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Rs 2000 small animal irradiator

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The RS-2000 small animal irradiator is a compact and versatile laboratory device designed to precisely deliver controlled doses of radiation for small animal studies. It features a self-shielded cabinet and a radiation source for controlled irradiation of samples.

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Lab products found in correlation

Matrigel, by Corning (2 mentions) Stem pro accutase solution, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Stempro nsc sfm, by Thermo Fisher Scientific (1 mentions) Agilent seahorse xf96 cell culture microplate, by Agilent Technologies (1 mentions)

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RS-2000 irradiator (30 citations)

9 protocols using rs 2000 small animal irradiator

1

Targeted Tumor Irradiation in Xenografts

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CUHN013 xenograft-bearing mice were irradiated using an RS-2000 small animal irradiator (Rad Source Technologies, Suwanee, GA), calibrated to deliver 1.15Gy X-ray radiation per minute. Mice were anesthetized via IP injection with a ketamine (60mg/kg)/xylazine (8mg/kg) solution, then positioned beneath a lead/cadmium shield, designed to allow radiation to penetrate only the flank tumors. A 3Gy dose was administered to each tumor, and the mice were allowed to recover for 24 hours before tissue collection.
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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2

Medulloblastoma Treatment in Mice

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SHH-MB mice at advanced symptomatic stages (domed head, weight loss, ataxia) were treated with whole-body XRT using a RS2000 small animal irradiator (Rad Source) at the indicated doses (0, 0.25, 1 or 2 Gy) alone, with free vismodegib alone, with nanoparticle-encapsulated vismodegib (FiVis or DexVis) alone or in combinations as indicated in individual figure legends. When used in combination, drugs were administered via intraperitoneal injection 2 h following XRT administration and assayed at the indicated time points for immunoblot and RT–qPCR studies. Treatment for survival studies was given on alternate days for a total of eight doses starting on day 0, with all treatments in all experimental groups ending on day 16.
Tylawsky D.E., Kiguchi H., Vaynshteyn J., Gerwin J., Shah J., Islam T., Boyer J.A., Boué D.R., Snuderl M., Greenblatt M.B., Shamay Y., Raju G.P, & Heller D.A. (2023). P-selectin-targeted nanocarriers induce active crossing of the blood–brain barrier via caveolin-1-dependent transcytosis. Nature Materials, 22(3), 391-399.
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3

Characterization of Patient-Derived Glioblastoma Cell Lines

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Twelve patient-derived glioblastoma cell lines were kindly provided by the QIMR Berghofer Medical Research Institute (Brisbane, Australia). The cell lines are fully characterised with publicly available molecular and patient data, published by Stringer et al. [13 (link)]. Cells were grown as adherent monolayers in Matrigel® (Corning®, Corning, NY, USA)-coated tissue culture flasks in StemPro® NSC SFM (GibcoTM, Waltham, MA, USA) containing 100 I.U./mL penicillin and 100 µg/mL streptomycin (GibcoTM, Waltham, MA, USA), and incubated at 37 °C in 5% CO2/95% humified air. Cells were passaged using StemPro® Accutase® solution (GibcoTM, Waltham, MA, USA) for detachment of adherent cells. TMZ was purchased from Sigma-Aldrich (Burlington, MA, USA), aliquoted in dimethyl sulfoxide (DMSO) (100 mM) and stored between 2 and 8 °C. RT was delivered using a medical linear accelerator (LINAC) at GenesisCare, Gateshead NSW (Australia) or RS-2000 Small Animal Irradiator (Rad Source, Buford, GA, USA).
Lozinski M., Bowden N.A., Graves M.C., Fay M., Day B.W., Stringer B.W, & Tooney P.A. (2022). Transcriptomic Profiling of DNA Damage Response in Patient-Derived Glioblastoma Cells before and after Radiation and Temozolomide Treatment. Cells, 11(7), 1215.
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4

Bone Marrow Cell Engraftment Assay

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BM cells were collected from CD45.1+ wildtype, CD45.2+-Sh2b3+/+ and CD45.2+- Sh2b3E372K/E372K mice. Cells from CD45.1+ wildtype and CD45.2+-Sh2b3+/+ or CD45.2+- Sh2b3E372K/E372K mice were mixed at 1:1 ratio, respectively, and 2 × 106 BM cell mix injected into the tail vein of each corresponding Rag1tm1Mom (hereinafter referred to as Rag-/-) mouse that had received 500 cGy radiation from the RS2000 small animal irradiator (Rad Source Technologies). Injected mice were allowed 10 weeks for BM reconstitution before analysis.
Zhang Y., Morris R., Brown G.J., Lorenzo A.M., Meng X., Kershaw N.J., Kiridena P., Burgio G., Gross S., Cappello J.Y., Shen Q., Wang H., Turnbull C., Lea-Henry T., Stanley M., Yu Z., Ballard F.D., Chuah A., Lee J.C., Hatch A.M., Enders A., Masters S.L., Headley A.P., Trnka P., Mallon D., Fletcher J.T., Walters G.D., Šestan M., Jelušić M., Cook M.C., Athanasopoulos V., Fulcher D.A., Babon J.J., Vinuesa C.G, & Ellyard J.I. (2024). Rare SH2B3 coding variants in lupus patients impair B cell tolerance and predispose to autoimmunity. The Journal of Experimental Medicine, 221(4), e20221080.
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5

