Accute tba 40fr

Blood lipids of low-density lipoprotein (LDL), triglycerides (TG), nonestesterified fatty acid (NEFA), total cholesterol (CHO), glycosylated serum protein (GSP), glycated hemoglobin (HbA1c), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and aspartate transaminase (AST) were determined by the automatic biochemical analyzer (Toshiba Accute TBA-40FR, Toshiba Corporation, Tokyo, Japan) for the lipid metabolism and heart function test.
A rat left ventricular tissue homogenate at 10% concentration was prepared with cold PBS buffer for examination of the cardiac troponin T (cTn-T) and pro-brain natriuretic peptic (pro-BNP). The content of nitric oxide (NO) and hydroxyproline (HYP), the activity of nitric oxide synthase (NOS) and ATPase, the content of superoxide dismutase (SOD) and malondialdehyde (MDA), and the activity of glutathione (GSH) in the myocardium were detected by the commercial kit (Jiancheng Biotech Co., Ltd., Nanjing, China).

At the end of the 8-week treatment, blood lipids of triglyceride (TG) and total cholesterol (TC) were detected according to the manufacturer's instruction of the TG and TC test kit (Zhongsheng Beikong Biotechnology Co., Ltd., China) with an automatic analyzer (Toshiba Accute TBA-40FR, Toshiba Corporation, Tokyo, Japan).

The in vivo safety of CHA-SME was evaluated based on body weight, hematological examination, and histopathological examination in mice. Body weight was monitored throughout the experiment. At the end of the in vivo antitumor experiment, peripheral blood was collected for hematological examination using an automatic hematology analyzer (MEK-7222 K; Nihon Kohden, Tokyo, Japan). Additionally, the serum was separated to detect alkaline aminotransferase (ALT), aspartate phosphatase (AST), blood urea nitrogen (BUN), and creatinine (CRE) to evaluate hepatic and renal functions using an automatic biochemical analyzer (Accute TBA-40FR; TOSHIBA, Kawasaki, Japan). Heart, liver, spleen, lung, kidney, stomach, small intestine, and MLNs were collected, fixed in 4% PFA, and subjected to histopathological examination using HE staining.

Serum urea nitrogen (BUN: urease‐GLDH method) and creatinine (enzymatic method) concentrations were measured with an autoanalyzer (Accute TBA‐40FR, Toshiba Medical Systems, Tochigi, Japan). The right kidney from mice given cisplatin or saline was excised, fixed in 10% formalin, embedded in paraffin wax, cut at 3‐μm thickness, stained with hematoxylin and eosin (H‐E), and histopathologically examined. The histological evaluation was conducted in a blinded manner.

The tail of mice was warmed with a light to dilate the blood vessels and wiped with sterilizing cottons containing 70% ethanol. A 1-inch-long 24-gauge needle was used and inserted into 1–2 cm of the distal part of the left tail vein at an angle of approximately 20° under the conscious conditions, and blood (approximately 40 μL/time) was naturally withdrawn by capillary action. After removing the needle, the microcapillary tube containing the blood was centrifuged at 15,000× g for 5 min (MC-201, Hitachi, Tokyo, Japan), and plasma was obtained by cutting a border between the supernatant and cell packages using the file. Serum BUN: urease-GLDH method) and creatinine (enzymatic method) concentrations were measured with an autoanalyzer (Accute TBA-40FR, Toshiba Medical Systems, Tochigi, Japan).