Ae1 ae3

Manufactured by Agilent Technologies

Sourced in Denmark, United States

The AE1/AE3 is a high-performance UV-Vis spectrophotometer designed for laboratory use. It provides accurate and reliable measurements of absorption and transmission spectra in the ultraviolet and visible light range.

Automatically generated - may contain errors

Lab products found in correlation

Ov tl 12 30, by Agilent Technologies (3 mentions) Mrq 50, by Cell Marque (2 mentions) Mrq37, by Cell Marque (2 mentions) Ventana benchmark xt, by Roche (2 mentions) Hmb45, by Agilent Technologies (2 mentions) Mib 1, by Agilent Technologies (2 mentions) Ema e29, by Agilent Technologies (2 mentions) Spectral dapi, by Akoya Biosciences (2 mentions) Bond rxm research stainer, by Leica Biosystems (2 mentions) Diamond antifade mounting medium, by Thermo Fisher Scientific (2 mentions) Vector red alkaline phosphatase substrate kit, by Vector Laboratories (1 mentions) Histo zyme, by Diagnostic BioSystems (1 mentions) Clone 16, by Leica Biosystems (1 mentions) Leica bond max system, by Leica Biosystems (1 mentions) Cd20 l26, by Leica Biosystems (1 mentions) I view dab universal kit, by Roche (1 mentions) Cd34 qbend 10, by Roche (1 mentions) Qbend 10, by Roche (1 mentions) Ventana benchmark xt automated system, by Roche (1 mentions) Synaptophysin, by Agilent Technologies (1 mentions)

48 protocols using ae1 ae3

Microdissection of Breast Cancer Tissue Samples

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Individual 10-μm-thick formalin-fixed paraffin-embedded (FFPE) specimens of surgically resected BC tissue were placed on Leica foil membrane slides, and immunohistochemically stained by pan-cytokeratin antibody cocktails (AE1/AE3, Dako, Glostrup, Denmark, M3515). Histo/Zyme (Diagnostic BioSystems, Pleasanton, CA, USA; DBS-K046-15) was used for antigen retrieval, and VECTOR Red Alkaline Phosphatase Substrate Kit (VECTOR Laboratories, Burlingame, CA, USA; SK-5100) was used for visualization. LMD of the stained FFPE slides was performed using LMD7000 systems (Leica microsystems, Wetzlar, Gemany). Cancer cell clusters from the BC samples were selectively microdissected (Additional file 3: Figure S1). Normal samples obtained from adjacent normal mammary epithelia and intraductal papilloma epithelia were also microdissected. Adjacent normal epithelia from 10 patients were pooled as a single sample.

Uehiro N., Sato F., Pu F., Tanaka S., Kawashima M., Kawaguchi K., Sugimoto M., Saji S, & Toi M. (2016). Circulating cell-free DNA-based epigenetic assay can detect early breast cancer. Breast Cancer Research : BCR, 18, 129.

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Comprehensive Tumor Tissue Analysis

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Sectioned tumors were mounted and stained with hematoxylin and eosin, human cytokeratin antibody clones AE1/AE3 (Dako North America), MMP9 antibody #3852 (Cell Signaling), and CD31 antibody (PECAM-1 M-20; Santa Cruz #sc-1506). Slides were scanned and analyzed using Aperio ImageScope Software algorithms for staining intensity (MMP9) and number of positive cell groups (CD31).

Mehner C., Hockla A., Miller E., Ran S., Radisky D.C, & Radisky E.S. (2014). Tumor cell-produced matrix metalloproteinase 9 (MMP-9) drives malignant progression and metastasis of basal-like triple negative breast cancer. Oncotarget, 5(9), 2736-2749.

