Goat anti pola1 g 16

Manufactured by Santa Cruz Biotechnology

Goat anti-POLA1 (G-16) is a primary antibody produced in goats and directed against the POLA1 protein. POLA1 is the catalytic subunit of DNA polymerase alpha, an enzyme involved in DNA replication. This antibody can be used to detect and study the POLA1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Ab17725, by Fortis Life Sciences (2 mentions) Mouse anti lamin a c, by Santa Cruz Biotechnology (2 mentions) Mouse anti β catenin cl14, by BD (2 mentions) Mouse anti pcna pc10, by Santa Cruz Biotechnology (2 mentions) Goat anti pold1 c 20, by Santa Cruz Biotechnology (2 mentions) Ab1791, by Abcam (1 mentions) Ab1791, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin ac 15, by Merck Group (1 mentions)

2 protocols using goat anti pola1 g 16

Comprehensive Immunoblotting of DNA Repair Proteins

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Samples were subjected to SDS-PAGE followed by PVDF membrane transfer. Membranes were immunoblotted with the following antibodies: mouse anti-β-actin (AC-15, Sigma #A5441), mouse anti-β-catenin (cl14, BD Biosciences, #610153), rabbit anti-histone H2B (V119, Cell Signaling #8135), rabbit anti-histone H3 (Abcam #ab1791), mouse anti-histone H4 (Abcam #ab31830), mouse anti-LaminA/C (Santa Cruz #sc-7292), mouse anti-PCNA (PC10, Santa Cruz #sc-56), goat anti-POLA1 (G-16, Santa Cruz #sc-5921), goat anti-POLD1 (C-20, Santa Cruz #sc-8797), rabbit anti-polκ (Bethyl #A301-975A), rabbit anti-polη (Abcam #ab17725, Bethyl #A301-231A), mouse anti-polη (B-7, Santa Cruz #sc-17770), rabbit anti-PrimPol (kind gift from L. Blanco), rabbit anti-RAD51 (Calbiochem #PC130-100UL), mouse anti-RPA32 (Calbiochem #NA19L) and mouse anti-RPA70 (Calbiochem #NA13).

Benureau Y., Pouvelle C., Dupaigne P., Baconnais S., Moreira Tavares E., Mazón G., Despras E., Le Cam E, & Kannouche P.L. (2022). Changes in the architecture and abundance of replication intermediates delineate the chronology of DNA damage tolerance pathways at UV-stalled replication forks in human cells. Nucleic Acids Research, 50(17), 9909-9929.

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Immunoblotting Diverse Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were subjected to SDS-PAGE followed by PVDF membrane transfer. Membranes were immunoblotted with the following antibodies: mouse anti-β-actin (AC-15, Sigma #A5441), mouse anti-β-catenin (cl14, BD Biosciences, #610153), rabbit anti-histone H2B (V119, Cell Signaling #8135), rabbit anti-histone H3 (Abcam #ab1791), mouse anti-LaminA/C (Santa Cruz #sc-7292), mouse anti-PCNA (PC10, Santa Cruz #sc-56), goat anti-POLA1 (G-16, Santa Cruz #sc-5921), goat anti-POLD1 (C-20, Santa Cruz #sc-8797), rabbit anti-polκ (Bethyl #A301-975A), rabbit anti-polh (Abcam #ab17725, Bethyl #A301-231A), mouse anti-polh (B-7, Santa Cruz #sc-17770), rabbit-anti PrimPol (kind gift from L. Blanco), rabbit anti-RAD51 (Calbiochem #PC130-100UL), mouse anti-RPA32 (Calbiochem #NA19L) and mouse anti-RPA70 (Calbiochem #NA13).

Benureau Y., Pouvelle C., Tavares E.M., Dupaigne P., Despras E., Cam É.L., & Kannouche P. (2020). Replication intermediate architecture reveals the chronology of DNA damage tolerance pathways at UV-stalled replication forks in human cells.

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