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Spot idea 5.0mp

Manufactured by Zeiss
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The SPOT Idea 5.0mp is a digital camera designed for microscopy applications. It features a 5.0 megapixel image sensor and can capture high-quality images of specimens under a microscope. The camera is compatible with a variety of microscopes and can be used for a range of imaging tasks.

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Lab products found in correlation

Axio observer a1, by Zeiss (3 mentions) Eclipse e800 microscope, by Nikon (3 mentions) Spot pursuit usb, by Zeiss (3 mentions)

3 protocols using spot idea 5.0mp

1

Wide-field Fluorescence Microscopy Workflow

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Wide-field fluorescence microscopy was performed using an Eclipse E800 microscope (Nikon) equipped with epifluorescence optics and a digital camera (Spot Pursuit USB) or an AXIO observer A1 (Zeiss) equipped with epifluorescence optics and a digital camera (SPOT Idea 5.0mp). Image acquisition was done with a 40X air or 60X oil objective. Images were merged using Adobe Photoshop.
Fay M.M., Columbo D., Cotter C., Friend C., Henry S., Hoppe M., Karabelas P., Lamy C., Lawell M., Monteith S., Noyes C., Salerno P., Wu J., Zhang H.M., Anderson P.J., Kedersha N., Ivanov P, & Farny N.G. (2021). Bisphenol A promotes stress granule assembly and modulates the integrated stress response. Biology Open, 10(1), bio057539.
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2

Wide-field Fluorescence Microscopy Imaging

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Wide-field fluorescence microscopy was performed using an Eclipse E800 microscope (Nikon) equipped with epifluorescence optics and a digital camera (Spot Pursuit USB) or an AXIO observer A1 (Zeiss) equipped with epifluorescence optics and a digital camera (SPOT Idea 5.0 mp). Image acquisition was done with a 40X or 100X objective. Images were merged using Adobe Photoshop and fluorescence intensity was quantified using ImageJ.
Kharel P., Fay M., Manasova E.V., Anderson P.J., Kurkin A.V., Guo J.U, & Ivanov P. (2023). Stress promotes RNA G-quadruplex folding in human cells. Nature Communications, 14, 205.
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3

Fluorescence microscopy and RNA folding

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Wide-field fluorescence microscopy was performed using an Eclipse E800 microscope (Nikon) equipped with epifluorescence optics and a digital camera (Spot Pursuit USB) or an AXIO observer A1 (Zeiss) equipped with epifluorescence optics and a digital camera (SPOT Idea 5.0mp). Image acquisition was done with a 40X objective. Images were merged using Adobe Photoshop and fluorescence intensity was quantified using ImageJ.
Poly(A)-selected RNA or in vitro synthesized RNA oligos (Integrated DNA technologies) in 150 mM KCl or LiCl in T10E0.1 buffer (10 mM Tris-HCl pH 7.4 and 0.1 mM EDTA) were heated to 95°C for 5 min and then slowly cooled to room temperature.
Kharel P., Fay M.M., Manasova E.V., Anderson P., Kurkin A.V., Guo J.U., & Ivanov P. (2022). Stress promotes RNA G-quadruplex folding in human cells.
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