N latex cystatin c

Manufactured by Siemens

Sourced in Germany, United States

N Latex Cystatin C is a diagnostic product used to quantitatively determine the concentration of cystatin C in human serum and plasma samples. It is a laboratory equipment designed for in vitro diagnostic use.

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Bnii nephelometer, by Siemens (7 mentions) Bn 2, by Siemens (4 mentions) Vitros analyzer, by Johnson & Johnson (2 mentions) Hem 780n3, by Omron (2 mentions) Dimension xpand plus system, by Siemens (2 mentions) Xpand plus system, by Siemens (2 mentions) Behring nephelometer 2 analyzer, by Siemens (2 mentions) Sonos 5500 ultrasound system, by Hewlett-Packard (1 mentions) S3 transducer, by Hewlett-Packard (1 mentions) Vitros crea, by Ortho Clinical Diagnostics (1 mentions) Modular p analysis system, by Hitachi (1 mentions) Cystatin c, by Siemens (1 mentions) C reactive protein (crp), by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions) Ca 7000, by Sysmex (1 mentions) Dimension vista 1500, by Siemens (1 mentions) Array 360 ce protein analyzer, by Beckman Coulter (1 mentions) Vitros 950irc instrument, by Johnson & Johnson (1 mentions) Ektachem 700, by Kodak (1 mentions)

Spelling variants of this product

N Latex Cystatin (4 citations) Cystatin C (4 citations) N Latex Cystatin C assay (4 citations) N Latex Cystatin C on the BNII (3 citations) N Latex Cystatin C kit (2 citations)

32 protocols using n latex cystatin c

Cardiovascular Biomarkers in Aldosteronism

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The aldosterone concentration was measured by radioimmunoassay using a commercial kit (Aldosterone Maia Kit, Adaltis Italia S.P.A., Bologna, Italy)22 (link) and PRA was measured by the generation of angiotensin I in vitro using a commercially available radioimmunoassay kit (DiaSorin, Stillwater, MN, USA)19 (link). The amount of daily protein loss was defined as the urinary microalbumin-to-creatinine ratio (mg/mg); the cardiovascular marker cystatin C11 (link) was measured using a particle-enhanced immunonephelometric assay (N Latex Cystatin C; Siemens, Berlin, Germany) with a nephelometer (BNII; Siemens)23 (link). The pulse wave velocity (PWV) was measured with the subject in a supine position after a 15-min rest using an automatic waveform analyzer (Colin VP-2000, Omeron Inc., Japan), as previously reported24 (link).
A Hewlett-Packard Sonos 5500 ultrasound system equipped with an S3 transducer was used for Echocardiography, including two-dimensional, M-mode and Doppler ultrasound recordings. Left atrial diameter and left ventricular ejection fraction (M-mode) were measured via the parasternal long-axis view, as in our previous report25 (link). Left ventricular mass (LVM) index (LVMI) was calculated according to the method of Devereux et al.26 (link).

Wu V.C., Huang K.H., Peng K.Y., Tsai Y.C., Wu C.H., Wang S.M., Yang S.Y., Lin L.Y., Chang C.C., Lin Y.H., Lin S.L., Chu T.S, & Wu K.D. (2015). Prevalence and clinical correlates of somatic mutation in aldosterone producing adenoma-Taiwanese population. Scientific Reports, 5, 11396.

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Estimating GFR from Serum Cystatin C

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In the ULSAM cohort, GFR was estimated from serum cystatin C using latex‐enhanced reagent (N Latex Cystatin C and a BN ProSpec analyser, Siemens) according to the formula: estimated GFR = 77.24*cystatin C¯^1·2623. In the PIVUS cohort, GFR was estimated using Gentian reagents (Moss, Norway) according to the formula: estimated GFR = 79.901*cystatin C¯^1·4389 in PIVUS. Both formulas for the calculation of GFR are closely correlated with plasma iohexol clearance 25, 26.

Sjöberg B., Qureshi A.R., Heimbürger O., Stenvinkel P., Lind L., Larsson A., Bárány P, & Ärnlöv J. (2015). Association between levels of pentraxin 3 and incidence of chronic kidney disease in the elderly. Journal of Internal Medicine, 279(2), 173-179.

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Renal Function Assessment via Biomarkers

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Renal function was assessed by measuring sCr and CysC. Serial blood samples were obtained at 0, 6, 12, 24, 48, and 72 hours from presentation to the ED. After centrifugation at 3,600 rpm for 15 minutes, serum samples were stored at −20°C until used. CysC was measured by particle enhanced immunonephelometric assay (N Latex Cystatin C, Siemens, Marburg, Germany), and its measuring range was 0.76 mg/L to 1.44 mg/L. The results of CysC were blinded to the medical staffs during the study and did not affect the management of patients. sCr was measured by enzymatic assay (Vitros Crea; Ortho Clinical Diagnostics, High Wycombe, UK), and its normal range was 0.8 to 1.5 mg/dL. The biomarker assays were performed following manufacturer's instructions. As recommended by Kidney Disease Outcome Quality Initiative (KDOQI) guidelines, eGFR was calculated using the modification of diet in renal disease (MDRD) formula [30 (link)–34 (link)].

