Human PCa LNCaP (American Type Culture Collection (ATCC), 2005), VCaP (ATCC, 2010), and LNCaP C4-2B (C4-2B) [derived from LNCaP tumors grown in a castrated host 23 (link)] cell lines were validated by short tandem repeat DNA fingerprinting with the AmpFℓSTR Identifier kit (Applied Biosystems) in MD Anderson’s Cell Line Core Facility. CCX771 (CXCR7-specific inhibitor), and CCX704 (control for CCX771, i.e., a close analog of CXCR7 without affinity for CXCR7) were provided by ChemoCentryx, Inc. (Mountain View, CA). AMD3100 (CXCR4 inhibitor) and ENZ (MDV3100) were purchased from SelleckChem, and SDF1 was purchased from GeneScript.
Enz mdv3100
Manufactured by Selleck Chemicals
Sourced in United States
ENZ (MDV3100) is a selective androgen receptor modulator (SARM) that is used in research applications. It functions by binding to and modulating the activity of the androgen receptor. The core function of ENZ (MDV3100) is to serve as a research tool for studying the role of the androgen receptor in various biological processes.
Automatically generated - may contain errors
3 protocols using enz mdv3100
1
Validating PCa Cell Lines and CXCR7/CXCR4 Inhibitors
2
Prostate Cancer Cell Line Characterization
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Human PCa LNCaP (American Type Culture Collection [ATCC], 2005), VCaP (ATCC, 2010) and LNCaP C4‐2B (C4‐2B) (derived from LNCaP tumors grown in a castrated host23 ) cell lines were validated by short tandem repeat DNA fingerprinting with the AmpFℓSTR Identifier kit (Applied Biosystems) in MD Anderson's Cell Line Core Facility. CCX771 (CXCR7‐specific inhibitor), and CCX704 (control for CCX771, i.e., a close analog of CXCR7 without affinity for CXCR7) were provided by ChemoCentryx (Mountain View, CA). AMD3100 (CXCR4 inhibitor) and ENZ (MDV3100) were purchased from SelleckChem, and SDF1 was purchased from GeneScript.
3
Combination ENZ and ATO effects on PCa
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The human PCa cell line C4-2B was obtained from the American Type Culture Collection (Manassas, VA, USA). All cells were cultured in RPMI-1640 medium supplemented with 10% complete fetal bovine serum (FBS) and maintained at 37 °C in a humidified incubator with 5% CO 2 .
In vitro ENZ and ATO treatment ENZ (MDV3100) was purchased from SellekChem (Houston, TX, USA) and diluted in dimethyl sulfoxide (DMSO) at 1 μM for in vitro experiments. ATO was purchased from Sigma (St. Louis, MO, USA) and diluted in DMSO at 5 μM for in vitro experiments.
C4-2B cells were pretreated with 5% DMSO vehicle control for 24 h. On the following days, added additional drug combinations into cells and then incubated for 48 h as follows: 1 μM ENZ, 5 μM ATO, or their combination. The cells were collected and prepared for fluorescence-activated cell sorting (FACS), DNA fragmentation detection, and western blot (WB) analysis.
In vitro ENZ and ATO treatment ENZ (MDV3100) was purchased from SellekChem (Houston, TX, USA) and diluted in dimethyl sulfoxide (DMSO) at 1 μM for in vitro experiments. ATO was purchased from Sigma (St. Louis, MO, USA) and diluted in DMSO at 5 μM for in vitro experiments.
C4-2B cells were pretreated with 5% DMSO vehicle control for 24 h. On the following days, added additional drug combinations into cells and then incubated for 48 h as follows: 1 μM ENZ, 5 μM ATO, or their combination. The cells were collected and prepared for fluorescence-activated cell sorting (FACS), DNA fragmentation detection, and western blot (WB) analysis.
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