Acquity beh130 c18 column

Manufactured by Waters Corporation

Sourced in United Kingdom

The Acquity BEH130 C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features a 1.7 μm particle size and a 130 Å pore size, providing efficient and high-resolution separations. The C18 stationary phase offers excellent retention and selectivity for a variety of analytes.

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Lab products found in correlation

Xevo tq mass spectrometer, by Waters Corporation (1 mentions) Nanoacquity uplc, by Waters Corporation (1 mentions) Nanoacquity uplc system, by Waters Corporation (1 mentions)

Spelling variants of this product

C18 column (384 citations) BEH130 C18 (41 citations) BEH130 C18 column (34 citations) BEH130 (8 citations) Acquity UPLC BEH130 C18 column (6 citations) C18 Acquity column (2 citations) Acquity M-Class BEH130 C18 analytical column (2 citations)

2 protocols using acquity beh130 c18 column

Quantitative Metabolite Profiling via UPLC-MS

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Initial method development and
was performed using a nanoAcquity UPLC coupled via electrospray ionization
to a Xevo TQ mass spectrometer (Waters Corporation). Briefly, 1 μL
of each sample was injected directly onto a 150 μm × 100
mm 1.7 μm Acquity BEH130 C18 column (Waters) using a 23 min
gradient of 3–40% MeCN at a flow rate of 1.8 μL/min with
a column temperature of 35 °C. Data were analyzed using Skyline.^{36 (link)}

Foster M.W., Thompson J.W., Ledford J.G., Dubois L.G., Hollingsworth J.W., Francisco D., Tanyaratsrisakul S., Voelker D.R., Kraft M., Moseley M.A, & Foster W.M. (2014). Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A. Journal of Proteome Research, 13(8), 3722-3732.

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Optimized Gradient Separation for LC-MS

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A binary gradient was developed on a Waters nanoAcquity UPLC system (Milford, MA, USA). Trapping column: 180 µm x 20 mm; 5 µm Symmetry C 18 ; analytical column: 100 µm x 100 mm; 1.7 µm Acquity BEH130 C 18 column (both Waters; Manchester, UK). Column temperature was kept at 30 ˚C. The gradient method used acidified H 2 O (solvent A; 0.1 % FA) and ACN (solvent B) for analysis as well as for wash purposes. The gradient had a run time of 35 minutes, starting with 1.00 minute of on-column focusing. The gradient went from 1 to 50% B over 5.00 minutes, followed by 0.99 minutes of washing (99 % B) and 24 minutes of equilibration at initial conditions. Injection: 5 µL full loop (overfill factor 1).

Boschmans J., Lemière F, & Sobott F. (2017). Analyzing complex mixtures of drug-like molecules: Ion mobility as an adjunct to existing liquid chromatography-(tandem) mass spectrometry methods. Journal of chromatography. A, 1490.

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