Targeted Tumor Irradiation in Xenografts

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CUHN013 xenograft-bearing mice were irradiated using an RS-2000 small animal irradiator (Rad Source Technologies, Suwanee, GA), calibrated to deliver 1.15Gy X-ray radiation per minute. Mice were anesthetized via IP injection with a ketamine (60mg/kg)/xylazine (8mg/kg) solution, then positioned beneath a lead/cadmium shield, designed to allow radiation to penetrate only the flank tumors. A 3Gy dose was administered to each tumor, and the mice were allowed to recover for 24 hours before tissue collection.
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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6

Irradiation Impact on Salivary Gland ATP

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Primary cells isolated from parotid salivary glands were plated on an Agilent Seahorse XF96 Cell Culture Microplate (Part No. 101085-004, Agilent Technologies, Cedar Creek, TX) at a seeding density of 150,000 cells/well and cultured prior to irradiation at 24 h or 48 h before running the Seahorse ATP Rate Assay. Cells were exposed to a single dose of 5 Gy irradiation (X-ray, RS 2000 Small Animal Irradiator, Rad Source). The untreated cells were shielded with > 6 mm lead.
Buss L.G., Rheinheimer B.A, & Limesand K.H. (2024). Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands. Scientific Reports, 14, 845.
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7

Radiotherapy and Immunotherapy Combination

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Once tumors reached ~150 mm3 (day −17), mice were randomized to different groups before treatment (n = 7–8). Mice were anesthetized with inhaled isoflurane and IP dexmedetomidine (5 μg/g body weight) before RT treatment. RT was administered using the RS 2000 small animal irradiator (160 kV, 25 mA and mean beam as 2 Gy/min Rad Source; Brentwood, TN) at 8 Gy for 3 consecutive days36 (link) before immunotherapy treatment (days −3, −2, and −1). A rigid exposed-flank shield (Precision X-ray Inc) and a flexible lead layer were used to shield the mice from irradiation, exposing only the tumor. After RT, atipamezole was administered to reverse dexmedetomidine anesthesia. RT-treated mice were given Baytril wafers and subcutaneous fluid daily for health maintenance.
IP treatment groups were administered with either PBS (control) or a combination of CD40 and PDL1 (100 μg each in 100 μL) on days 0, 2, 4, and 6. NDES was filled with either PBS (control) or ~800 μg lyophilized CD40 and PDL1 and primed for drug release as previously described. Intratumoral implantation occurred on day 0 via a minimally invasive trocar approach similar to brachytherapy seed insertion.26 (link) Because sustained drug release occurs without requiring manipulation upon implantation, no further intervention was performed for the NDES group.
Liu H.C., Viswanath D.I., Pesaresi F., Xu Y., Zhang L., Di Trani N., Paez-Mayorga J., Hernandez N., Wang Y., Erm D.R., Ho J., Susnjar A., Liu X., Demaria S., Chen S.H., Teh B.S., Butler E.B., Xuan Chua C.Y, & Grattoni A. (2020). Potentiating Antitumor Efficacy Through Radiation and Sustained Intratumoral Delivery of Anti-CD40 and Anti-PDL1. International journal of radiation oncology, biology, physics, 110(2), 492-506.
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8

Cranio-Specific Gamma Irradiation in Mice

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Four- to eight-week-old female FVB mice were sedated via 20uL intraperitoneal injection of a ketamine-xylazine mixture (70 mg/kg–10 mg/ml) before irradiation. Mice were then constrained in 50-ml conical tubes and shielded with > 6 mm lead with only their head and neck region exposed to a single dose of 5 Gy irradiation (X-ray, RS 2000 Small Animal Irradiator, Rad Source). Animals were monitored for 1 h following radiation treatment to ensure full recovery from the anesthesia.
Buss L.G., Rheinheimer B.A, & Limesand K.H. (2024). Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands. Scientific Reports, 14, 845.
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9

Twelve Glioblastoma Cell Lines Protocol

Cited 3 times
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Twelve patient-derived glioblastoma cell lines (BAH1, FPW1, JK2, MMK1, RKI1, HW1, PB1, SB2b, SJH1, RN1, MN1, and WK1) were kindly provided by Professor Bryan Day (QIMR Berghofer Medical Research Institute, Brisbane, Australia). The cell lines are fully characterised with publicly available molecular and patient data, published by Stringer et al. [34 (link)]. Cells were grown as adherent monolayers in Matrigel® (Corning®, Corning, NY, USA)-coated tissue culture flasks in serum-free conditions, as previously described [6 (link)]. TMZ was purchased from Sigma-Aldrich (St. Louis, MO, USA), aliquoted in dimethyl sulfoxide (DMSO) (100 mM), and stored between 2–8 °C. RT was delivered using an RS-2000 Small Animal Irradiator (Rad Source, Buford, GA, USA) [35 (link)].
Lozinski M., Lumbers E.R., Bowden N.A., Martin J.H., Fay M.F., Pringle K.G, & Tooney P.A. (2024). Upregulation of the Renin–Angiotensin System Is Associated with Patient Survival and the Tumour Microenvironment in Glioblastoma. Cells, 13(7), 634.
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