Bongiovanni C., Magrini L., Salerno G., Gori C.S., Cardelli P., Hur M., Buggi M, & Di Somma S. (2015). Serum Cystatin C for the Diagnosis of Acute Kidney Injury in Patients Admitted in the Emergency Department. Disease Markers, 2015, 416059.

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Plasma Cystatin C Measurement Protocols

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Plasma Cystatin C was measured by a fully automated PETIA. The reagents were obtained from DAKO (Dako A/S, Glostrup, Denmark) and the determination was performed on the Hitachi Modular P analysis system. The total analytical imprecision was 2.1% for a control sample at a concentration of 1.0 mg/L and 1.7% for a control sample at 4.0 mg/L. Reference range: 0.55 to1.15 mg/L for age 1 to 50 years and 0.63 to 1.44 mg/L for age >50 years. Reagent catalogue number: 06600239190—DAKO. The Tina-quant a Cystatin C is a latex particle-enhanced immunoturbidimetric assay. Roche cobas 6000, MODULAR ANALYTICS SWA and COBAS INTEGRA instruments were included in the study. Method comparison studies were carried out against 2 turbidimetric methods (Dako Cystatin C, Gentian Cystatin C), and 1 nephelometric method (Siemens N-Latex Cystatin C).
Routine laboratory investigations were performed according to our local standards.

Zakaria M., Hassan T., Refaat A., Fathy M., Hashem M.I., Khalifa N., Ali A.A., Elhewala A., Ramadan A, & Nafea A. (2022). Role of serum cystatin C in the prediction of acute kidney injury following pediatric cardiac surgeries: A single center experience. Medicine, 101(49), e31938.

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Serum Cystatin C, GFR, and Microalbuminuria

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Serum cystatin C was measured from fasting blood samples by means of a particle-enhanced immunonephelometric assay (N Latex Cystatin C, Siemens Healthcare Diagnostics, Inc., Tarrytown, NY, USA) using a BN II nephelometer (Siemens Healthcare Diagnostics, Inc., Tarrytown, NY, USA).[19 (link)] GFR was estimated using serum creatinine and cystatin C levels, per the Chronic Kidney Disease-Epidemiology Collaboration (CKD-EPI) equation.[20 (link)] The urine spot microalbumin/creatinine ratio (microgram/milligram creatinine) was measured using nephelometry, with a ratio ≥30 mg/g indicative of the presence of microalbuminuria.[19 (link)]
Other laboratory parameters for cerebrovascular risk factors, including total cholesterol (TC, mg/dL), low-density lipoprotein (LDL, mg/dL) cholesterol, hemoglobin A1c (HbA1c, %), and the inflammation marker C-reactive protein (CRP, mg/dL), were also measured at baseline and the end of follow-up.[30 (link)] Changes in these parameters between the baseline and the end of follow-up were also calculated, and designated as ΔTC, ΔLDL, ΔHbA1c, and ΔCRP.

Lee W.J., Jung K.H., Ryu Y.J., Kim J.M., Lee S.T., Chu K., Kim M., Lee S.K, & Roh J.K. (2017). Cystatin C, a potential marker for cerebral microvascular compliance, is associated with white-matter hyperintensities progression. PLoS ONE, 12(9), e0184999.

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Aldosterone, PRA, and Biomarker Measurements

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The concentration of aldosterone was measured by radioimmunoassays with commercial kits (Aldosterone Maia Kit, Adaltis Italia S.p.A., Bologna, Italy). The lowest detectable concentration of aldosterone is 10.0 pg/mL. The normal range of aldosterone is 70–350 pg/mL in the upright position. PRA was measured as the generation of angiotensin I in vitro using a commercially available radioimmunoassay kit (Stillwater, MN, USA). The normal range of PRA was 2.63 ± 1.32 ng/mL/ h in the upright position. The mean (standard deviation [SD]) intra and interassay coefficients of variation (CVs) for the PRA assay were 1.9 (5.0%) and 4.5 (5.2%) respectively.
The plasma levels of TNF-α, CRP (R&D Systems, Minneapolis, MN) and NT-proBNP (USCN Life Science, Wuhan, P.R. China) were measured using the commercially available enzyme-linked immunosorbent assay kits according to the manufacturer’s instructions. Urine albumin excretion was defined as the urinary albumin-to-creatinine ratio (mg/mg) determined using the first morning void. Cystatin C was measured using a particle-enhanced immunonephelometric assay (N Latex Cystatin C; Siemens, Berlin, Germany) with a nephelometer (BNII; Siemens).

