Hematoxylin-eosin and Sirius red staining were performed according to standard protocols. For immunohistochemical analysis, liver specimens were fixed in 10% buffered formalin or 4% paraformaldehyde and incubated with rat anti-mouse F4/80 (Clone: A3-1, AbD Serotec, Raleigh, NC, USA), rat anti-mouse CD68 (Clone: FA-11, AbD Serotec), goat anti-mouse Gpnmb (Clone: #297310, R&D Systems, Minneapolis, MN, USA), and monoclonal antibody against α-SMA (Clone: E184, Merck Millipore, Billerica, MA, USA). For immunofluorescent staining, paraffin sections were incubated with rat anti-mouse CD68 (Clone: FA-11, AbD Serotec) and goat anti-mouse Gpnmb (Clone: #297310, R&D Systems). These samples then imaged with fluorescent microscopy. The terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling assay (Promega, Madison, WI, USA) was performed on paraffin liver sections according to the manufacturer’s instructions. All samples undergoing immunofluorescent staining were labeled with ProLongR Gold Antifade Mountant with DAPI (Thermo Fisher Scientific Inc., Grand Island, NY, USA). Serum levels of alanine aminotransferase (ALT) were measured with a commercial kit (SRL, Tokyo, Japan).
Monoclonal antibody against α sma
Manufactured by Merck Group
Sourced in United States
Monoclonal antibody against α-SMA is a laboratory reagent used for the detection and quantification of alpha-smooth muscle actin (α-SMA) in biological samples. α-SMA is a widely used marker for the identification of myofibroblasts and is involved in various physiological and pathological processes. This monoclonal antibody provides a specific and sensitive tool for researchers to study α-SMA expression and distribution in their samples.
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Lab products found in correlation
2 protocols using monoclonal antibody against α sma
1
Quantitative Histological and Biochemical Assessment of Liver Injury
2
Investigating Thick TBM Duplication via Microscopy
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To investigate what the essence of thick TBM duplication was, we performed low-vacuum scanning electron microscopic (Hitachi, Tokyo, Japan) analysis and immunofluorescence (IF) analysis in the representative cases with or without pathogenic mutations. For low-vacuum scanning electron microscopic analysis, 5-μm formalin-fixed paraffin-embedded sections were stained with periodic acid–methenamine silver to evaluate the TBM as described previously.19 (link),20 (link) For IF analysis, monoclonal antibody against α-SMA (Merck KGaA, Darmstadt, Germany) for myofibroblast and Phaseolus vulgaris (Vector Laboratories, Inc., Burlingame, CA) for proximal tubules, respectively, were used in formalin-fixed paraffin-embedded sections. Streptavidin, Alexa Fluor 488 conjugate and goat antimouse IgG (H+L; Thermo Fisher Scientific Inc, Waltham, MA), and Alexa Fluor 568 (Thermo Fisher Scientific Inc) were used, and sections were counterstained with 4′,6-diamidino-2-phenylindole. IF images were captured using BZ-X800 (Keyence, Osaka, Japan).
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