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Immunohistochemistry Profiling of Tissue Samples

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Immunohistochemistry was performed on 4-μm sections from formalin-fixed, paraffin-embedded (FFPE) tissue using a Ventana Benchmark XT automated stainer (Ventana Medical Systems, Tucson, AZ). The following antibodies were used: Melan-A (monoclonal mouse anti-human anti-body, A103; DAKO; 1:100), TFE3 (monoclonal mouse anti-human antibody, MRQ37, Cell Marque; prediluted), PAX8 (monoclonal mouse anti-human antibody, MRQ50, Cell Marque; prediluted), cytokeratin AE1/AE3 (monoclonal mouse anti-human antibody, AE1/AE3, Ventana; prediluted), cytokeratin 7 (monoclonal mouse anti-human antibody, OV-TL 12/30, Dako; 1:800), CAM 5.2 (monoclonal mouse anti-human antibody, BD, 1:100), Cathepsin K (monoclonal mouse anti-human antibody, 3F9, Cell marque, prediluted) and HBM45 (monoclonal mouse antihuman antibody, Dako; 1:60). The corresponding positive and negative controls were shown to be adequate.

Pei J., Cooper H., Flieder D.B., Talarchek J.N., Al-Saleem T., Uzzo R.G., Dulaimi E., Patchefsky A.S., Testa J.R, & Wei S. (2019). NEAT1-TFE3 and KAT6A-TFE3 renal cell carcinomas, new members of MiT family translocation renal cell carcinoma. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(5), 710-716.

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Immunohistochemical analysis of tumors

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Sections (4 μm thick) from formalin-fixed and paraffin-embedded blocks were prepared for IHC and Epstein-Barr encoded RNA in situ hybridization (EBER ISH). IHC was performed using a Ventana XT automated stainer (Ventana Corporation, Tucson, AZ, USA) with antibodies against CK (1:300, AE1/AE3; DAKO, Carpinteria, CA, USA), CD3 (1:100, DAKO), and CD20 (1:1600, DAKO). Every other week, there was a regular meeting with the surgeon, the pathologist, and researchers for reviewing and discussion of the histopathological characteristics of each tumour.

Choi Y.Y., Lee J.E., Kim H., Sim M.H., Kim K.K., Lee G., Kim H.I., An J.Y., Hyung W.J., Kim C.B., Noh S.H., Kim S, & Cheong J.H. (2016). Establishment and characterisation of patient-derived xenografts as paraclinical models for gastric cancer. Scientific Reports, 6, 22172.

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Histopathologic Evaluation of Neoadjuvant Therapy

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The histopathologic response to neoadjuvant therapy was evaluated based on pathologic examination of resected specimens, including uterine tissue, the vaginal cuff, the parametrium, bilateral adnexa, and pelvic lymph nodes. The status of resected lymph nodes was described in terms of lymph-node involvement (involved or not involved).
The pan-cytokeratin antibody (AE1/AE3) (Dako, Glostrup, Denmark) was used in immunohistochemical staining to identify any invisible residual tumor cells.

Lv Y., Wang N., Liu Y., Li X., Fan L., Li M., Wang L., Yu Z., Yan Q., Guo Y., Guo S., Wei L., Shi M, & Wang Z. (2015). Tumor invasion depth is a useful pathologic assessment for predicting outcomes in cervical squamous cell carcinoma after neoadjuvant radiotherapy. Diagnostic Pathology, 10, 200.

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Immunohistochemical Profiling of Tumors

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Immunohistochemical (IHC) staining was performed on representative 4-μm-thick formalin-fixed, paraffin-embedded (FFPE) tumor tissue blocks for the five cases using the Leica BOND-MAX™ system (Leica Biosystems, Wetzlar, Germany) according to the manufacturer’s protocol. We used antibodies for the ER (6F11, 1:300, Novocastra, New Castle, United Kingdom, 1:300), PR (clone 16, Novocastra, 1:1200), premelanosome protein (HMB-45, Dako, Carpinteria, CA, USA, 1:80), cytokeratin (PAN CK) (AE1/AE3, Dako, 1:500), CD 20 (L26, Novocastra, 1:800), CD 3 (polyclonal, Dako, 1:300), leukocyte common antigen (CD 45 RB) (2B11 + PD7/26, Dako, 1:1000) and CD 56 (CD564, Novocastra, 1:200).

