 prolab isopro rmh 3000, by LabDiet (3 mentions) Cd 1 recipient females and vasectomized males, by Charles River Laboratories (3 mentions) Rnalater, by Thermo Fisher Scientific (2 mentions) Ain 76a, by Merck Group (1 mentions) Stigmasterol, by Merck Group (1 mentions) T0901317, by Cayman Chemical (1 mentions) L4129, by Merck Group (1 mentions) Sprague dawley rat, by Charles River Laboratories (1 mentions) Streptozotocin (stz), by MP Biomedicals (1 mentions) Ionomycin, by Bio-Techne (1 mentions) Fitc pna, by Merck Group (1 mentions) Pbs buffer, by Corning (1 mentions) 3 isobutyl 1 methylxanthine, by Merck Group (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Avantor (1 mentions) Polyethylene glycol 400, by Merck Group (1 mentions) Esr1 cre mice, by Jackson ImmunoResearch (1 mentions) Tph2 icreer, by Jackson ImmunoResearch (1 mentions) Rosa26 lsl tdtomato, by Jackson ImmunoResearch (1 mentions)

43 protocols using c57bl 6j male and female mice

1

Dietary Modulation of Lipid Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 J male and female mice were purchased from Jackson Laboratory (Bar Harbor, ME) at 6 weeks of age and housed in individually ventilated cages (14:10, light: dark) with free access to feed and water. Test diets were formulated from control diet (Research Diets Inc., AIN-76A) and supplemented with either 0.3% (w/w) stigmasterol (Sigma-Aldrich, Product Number S2424) or 0.015% (w/w) T0901317 (Cayman Chemical, Product Code 71810). At 8 weeks of age, mice were singly housed with access to water and control diet for 3 days. On day four, mice were randomized to receive either control diet, stigmasterol-, or T0901317-supplemented diet (ad libidum) for an additional 4 days. On Day 8, mice were euthanized for plasma and tissue dissection (n=8 per sex). Mice were euthanized by carbon dioxide and exsanguination via cardiac puncture at termination. Liver and small intestine sections were dissected, snap frozen in liquid nitrogen, and stored in −80 °C. Small intestine was divided into three equal sections representing the duodenum, jejunum, and ileum. Blood was collected in EDTA-coated tubes, centrifuged at 2000×g for 15 min at 4 °C, and plasma stored at −80 °C.
Lifsey H.C., Kaur R., Thompson B.H., Bennett L., Temel R.E, & Graf G.A. (2019). Stigmasterol stimulates transintestinal cholesterol excretion independent of liver X receptor activation in the small intestine. The Journal of nutritional biochemistry, 76, 108263.
+ Open protocol
+ Expand
2

Husbandry of C57Bl6/J Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57Bl6/J male and female mice were purchased from The Jackson Laboratory and housed at the University of Washington. All mice were maintained at 21 °C on a 14/10 light/dark cycle at at 30–70% humidity and given standard mouse chow (LabDiet PicoLab® Rodent Diet 20) and water ad libitum unless otherwise specified. This study was reviewed and approved by the University of Washington Institutional Animal Care and Use Committee (IACUC).
Pharaoh G., Ostrom E.L., Stuppard R., Campbell M., Borghardt J.M., Franti M., Filareto A, & Marcinek D.J. (2023). A novel mitochondrial complex I ROS inhibitor partially improves muscle regeneration in adult but not old mice. Redox Biology, 64, 102770.
+ Open protocol
+ Expand
3

Animal Welfare in Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J male and female mice (6–8 weeks) were procured from The Jackson Laboratory (Bar Harbor, ME, USA) and maintained in a pathogen-free environment. All animal studies were pre-approved and carried out in accordance with the University of Texas MD Anderson Cancer Center Institutional Animal Care and Use Committee (IACUC) guidelines. Mice were monitored daily for overall health and euthanized according to IACUC criteria.
O’Hara M.P., Yanamandra A.V, & Sastry K.J. (2024). Immunity from NK Cell Subsets Is Important for Vaccine-Mediated Protection in HPV+ Cancers. Vaccines, 12(2), 206.
+ Open protocol
+ Expand
4

Animal Care and Housing Guidelines

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed all experiments in accordance with the University of North Carolina at Chapel Hill (UNC) Institutional Animal Care
and Use Committee’s guidelines. C57BL/6J male and female mice (aged 7 weeks; Jackson Laboratories) were group or singly housed
within UNC animal facilities on a normal light cycle (lights on at 7 am, lights off at 7 pm) and given food and water ad
libitum.
Luster B.R., Cogan E.S., Schmidt K.T., Pati D., Pina M.M., Dange K, & McElligott Z.A. (2019). Inhibitory transmission in the bed nucleus of the stria terminalis in male and female mice following morphine withdrawal. Addiction biology.
+ Open protocol
+ Expand
5

Lysolecithin Demyelination Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysolecithin (L-α-Lysophosphatidylcholine from egg yolk, Sigma L4129) was diluted to 1% in PBS, and aliquots were frozen at −20°C for single use. Mice at approximately P28 were anesthetized with 1–4% isoflurane and placed in a stereotactic apparatus. The cranium was exposed via midline incision under aseptic conditions, and a hole was drilled in the skull for injection. A 26s-gauge Hamilton syringe was used to inject 1μl lysolecithin into the cingulum using a digital pump at infusion rate of 0.2 μl/min. Stereotactic coordinates used for cingulum were 1.0 mm lateral to midline, 1.0 mm anterior to bregma, −1.5 mm deep to cranial surface. At the completion of infusion, syringe needle remained in place for a minimum of 5 min to minimize backflow of the injection.
C57BL/6J male and female mice (The Jackson Laboratories) were used in the lysolecithin demyelination/remyelination model. Mice were stereotactically injected with 1% lysolecithin at P28 and were injected intraperitoneally with either 50mg/kg small molecule partial agonist LM22A-4 (F. Longo) or vehicle beginning at P35. Mice did not exhibit overt neurological deficits nor sickness behavior following lysolecithin injection.
Geraghty A.C., Gibson E.M., Ghanem R.A., Greene J.J., Ocampo A., Goldstein A.K., Ni L., Yang T., Marton R.M., Paşca S.P., Greenberg M.E., Longo F.M, & Monje M. (2019). Loss of adaptive myelination contributes to methotrexate chemotherapy-related cognitive impairment. Neuron, 103(2), 250-265.e8.
+ Open protocol
+ Expand
6

