Csfr1cre mice

All animal experiments were performed under the animal protocol (IACUC#: 007158) approved by the Institutional Animal Care & Use Committee at CSMC. Female 8–12 weeks mice were used for all experiments. Wild type (WT; C57Bl/6) and TLR7^−/− mice were purchased from The Jackson Laboratory (Bar Harbor, ME). EGFR^{floxed/floxed} mice were obtained from David Threadgill, PhD at Texas A&M University, and crossbred to CSFR1^Cre mice (The Jackson Laboratory) to generate EGFR^{floxed/floxed}- CSFR1^Cre mice. Mice were bred and kept in a specific pathogen-free condition with 12-h day/night cycles and allowed free access to food and water.

C57BL/6J WT mice (000664; JAX), DsRed mice (006051; JAX; WT C57BL/6J background), CD45.1 mice (002014; JAX; WT C57BL/6J background), CFTR^ΔF508 or CF mice (002515; JAX; C57BL/6J background), and CSFR1-cre mice (029206; JAX; C57BL/6J background) were obtained from the Jackson Laboratory. CFTR^flox/flox (Cftr^tm1Cwr; C57BL/6J background) mice were developed by and obtained from the Cystic Fibrosis Mouse Models Core at Case Western Reserve University. CD45.1/CD45.2 mice were bred by C57BL/6J WT and CD45.1 mice. Mice were fed a standard rodent chow diet and were housed in microisolator cages in a pathogen-free facility in the La Jolla Institute for Immunology or UConn Health. Mice were euthanized by CO₂ inhalation. All experiments followed guidelines of the La Jolla Institute for Immunology and UConn Health Animal Care and Use Committee, and approval for the use of rodents was obtained from the La Jolla Institute for Immunology and UConn Health according to criteria outlined in the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health.