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Teg cholesterol phosphoramidite

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The TEG cholesterol phosphoramidite is a chemical building block used in the synthesis of oligonucleotides. It provides a means to incorporate cholesterol moieties into oligonucleotide sequences.

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Lab products found in correlation

Stearyl phosphoramidite, by Glen Research (2 mentions) Hexaethyleneglycol phosphoramidite, by Glen Research (2 mentions) Mik665, by Selleck Chemicals (2 mentions) Biotin teg phosphoramidite, by Glen Research (1 mentions) 5 amino modifier 5, by Glen Research (1 mentions)

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Phosphoramidites (38 citations)

2 protocols using teg cholesterol phosphoramidite

1

Synthesis and Modification of RNA Oligonucleotides

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2’-O-Me and 2’-F phosphoramidites, universal synthesis columns (MM1-2500-1), and all ancillary RNA synthesis reagents were purchased from Bioautomation (Irving, TX). Symmetrical branching 2’-cyanoethyl, diisopropyl (CED) phosphoramidite (CLP-5215) was obtained from ChemGenes (Wilmington, MA). Cyanine 5 (Cy5) phosphoramidite (10-5915), stearyl phosphoramidite (10-1979), biotin triethyleneglycolyl (TEG) phosphoramidite (10-1955), hexaethyleneglycol phosphoramidite (10-1918), TEG cholesterol phosphoramidite (10-1976), 5’-Amino-Modifier 5 (10-1905), and desalting columns (60-5010) were all purchased from Glen Research (Sterling, VA). PE (phycoerythrin) anti-mouse CD19 antibody (Catalog No. 115508) and APC (allophycocyanin) anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend (San Diego, CA). MIK665 was from Selleck Chem (Houston, TX). All other reagents were purchased from Sigma-Aldrich unless otherwise specified.
Hoogenboezem E.N., Patel S.S., Lo J.H., Cavnar A.B., Babb L.M., Francini N., Gbur E.F., Patil P., Colazo J.M., Michell D.L., Sanchez V.M., McCune J.T., Ma J., DeJulius C.R., Lee L.H., Rosch J.C., Allen R.M., Stokes L.D., Hill J.L., Vickers K.C., Cook R.S, & Duvall C.L. (2024). Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy. Nature Communications, 15, 1581.
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2

Modular RNA Synthesis and Conjugation

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2’-O-Me and 2’-F phosphoramidites, universal synthesis columns (MM1–2500-1), and all ancillary RNA synthesis reagents were purchased from Bioautomation. Symmetrical branching CED phosphoramidite was obtained from ChemGenes (CLP-5215). Cyanine 5 (Cy5) phosphoramidite (10–5915), stearyl phosphoramidite (10–1979), biotin TEG phosphoramidite (10–1955), hexaethyleneglycol phosphoramidite (10–1918), TEG cholesterol phosphoramidite (10–1976), 5’-Amino-Modifier 5 (10–1905), and desalting columns (60–5010) were all purchased from Glen Research. PE anti-mouse CD19 antibody (Catalog No. 115508) and PC anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend. MIK665 was from Selleck Chem. All other reagents were purchased from Sigma-Aldrich unless otherwise specified.
Hoogenboezem E.N., Patel S.S., Cavnar A.B., Lo J.H., Babb L.M., Francini N., Patil P., Colazo J.M., Michell D.L., Sanchez V.M., McCune J.T., Ma J., DeJulius C.R., Lee L.H., Rosch J.C., Allen R.M., Stokes L.D., Hill J.L., Vickers K.C., Cook R.S, & Duvall C.L. (2023). Structural Optimization of siRNA Conjugates for Albumin Binding Achieves Effective MCL1-Targeted Cancer Therapy. bioRxiv.
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