The determination of TSH levels was carried out using an electrochemiluminescence immunoassay provided by Roche (Mannheim, Germany), with a detectable laboratory range spanning from 0.004 to 100 μIU/mL. The Tg level was assayed using the Elecsys® Tg II kit(Roche Diagnostics, Basilea, Switzerland), with a detection range of 0.04–500.00 ng/mL. The TgAb level was measured using the Elecsys® TgAb kit from Roche Diagnostics, and TgAb levels less than 115 IU/mL were considered negative. Tg was measured with the Elecsys® Tg II kit(Roche Diagnostics, Basilea, Switzerland), with a detection range of 0.04–500.00 ng/mL. Additionally, the TgAb level was assayed using the Elecsys® TgAb kit(Roche Diagnostics, Basilea, Switzerland). TgAb levels falling below 115 IU/mL were deemed negative. Genomic DNA was extracted from the primary tumors and subjected to traditional Sanger sequencing for the identification of BRAFV600E mutations.The BRAFV600E mutation status of primary PTC tumors was determined after surgery.
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