Microbiome Dynamics in Stressed Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J male and female mice (Jackson Laboratory) were 8 weeks old at the start of the study. All animals were pair housed with paper bedding and maintained on a 12-h light/dark cycle with ad libitum access to food and water. Feces were collected from one mouse per cage to avoid bias associated with coprophagy (the ingestion of feces). For the stress group, we used an n = 12 (6 males and 6 females); for the control group, an n = 12 was used (6 males and 6 females). Fecal samples were collected at three timepoints resulting in 72 microbiome samples total.
Kropp D.R., Rainville J.R., Glover M.E., Tsyglakova M., Samanta R., Hage T.R., Carlson A.E., Clinton S.M, & Hodes G.E. (2024). Chronic variable stress leads to sex specific gut microbiome alterations in mice. Brain, Behavior, & Immunity - Health, 37, 100755.
+ Open protocol
+ Expand
7

Rodent Housing and Handling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments using rats were performed on 300 to 400 g male Sprague-Dawley rats (Charles River Laboratories, Hollister, CA). Rats were housed 3 per cage under a 12-h light/dark cycle in a temperature- and humidity-controlled room at the University of California, San Francisco animal care facility. Food and water were available in home cages, ad libitum. Protocols for rat experiments were approved by the University of California, San Francisco, Institutional Animal Care and Use Committee, and adhered to the National Institutes of Health (NIH) Guidelines for the care and use of laboratory animals.
All studies on mice were performed with C57BL/6J male and female mice (The Jackson Laboratory, Bar Harbor, ME). Experiments in mice were conducted under a protocol approved by the Institutional Animal Care and Use Committee at the University of New England, in accordance with the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the NIH. Mice were housed 3–5 per cage under a 12-h light/dark cycle in a temperature- and humidity-controlled room in the animal care facility at the University of New England, Biddeford campus. Food and water were available in home cages, ad libitum.
Fitzsimons L.A., Staurengo-Ferrari L., Bogen O., Araldi D., Bonet I.J., Jordan E.E., Levine J.D, & Tucker K.L. (2024). The Primary Cilium and its Hedgehog Signaling in Nociceptors Contribute to Inflammatory and Neuropathic Pain. Research Square.
+ Open protocol
+ Expand
8

Chronic Ethanol Exposure in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments were approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh and conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. C57BL/6J male and female mice used for chronic intermittent ethanol vapor (CIEV) exposure, generation of embryos for electroporation, and purchased control groups were procured from The Jackson Laboratory (Bar Harbor, ME). CD-1 recipient females and vasectomized males were procured from Charles River Laboratories, Inc. (Wilmington, MA). Mice were housed in individually ventilated caging under specific pathogen-free conditions with 12-h light/dark cycles (lights on at 7 AM) and had ad libitum access to food (irradiated 5P76 ProLab IsoProRMH3000; LabDiet, St. Louis, MO) and water.
Plasil S.L., Collins V.J., Baratta A.M., Farris S.P, & Homanics G.E. (2022). Hippocampal ceRNA networks from chronic intermittent ethanol vapor-exposed male mice and functional analysis of top-ranked lncRNA genes for ethanol drinking phenotypes. Advances in Drug and Alcohol Research, 2, 10831.
+ Open protocol
+ Expand
9

Murine Husbandry for Metabolic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J male and female mice were purchased from the Jackson Laboratory (Bar Harbor, Maine) at 6 weeks of age and were acclimated to the specific‐pathogen‐free facility for 2 weeks. All mice were singly housed for the duration of the study in standard, ventilated mouse cages within a Thoren Rack Mobile Housing System (Thoren Caging Systems, Inc., Hazleton, Pennsylvania) as described (14, 15). Animal rooms were maintained at 20°C to 22°C on a 12‐hour light‐dark cycle from 6:00 am to 6:00 pm. Animal health was checked daily, and moribund animals were euthanized according to the study protocol. All study protocols were approved by the University of Alabama at Birmingham Institutional Animal Care and Use Committee. Some data from this study have been described elsewhere (15).
Smith DL J.r., Yang Y., Nagy T.R., Patki A., Vasselli J.R., Zhang Y., Dickinson S.L, & Allison D.B. (2018). Weight Cycling Increases Longevity Compared with Sustained Obesity in Mice. Obesity (Silver Spring, Md.), 26(11), 1733-1739.
+ Open protocol
+ Expand
10

Wild-type Mouse Model Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
One‐ to 2.6‐month‐old C57BL/6J male and female mice (wild‐type; WT) from Jackson laboratory (ME, USA) were used for mouse experiments. Animals were maintained at the animal facility at the University of Iowa under controlled environmental conditions, with ad libitum access to food and water. All experiments were carried out per protocols approved by the Institutional Animal Care and Use Committee of the University of Iowa.
Sturgeon M.L., Langton R., Sharma S., Cornell R.A., Glykys J, & Bassuk A.G. (2021). The opioid antagonist naltrexone decreases seizure‐like activity in genetic and chemically induced epilepsy models. Epilepsia Open, 6(3), 528-538.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!