5×105 CD11c+ DC from both culture conditions were incubated in 200 µl of each culture media for 24 hours. Media was collected and analyzed for the presence of the indicated cytokines using a Milliplex MAP Cytokine Immunoassay (Millipore, Billerica, MA).
Milliplex map cytokine immunoassay
Manufactured by Merck Group
The Milliplex MAP Cytokine Immunoassay is a multiplex assay system that allows for the simultaneous quantification of multiple cytokines and chemokines from a single sample. The core function of this product is to provide a platform for the accurate and sensitive detection of various protein analytes in biological samples, such as cell culture supernatants, serum, or plasma.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using milliplex map cytokine immunoassay
1
Cytokine Secretion by Dendritic Cells
2
BDC2.5 CD4+ T Cell Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD4+ T cells were isolated from spleens of BDC2.5 NOD females using CD4 Microbeads (Miltenyi Biotec). 1×105 BDC2.5 CD4+ T cells were co-cultured with 1×104 CD11c+ DC in the presence of the BDC 2.5 mimepitope 1040-55 (10µg/ml) using both culture media in triplicate. On day 3 of co-culture, 50µl media was removed and analyzed for the presence of the indicated cytokines using a Milliplex MAP Cytokine Immunoassay (Millipore). 50 µl fresh media containing 1 µCi[3]H-Thymidine was added to the culture and incubated for 18 hours prior to cell harvest and analysis of Thymidine uptake.
3
Cytokine Secretion by Dendritic Cells
4
BDC2.5 CD4+ T Cell Activation Assay
5
Spleen Cell Cytokine and Proliferation Analysis
6
Spleen Cell Cytokine and Proliferation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty weeks following termination of BM-DC treatment, spleens were collected from remaining non diabetic mice. 1×106 total spleen cells were cultured in HL-1 media in the presence of GAD217-236 peptide (10 µg/ml). On day 4, 50 µl of media was removed and analyzed for the presence of the indicated cytokines using a Milliplex MAP Cytokine Immunoassay (Millipore). 50 µl fresh media containing 1µCi [3]H-Thymidine was added to the culture and incubated for 16 hours prior to cell harvest and analysis of Thymidine uptake.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!