Wu C.H., Yang Y.W., Hung S.C., Tsai Y.C., Hu Y.H., Lin Y.H., Chu T.S., Wu K.D, & Wu V.C. (2015). Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone. Scientific Reports, 5, 15297.

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Serum Biomarkers Measurement Protocol

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Blood samples were collected from the antecubital vein in all enrolled patients. The collected serum samples were stored at -20 C° until instrumental analysis.
Serum CysC was measured by a particle-enhanced immuno-nephelometric assay (N Latex Cystatin C, BN ProSpec nephelometer, Siemens Healthcare Diagnostics).
sCrea and hs-CRP measurements were performed by an automated method using Dimension VISTA 1500 (Siemens Healthcare Diagnostics, Milano, Italy).
Fibrinogen was quantified by phototurbimetric method (Ca 7000 Sysmex, Japan).

Rovella V., Marrone G., Dessì M., Ferrannini M., Toschi N., Pellegrino A., Casasco M., Di Daniele N, & Noce A. (2018). Can Serum Cystatin C predict long-term survival in cardiac surgery patients?. Aging (Albany NY), 10(3), 425-433.

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Serum Creatinine and Cystatin-C Comparison

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Table 1 showed the different equations used for comparison in this study. All patients had their serum creatinine and serum cystatin-C blood test withdrawn during the peripheral venous access insertion. Measurement of serum creatinine was performed using enzymatic creatinine assay based on the enzymatic reaction [10 ] with normal adult reference range of 53–97 μmol/L for male and 44–71 μmol/L for female. The creatinine values were adjusted to isotope dilution mass spectrometry (IDMS) traceable assay. Measurement of serum cystatin-C was performed at an independent pathology laboratory outside the hospital using an automated particle-enhanced immune-nephelometry method with normal reference range of 0.85 mg/l or less using N Latex Cystatin-C from Siemens Healthcare Diagnostics Products GmbH (Germany). N Latex Cystatin-C values were then converted to eGFR based on the Hoek equation. The adjusted Hoek equation to calculate the eGFR is GFR (ml/min/1.73 m²) = −4.32 + 80.35/cys-C [11 (link)].

Jalalonmuhali M., Elagel S.M., Tan M.P., Lim S.K, & Ng K.P. (2018). Estimating Renal Function in the Elderly Malaysian Patients Attending Medical Outpatient Clinic: A Comparison between Creatinine Based and Cystatin-C Based Equations. International Journal of Nephrology, 2018, 3081518.

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Urinary Biomarkers and Cystatin C Analysis

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Urinary albumin concentration was determined by nephelometry, using the Array 360 CE Protein Analyzer (Beckman Instruments Inc); the lowest detectable level was 0.2 mg/dL. Urinary creatinine was measured by the Jaffé rate method using the Vitros 950IRC instrument (Johnson & Johnson Clinical Diagnostics Inc); the range was 0.05–16.5 mg/dL, with a coefficient of variation range of 2.5%–2.9%. Spot urine ACR was calculated. Cystatin C was measured using a BN II nephelometer (Siemens; www.medical.siemens.com) with a particle-enhanced immunonephelometric assay (N Latex Cystatin C, Siemens) on fasting plasma specimens stored at −70°C. The intra-assay coefficient of variation for cystatin C ranged from 2.0% to 2.8%.

Garimella P.S., Ix J.H., Katz R., Shlipak M.G., Criqui M.H., Siscovick D.S., Kramer H., Sibley C.T, & Sarnak M.J. (2014). Association of Albumin-Creatinine Ratio and Cystatin C With Change in Ankle-Brachial Index: The Multi-Ethnic Study of Atherosclerosis (MESA). American journal of kidney diseases : the official journal of the National Kidney Foundation, 65(1), 33-40.

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Measurement of Kidney Biomarkers

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Urea was determined by an enzymatic method (UREA/BUN Roche/Cobas; Roche Diagnostics, Mannheim, Germany). Creatinine was measured with a rate-blanked creatinine/Jaffé method (CREA Roche/Hitachi automated analysis for Hitachi 917, Roche Diagnostics, Mannheim, Germany). β2M was measured with an immune-enzymic method (AxSym ß2-Microglobulin, Abbott, Wiesbaden, Germany; mean reference value 0.99 ± 0.16 mg/L). Cys was measured with a particle enhanced immune-nephelometric method (N Latex Cystatin C, Siemens Healthcare, Erlangen, Germany; reference intervals 0.53–0.95 mg/L). βTP was measured with a particle enhanced immune-nephelometric method (N Antiserum to human βTP, Siemens Healthcare, Erlangen, Germany). Reference intervals, calculated in our laboratory, were 0.37–0.77 mg/L in men, and 0.40–0.70 mg/L in women [14 (link)].

Donadio C., Tognotti D., Caponi L, & Paolicchi A. (2017). β-trace protein is highly removed during haemodialysis with high-flux and super high-flux membranes. BMC Nephrology, 18, 68.

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