Lee H.W., Lee H., Park C., Oh W.J., Kim T.J., Kwon G.Y, & Seo S.I. (2021). Pattern of Tumor-Infiltrating Lymphocytes in Mixed Epithelial and Stromal Tumor of the Kidney: A Review of Five Cases. Cells, 10(4), 917.

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Histological Characterization of Engrafted Tumors

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The engrafted tumors were fixed with 10% phosphate-buffered formalin, and paraffin-embedded sections were stained using hematoxylin and eosin. Anti-human vimentin (Clone V9; #M0725; Dako, Glostrup, Denmark) and anti-human pancytokeratin (AE1/AE3; #M3515; Dako) antibodies were used for the confirmation of human cell-derived tumors. A staining without primary antibodies was also prepared as a control for nonspecific effects of primary antibodies. Secondary antibodies (I-VIEW DAB Universal Kit, #518100032) were purchased from Roche Diagnostics KK (Tokyo, Japan).

Kanaji N., Tadokoro A., Susaki K., Yokokura S., Ohmichi K., Haba R., Watanabe N., Bandoh S., Ishii T., Dobashi H, & Matsunaga T. (2014). Higher susceptibility of NOD/LtSz-scid Il2rg−/− NSG mice to xenotransplanted lung cancer cell lines. Cancer Management and Research, 6, 431-436.

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Immunohistochemical Staining Protocol

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Staining was performed using standardized techniques for desmin (D33; Dako), smooth muscle actin (1A4; Dako), S100 (polyclonal; Dako), CD34 (QBEnd/10; Roche), pancytokeratin (AE1/AE3; Dako), CK7 (OV-TL 12/30; Dako), CK20 (Ks20.8; Dako), epithelial membrane antigen (E29; Roche), and INI-1 (MRQ-27; Roche). On-slide positive controls were used throughout.

Lacambra M.D., Antonescu C.R., Chit C., Chiu W.K., Demicco E.G., Ferguson P.C., Swanson D., To K.F., Zhang L, & Dickson B.C. (2022). EXPANDING THE SPECTRUM OF MESENCHYMAL NEOPLASMS WITH NR1D1-REARRANGEMENT. Genes, chromosomes & cancer, 61(7), 420-426.

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Immunohistochemical Characterization of Tissues

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Staining was performed on a Dako OMNIS (Aligent, Santa Clara, CA) using standard techniques for CD34 (QBEnd/10; Roche), desmin (D33; Dako), epithelial membrane antigen (E29; Roche), keratin (AE1/AE3; Dako), MyoD1 (5.8A; Dako), myogenin (MyG007; Biocare), smooth muscle actin (1A4; Dako), S100 (polyclonal; Dako). On-slide positive controls were applied throughout.

Han R., Dermawan J.K., Demicco E.G., Ferguson P.C., Griffin A.M., Swanson D., Antonescu C.R, & Dickson B.C. (2022). ZFP64::NCOA3 GENE FUSION DEFINES A NOVEL SUBSET OF SPINDLE CELL RHABDOMYOSARCOMA. Genes, chromosomes & cancer, 61(11), 645-652.

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Immunohistochemical Assessment of Tumor Markers

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Formalin-fixed tumors were embedded in paraffin using standard procedures. The 5-µm sections were processed and stained with antibodies directed against PRKD2 (1:250; Abcam #ab51250); pan-cytokeratin (1:80; Dako, clone AE1/AE3); desmin (1:80; Dako, clone D33); von Willebrand Factor VIII (1:100; Biocare Medical, #CP039B); and Ki-67 (1:100; Dako, clone MIB-1). Apoptotic cells were detected by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) using the In Situ Cell Death Detection Kit, POD (Roche, # 11684817910) and quantified by counting >700 cells from at least four microscopic fields.

Azoitei N., Diepold K., Brunner C., Rouhi A., Genze F., Becher A., Kestler H., van Lint J., Chiosis G., Koren J I.I.I., Fröhling S., Scholl C, & Seufferlein T. (2014). HSP90 Supports Tumor Growth and Angiogenesis through PRKD2 Protein Stabilization. Cancer research, 74(23), 7125-7136.

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