Troglitazone

Manufactured by Fujifilm

Sourced in Japan

Troglitazone is a laboratory equipment product manufactured by Fujifilm. It is a medical compound used in the research and development of pharmaceuticals. The core function of Troglitazone is to serve as a reference standard for analytical testing and quality control procedures.

Automatically generated - may contain errors

Lab products found in correlation

9 protocols using troglitazone

Hepatocyte Organoid Toxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

PHH-derived organoids were seeded on a 10 mg/mL HYDROX-coated 96-well plate. After 14 days of the HYDROX culture, Org-HYDROX were cultured with organoid expansion medium containing different concentrations of acetaminophen (Wako), troglitazone (Wako) and amiodaron (FUJIFILM Wako Pure Chemical) for 7 days. As a control group, PHHs (lot OHO; Celsis) cultured with hepatocyte culture medium (HCM; Lonza) for 48 h after plating on a 96-well plate were exposed to different concentrations of acetaminophen (Wako), troglitazone (Wako) and amiodaron (FUJIFILM Wako Pure Chemical) for 7 days. Cell viability was evaluated using a Cell Counting Kit-8 (Dojindo Laboratories) based on a WST-8 assay according to the manufacturer's instructions. The absorbance at 450 nm was determined by a multiplate reader (Bio-Rad). Cell viability was calculated as a percentage of the control (DMSO-treated group) viability, which was taken as 100%.

Tong Y., Ueyama-Toba Y., Yokota J., Matsui H., Kanai M, & Mizuguchi H. (2024). Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research. Scientific Reports, 14, 10846.

+ Open protocol

+ Expand

Comprehensive Pharmacological Compound Database

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amoxicillin, atropine, carbamazepine, dicloxacillin, digoxin, erythromycin, estradiol, furosemide, halothane, streptomycin, ticlopidine and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Allopurinol, azathioprine, diclofenac, diphenhydramine, flutamide, ibuprofen, imipramine, indomethacin, isoniazid, kanamycin, ketoconazole, metronidazole, nifedipine, phenobarbital, phenytoin, pioglitazone, sulfamethoxazole, troglitazone and valproic acid were purchased from Wako Pure Chemical (Osaka, Japan). Primidone was purchased from the Tokyo Chemical Industry (Tokyo, Japan). Primers were commercially synthesized at Life Technologies (Carlsbad, CA, USA). HaCaT cells were purchased from CLS Cell Lines Service (Eppelheim, Germany). CnT-Prime (CnT-PR) Epithelial Culture Medium and CnT-Prime 2D Diff (CnT-PR-D) Epithelial Culture Medium were from CELLnTEC Advanced Systems (Bern, Switzerland). All other chemicals and solvents were of analytical grade or the highest grade commercially available.

Hirashima R., Itoh T., Tukey R.H, & Fujiwara R. (2017). Prediction of drug-induced liver injury using keratinocytes. Journal of applied toxicology : JAT, 37(7), 863-872.

+ Open protocol

+ Expand

Luciferase Assay for PPARγ Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

We performed a luciferase reporter assay according to our previous report [15 (link)]. Briefly, monkey CV-1 kidney cells (American Type Culture Collection, USA) reached 90% confluence on a 100-mm culture plate were transfected with a reporter vector (p4 × UASg-tk-Luc), an expression vector for a chimera protein for GAL4 DNA-binding domain and human PPARγ ligand-binding domain (pM-hPPARγ), and an internal control vector (pRL-CMV) for 4 h. These vectors were kindly provided by Prof. Teruo Kawada and Dr. Tsuyoshi Goto, Kyoto University. After transfection, cells were seeded at 5.00 × 10³ cells/well on 96 well plates and treated with medium containing 5 µM troglitazone (FUJIFILM Wako) dissolved in DMSO and either each concentration (0, 10, 20, 25, 30, 40 µg/mL) of YMO dissolved in ethanol or 5 µM GW9662 (FUJIFILM Wako) dissolved in ethanol for 24 h. Luciferase reporter assay was performed using Dual-Glo luciferase assay system (Promega, Madison, WI, USA).

Takahashi A., Ishizaki M., Kimira Y., Egashira Y, & Hirai S. (2021). Erucic Acid-Rich Yellow Mustard Oil Improves Insulin Resistance in KK-Ay Mice. Molecules, 26(3), 546.

+ Open protocol

+ Expand

Adipogenic Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified Eagle’s medium (DMEM), insulin, dexamethasone, troglitazone, protease inhibitor cocktail III, and diamidinophenylindole (DAPI) were purchased from Wako, Osaka, Japan. Bovine serum (BS) and Alexa fluor 488-acetylated LDL were purchased from Life Technologies, Carlsbad, CA. DiI–VLDL was purchased from Kalen Biomedical, Germantown, MD. Blasticidin and fetal bovine serum (FBS) were purchased from Biowest, Nuaille, France. Isobutylmethylxanthine (IBMX), CO-releasing molecule 3 (CO-RM3), FLAG peptide, anti-FLAG antibody-conjugated agarose and [¹³C₆]-glucose were purchased from Sigma-Aldrich, St. Louis. 2-deoxyglucose (2-DG) was purchased from the Tokyo chemical industry, Tokyo, Japan.

Furuhata R., Kabe Y., Kanai A., Sugiura Y., Tsugawa H., Sugiyama E., Hirai M., Yamamoto T., Koike I., Yoshikawa N., Tanaka H., Koseki M., Nakae J., Matsumoto M., Nakamura M, & Suematsu M. (2020). Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Communications Biology, 3, 479.

