Rabbit anti aldh1l1

The following primary antibodies were used: chicken anti-GFAP (Millipore, catalog no. AB5541; diluted 1:500); rabbit anti-Aldh1l1 (Abcam, catalog no. ab87117; diluted 1:500); rabbit anti-S100β (Abcam, catalog no. ab41548; 1:1000); rabbit anti-NeuN (Cell Signaling Technology, catalog no. 12943; diluted 1:400); rabbit anti-vGlut2 (Synaptic Systems, catalog no. 135403; 1:250).

Tissue cryosections were washed in 1X PBS then blocked for 1h at room temperature (RT) in 1X PBS/0.3% Triton X-100/3% normal donkey or goat serum (blocking solution). Slides were incubated overnight at 4°C with blocking solution containing dilutions of the following antibodies: rabbit anti-ALDH1L1 (Abcam) 1:500; rabbit anti-cleaved caspase-3 (Biocare Medicare) 1:250; rabbit anti-FoxP1 (Abcam Inc.) 1:400; mouse anti-GAD67 (EMD Millipore) 1:5000; rabbit anti-glycine (Millipore) 1:100; chicken anti-MAP2 (Abcam Inc.) 1:5000; rabbit anti-Olig2 (EMD Millipore) 1:250; mouse anti-TUJ1 (Abcam) 1:500. Sections were washed in 1X PBS and incubated for 1h with secondary antibodies conjugated to DyLight 488 or 549 (Jackson Immunoresearch) at a 1:500 dilution. All slides were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) or NeuroTrace fluorescent Nissl stain (Molecular Probes). After staining, sections were mounted with ProLong Gold to preserve the fluorescent signals and imaged using a Leica DM5500B epifluorescence microscope (Leica Microsystems, Exton, PA) or an inverted Zeiss Axio Observer on a PerkinElmer UltraVIEW VoX spinning disk confocal with a Hamamatsu C9100-13 camera and Volocity software.