The largest database of trusted experimental protocols

3 protocols using chloroauric acid trihydrate

1

Biomaterial Fabrication and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyethyleneimine
solution (PEI, ∼50%
in H2O), poly-l-lysine hydrobromide (PLL, MW:
30 000–70 000), Hep ammonium salt from porcine
intestinal mucosa (17–19 kDa), and bovine serum albumin were
all purchased from Sigma-Aldrich (USA). FGF Basic Recombinant Human
Protein and BMP-2 Recombinant Human Protein were obtained from Gibco.
Chloroauric acid trihydrate (HAuCl4·4H2O, 99.9%) was purchased from Shanghai Sinopharm (China). Trisodium
citrate dihydrate (Na3C6H5O7·2H2O) was obtained from Tianjin Kaitong chemical
reagent co., Ltd (China). APTT kits were purchased from MACCURA, China.
PLA sheets with a thickness of 100 μm were purchased from the
Institute of Medical Devices of Shandong Province (China). All of
the chemical reagents were used as received without further purification.
The water used in this work was purified using a Millipore Milli-Q
system.
+ Open protocol
+ Expand
2

Synthesis and Characterization of Multifunctional Nanomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chloroauric acid trihydrate (HAuCl4·3H2O), tetraethyl orthosilicate (TEOS), and cetyltrimethylammonium bromide (CTAB) were obtained from Sinopharm Group Co. Ltd. (Shanghai, People’s Republic of China). ICG was purchased from J&K Chemical Ltd. (Shanghai, People’s Republic of China). MAL-PEG3500-NHS was provided by JenKem Technology Co., Ltd. (Beijing, People’s Republic of China). tLyp-1 peptide (sequence: CGNKRTR) was custom synthesized by GL Biochem (Shanghai, People’s Republic of China). Calcein-AM was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rhodamine B isothiocyanate (RBITC) was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Annexin V-FITC/PI kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, People’s Republic of China). All other chemicals were of analytical or chromatographic grade and were used without further purification.
+ Open protocol
+ Expand
3

Synthesis and Characterization of Gold Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals including chloroauric acid trihydrate (HAuCl4·3H2O), sodium citrate, sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), 10 % sodium azide (NaN3), Tris(hydroxymethyl)aminomethane (Tris-HCl), isopropyl-β-d-thiogalactoside (IPTG), Triton, ammonium sulfate ((NH4)2SO4), sodium dodecyl sulfate (SDS), glycerol, glycine, and Tween-20, were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Bovine serum albumin (BSA), Freund’s adjuvant complete and Freund’s adjuvant incomplete were purchased from Sigma-Aldrich (Sigma-Aldrich St Louis, MO, USA). Horseradish peroxidase (HRP) conjugated anti-mouse antibody, 3, 3′ 5, 5′-tetramethylbenzidine (TMB), PE/Cy5 anti-mouse CD8a, and FITC-CD4 anti-mouse CD4 were purchased from Ebioscience Biotechnology (USA). High affinity purification purified CpG ODNs (5′-TCCATGACGTTCCTGACGTT-SH-3′) were synthesized by Sangon Biotech Co., Ltd (Shanghai, China). Unless otherwise noted, all chemicals were analytical grade and used without further purification. Milli-Q water (less than 18.2 MΩ cm) was used throughout the whole experiment.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!