+ Open protocol

+ Expand

PPAR Agonist and Antagonist Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

YMO was provided from Kewpie Corporation (Tokyo, Japan). A synthetic PPARγ agonist, troglitazone, and a synthetic PPARγ antagonist, GW9662, were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ethanol and dimethyl sulfoxide (DMSO) were also obtained from FUJIFILM Wako Pure Chemical Corporation.

Takahashi A., Ishizaki M., Kimira Y., Egashira Y, & Hirai S. (2021). Erucic Acid-Rich Yellow Mustard Oil Improves Insulin Resistance in KK-Ay Mice. Molecules, 26(3), 546.

+ Open protocol

+ Expand

Srebp-1c-Deficient Mouse Embryonic Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male and female Srebp‐1c^+/− mice were crossed, and MEFs were prepared from pregnant females. Each 13‐ to 15‐day‐old embryo was dissected from the uterus and washed with PBS. After removal of the head, tail, limbs, and blood‐enriched organs, the remaining tissue was washed with PBS, minced, and trypsinized at 37 °C for 10 min. After inactivation of trypsin by adding fetal bovine serum (FBS; Sigma‐Aldrich, St. Louis, MO, USA), MEFs were separated by filtration through a cell strainer. Cells were cultured and passaged in MEM High Glucose (Wako) with 10% FBS, 1% penicillin and streptomycin (Sigma), and 0.1 μM 2‐mercaptoethanol (Sigma). To induce adipocyte differentiation, MEFs were cultured until confluence. At confluence, maintenance medium was changed to MEF differentiation medium, containing 500 μM 3‐isobutyl‐1‐methylxanthine (Sigma), 1 μM dexamethasone (Sigma), 10 μg mL⁻¹ insulin (Sigma), and 100 μM troglitazone (Wako). The differentiation medium was changed every other day and used for quantitative real‐time RT–PCR, Western blotting, and ChIP assay. KO‐MEFs were also infected with retrovirus expressing Srebp‐1c.

Fujii N., Narita T., Okita N., Kobayashi M., Furuta Y., Chujo Y., Sakai M., Yamada A., Takeda K., Konishi T., Sudo Y., Shimokawa I, & Higami Y. (2017). Sterol regulatory element‐binding protein‐1c orchestrates metabolic remodeling of white adipose tissue by caloric restriction. Aging Cell, 16(3), 508-517.

+ Open protocol

+ Expand

DILI Compound Exposure and LCA Pre-treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

DILI drug compounds were dissolved in DMSO to a final concentration of 1% in cell culture medium. Each drug was exposed in three doses for 24 or 72 h with or without LCA 1-h pre-treatment. The following DILI drugs used in this study were categorized into two types: CM-DILI drugs—cyclosporin A (Wako), carbamazepine (Tokyo Chemical Industry), ketoconazole (Sigma-Aldrich), troglitazone (Wako), bosentan (Toronto Research Chemicals), flutamide (LKT Laboratories), diclofenac (Wako), amoxicillin–clavulanate (amoxicillin, Wako; clavulanate, Matrix Scientific) and methapyrilene (Toronto Research Chemicals); and HC-DILI drugs—tacrine (Cayman), tolcapone (Tocris Bioscience) and acetaminophen (Toronto Research Chemicals).

Koido M., Kawakami E., Fukumura J., Noguchi Y., Ohori M., Nio Y., Nicoletti P., Aithal G.P., Daly A.K., Watkins P.B., Anayama H., Dragan Y., Shinozawa T, & Takebe T. (2020). Polygenic architecture informs potential vulnerability to drug-induced liver injury. Nature medicine, 26(10), 1541-1548.

+ Open protocol

+ Expand

Human Microsomes Compound Metabolism Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pooled human microsomes (n = 50, mixed gender) were purchased from Sekisui XenoTech, LLC (Kansas City, KS, USA). NADPH-generating system was purchased from BD Bioscience (Franklin Lakes, NJ, USA). Troglitazone, clozapine, indinavir and acetonitrile (MeCN) were purchased from Wako Pure Chemicals (Osaka, Japan). Nefazodone hydrochloride, buspirone hydrochloride, ciprofloxacin, diclofenac sodium, ketoconazole and carbamazepine were purchased from Sigma-Aldrich (St Louis, MO, USA). Quetiapine fumarate was purchased from Toronto Research Chemicals (North York, Canada). Rimonabant was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). [ 35 S]Cys, [ 14 C]KCN, Ultima FLO-M, and Deepwell LumaPlate-96 were purchased from PerkinElmer Life and Analytical Science (Boston, MA, USA). XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm × 150 mm and Sep-Pak tC18 96-well µElution Plates were purchased from Waters Corporation (Milford, MA, USA). Formic acid (FA) and dimethyl sulfide (DMSO) were purchased from Nacalai Tesque (Kyoto, Japan).

Kakutani N., Nanayama T, & Nomura Y. (2019). Novel risk assessment of reactive metabolites from discovery to clinical stage. The Journal of toxicological sciences, 44(3).

+ Open protocol

+ Expand

Lipid Membrane Permeability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Troglitazone, pioglitazone, and rhodamine-123 were purchased from Wako Pure Chemical Industries, Ltd.
(Osaka, Japan). Phenformin was from Sigma-Aldrich Corp. (St. Louis, MO, USA), and calcein-AM was from Nacalai Tesque, Inc. (Kyoto, Japan). Hoechst 33342 was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), and bovine heart CL was from Avanti Polar Lipids (Alabaster, AL, USA). Other general reagents were of analytical grade and obtained from conventional commercial sources.

Segawa M., Sekine S., Sato T, & Ito K. (2018). Increased susceptibility to troglitazone-induced mitochondrial permeability transition in type 2 diabetes mellitus model rat. The Journal of toxicological sciences, 43(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!