Sirna against c myb

ES2 cells were purchased from ATCC (Manassas, USA) and cultured in McCoy’s 5a medium with 10% FBS in 5% CO₂–humidified incubators at 37°C. siRNA against c-myb was purchased from SCBT (USA). Cisplatin resistant ES2 cells were generated in the laboratory by long term exposure (over four months) of the cells to cisplatin with gradual increase of cisplatin concentration after every 4-5 passages (Supplementary Figure S1). Parental and cisplatin resistant A2780 cells were purchased from Sigma (St Louis, USA) and cultured in RPMI 1640 media with 10% FBS in 5% CO₂–humidified incubators at 37°C. For the routine maintenance and propagation, cisplatin resistant cells were cultured with sub-IC-50 concentrations of cisplatin in the culture media and the cisplatin resistance of these cells was periodically confirmed by evaluating the IC-50 values.

c-myb cloned in pCMV6-XL5 was purchased from Origene and transfected using TurboFectin transfection reagent while siRNA against c-myb was purchased from SCBT (China). Pre-miR-21 and anti-miR-21 reagents were purchased from ThermoFisher (China) and transfected using Dharmafect reagent (Dharmacon, China).