Larval tissues were fixed for 20 min in 4% formaldehyde/phosphate-buffered saline (PBS) and immunostained as described (Bello et al. 2003 (link)). The primary antibodies used were rabbit anti-aPKC (1:500; Santa Cruz Biotechnology), mouse anti-Mira (1:50; gift of A. Gould), rat anti-pH3 (1:500; Abcam), chick anti-GFP (1:2000; Abcam), rabbit anti-Dpn (1:100; gift of Y.N. Jan), guinea pig anti-Dpn (1:1000; gift of James Skeath), rat anti-Pros (1:50; gift of F. Matsuzaki), rabbit anti-Ase (1:50; gift of F. Matsuzaki), rat anti-CycE (1:500; gift of Helena Richardson), mouse anti-CycA (1:50; Developmental Studies Hybridoma Bank), mouse or rat anti-Elav (1:100; Developmental Studies Hybridoma Bank), rabbit anti-Insc (1:1000; gift of W. Chia), rabbit anti-Myc (1:100; Santa Cruz Biotechnology), guinea pig anti-Nerfin-1 (1:5000; gift of A. Kuzin), rabbit anti-RFP (1:100; Abcam), and mouse anti-Fib (1:200; Abcam). Secondary goat antibodies conjugated to Alexa488, Alexa568, Alexa650, and Alexa505 (Molecular Probes) were used at 1:200. Images were collected on a Leica SP5 confocal microscope, and all images shown are single sections unless otherwise stated.
Mouse anti mira
Manufactured by Abcam
Mouse anti-Mira is a primary antibody that specifically binds to the Mira protein. Mira is a conserved protein involved in mitotic spindle orientation and asymmetric cell division. This antibody can be used in various applications to detect and study the Mira protein.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti ph3, by Abcam (2 mentions)
Chick anti gfp, by Abcam (2 mentions)
Rabbit anti rfp, by Abcam (2 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Alexa 568, by Thermo Fisher Scientific (1 mentions)
Sp5 confocal microscope, by Leica (1 mentions)
Rabbit anti myc, by Santa Cruz Biotechnology (1 mentions)
Alexa650, by Thermo Fisher Scientific (1 mentions)
Alexa505, by Thermo Fisher Scientific (1 mentions)
Rabbit anti apkc, by Santa Cruz Biotechnology (1 mentions)
Rat anti mira, by Abcam (1 mentions)
2 protocols using mouse anti mira
1
Immunostaining of Larval Drosophila Tissues
2
Immunostaining Larval and Pupal Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Larval and pupal brains were dissected out in phosphate buffered saline (PBS), fixed for 20 min in 4% formaldehyde in PBS and rinsed in 0.2% PBST (PBS + 0.2% TritonX‐100). For immunostaining, brains were incubated with primary antibodies overnight at 4°C, followed by an overnight secondary antibody incubation at 4°C. Samples were mounted in 80% glycerol in PBS for image acquisition. The primary antibodies used were mouse anti‐Mira (1:50; gift of Alex Gould), rat anti‐Mira (1:100, Abcam), rat anti‐pH3 (1:500; Abcam), chick anti‐GFP (1:2000; Abcam), rabbit anti‐RFP (1:100, Abcam), mouse anti‐Repo (1:50, DSHB), guineapig anti‐Hth (1:100, Claude Desplan), mouse anti‐Ey (1:50, DSHB), guineapig anti‐Slp (1:500, Kuniaki Saito), rabbit anti‐D (1:1,000, Steve Russell), guineapig anti‐Tll (1:100, Kuniaki Saito), rabbit anti‐Tll (1:100, Kuniaki Saito) and guineapig anti‐Toy (1:50, Uwe Walldorf). Secondary donkey antibodies conjugated to Alexa 555 and Alexa 647, and goat antibody conjugated to Alexa 405, 488, 555 and 647 (Molecular Probes) were used at 1:500.
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