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Sx 20mv stopped flow apparatus

Manufactured by Applied Photophysics
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The SX-20MV stopped-flow apparatus is a laboratory instrument designed to rapidly mix and analyze chemical or biological samples. It is capable of measuring kinetic reactions that occur on a millisecond timescale. The core function of this instrument is to provide a controlled and reproducible environment for rapid mixing and detection of sample changes over time.

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Lab products found in correlation

Kv500 filter, by Schott (3 mentions)

Close spelling variants of this product

Stopped-flow apparatus (20 citations) SX-20MV (17 citations)

3 protocols using sx 20mv stopped flow apparatus

1

Rapid Kinetic Binding of RF1-GAQ

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Rapid kinetic experiments were performed on an SX-20MV stopped-flow apparatus (Applied Photophysics, Leatherhead, UK), by rapidly mixing equal volumes (60 µl) of reactants at 37 °C in buffer A. Binding of RF1-GAQ was monitored by mixing termination complex labelled with fluorescein (50 nM) with RF1-GAQQSY9 (300 nM). Fluorescein was excited at 470 nm and monitored after passing a KV500 filter (Schott, Mainz, Germany).
Graf M., Huter P., Maracci C., Peterek M., Rodnina M.V, & Wilson D.N. (2018). Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1. Nature Communications, 9, 3053.
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2

Rapid Kinetics of Ribosome Release

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Rapid kinetics measurements were performed on an SX-20MV stopped-flow apparatus (Applied Photophysics, Leatherhead, UK). Experiments were performed by rapidly mixing equal volumes (60 μl) of f[3H]Met-tRNAfMet(Flu)-carrying PreHC (0.05 μM), preincubated with Api137 for 2 min at room temperature and RF1Qsy (0.15 μM) at 37°C. Fluorescein was excited at 470 nm and fluorescence emission was monitored after passing a KV500 filter (Schott). Time courses were evaluated by fitting using exponential functions by GraphPad Prism software. Dissociation rates (koff) were determined by chase experiments: PreHCflu (0.05 μM) was preincubated with 0.15 μM RF1Qsy to generate PostHCflu in the absence or presence of 1 μM Api137. PreHC was then rapidly mixed with a 10-fold excess of unlabeled RF1 and RF3·GTP (1 mM); pyruvate kinase (0.1 mg/ml), and phosphoenol pyruvate (3 mM) were present in both syringes. The increase of fluorescence upon dissociation of RF1Qsy was monitored as described above.
Florin T., Maracci C., Graf M., Karki P., Klepacki D., Berninghausen O., Beckmann R., Vázquez-Laslop N., Wilson D.N., Rodnina M.V, & Mankin A.S. (2017). An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome. Nature structural & molecular biology, 24(9), 752-757.
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3

Rapid Kinetics of Ribosome Release

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Rapid kinetics measurements were performed on an SX-20MV stopped-flow apparatus (Applied Photophysics, Leatherhead, UK). Experiments were performed by rapidly mixing equal volumes (60 μl) of f[3H]Met-tRNAfMet(Flu)-carrying PreHC (0.05 μM), preincubated with Api137 for 2 min at room temperature and RF1Qsy (0.15 μM) at 37°C. Fluorescein was excited at 470 nm and fluorescence emission was monitored after passing a KV500 filter (Schott). Time courses were evaluated by fitting using exponential functions by GraphPad Prism software. Dissociation rates (koff) were determined by chase experiments: PreHCflu (0.05 μM) was preincubated with 0.15 μM RF1Qsy to generate PostHCflu in the absence or presence of 1 μM Api137. PreHC was then rapidly mixed with a 10-fold excess of unlabeled RF1 and RF3·GTP (1 mM); pyruvate kinase (0.1 mg/ml), and phosphoenol pyruvate (3 mM) were present in both syringes. The increase of fluorescence upon dissociation of RF1Qsy was monitored as described above.
Florin T., Maracci C., Graf M., Karki P., Klepacki D., Berninghausen O., Beckmann R., Vázquez-Laslop N., Wilson D.N., Rodnina M.V, & Mankin A.S. (2017). An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome. Nature structural & molecular biology, 24(9), 752-757.
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